Advertisement

Archives of Virology

, Volume 149, Issue 3, pp 447–464 | Cite as

Virus spread, tissue inflammation and antiviral response in brains of flavivirus susceptible and resistant mice acutely infected with Murray Valley encephalitis virus

  • O. J. Silvia
  • L. Pantelic
  • J. S. Mackenzie
  • G. R. Shellam
  • J. Papadimitriou
  • N. Urosevic
Article

Summary.

Inborn resistance to flaviviruses, conferred by a single chromosome 5 locus Flv, is a genetic trait operative in wild mice and a few strains of laboratory mice. In this study we have used in situ hybridisation to trace the spread of flavivirus genomic RNA within the brains of flavivirus susceptible C3H/HeJARC and congenic resistant C3H.PRI-Flv r mice following infection with Murray Valley encephalitis virus (MVE) in parallel to studying a brain histopathology and induction of cellular genes involved in antiviral response. We find that in contrast to a high viral RNA content in brains of susceptible mice, viral RNA was markedly reduced in the cortex, olfactory bulb, thalamus and hypothalamus of resistant mice. Trace amounts of viral RNA were detected in the medulla oblongata while it was completely absent from the hippocampus, pons and cerebellum of resistant mice at different time points post infection. The low virus titres within brains of resistant mice coincided with a very mild inflammation, low counts of infiltrating inflammatory cells, and lower IFN I/II and TNFα gene induction than in susceptible mice. Furthermore, transcripts of several genes belonging to a 2′,5′-oligoadenylate synthetase (OAS) family, implicated in IFN I-inducible OAS/RNase L antiviral pathway, showed similar brain tissue induction in both strains of mice suggesting only minor contribution of this pathway to the resistance phenotype.

Keywords

Olfactory Bulb Medulla Oblongata Antiviral Response Wild Mouse Trait Operative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag/Wien 2003

Authors and Affiliations

  • O. J. Silvia
    • 1
  • L. Pantelic
    • 1
  • J. S. Mackenzie
    • 3
  • G. R. Shellam
    • 1
  • J. Papadimitriou
    • 2
  • N. Urosevic
    • 1
  1. 1.Microbiology, School of Biomedical and Chemical SciencesThe University of Western AustraliaNedlandsAustralia
  2. 2.School of Surgery and PathologyThe University of Western AustraliaNedlandsAustralia
  3. 3.Department of Microbiology and ParasitologyThe University of QueenslandSt. LuciaAustralia

Personalised recommendations