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Archives of Virology

, Volume 148, Issue 4, pp 677–691 | Cite as

Early detection of PrPres in BSE-infected bovine PrP transgenic mice

  • J. Castilla
  • A. Gutiérrez Adán
  • A. Brun
  • B. Pintado
  • M. A. Ramírez
  • B. Parra
  • D. Doyle
  • M. Rogers
  • F. J. Salguero
  • C. Sánchez
  • J. M. Sánchez-Vizcaíno
  • J. M. Torres

Summary.

 Transgenic mouse lines expressing different levels of the bovine prion protein gene (boPrPC) were generated. Upon infection with BSE prions, all transgenic lines tested exhibited characteristics of the bovine disease. Typical CNS spongiform degeneration was observed by histopathology and presence of PrPres could be detected both by Western blot and immunohistochemistry (IHC) assays, confirming for this model the absence of an interspecies barrier to BSE infection. Differences in incubation times post-inoculation depend upon the expression level of boPrPC and the amount of prions in the inoculum. In the absence of clinical signs, pathognomonic markers of disease could be detected as early as 150 or 196 days post-inoculation by IHC and Western blot analysis, respectively. This result indicates that prion infectivity in experimental mouse bioassays can be measured earlier by assessing immunologically the presence of PrPres in brains from inoculated animals. Although these transgenic mice were also susceptible to sheep scrapie prion infection, the extent of incubation times was considerably longer and PrPres was detected in only 70 % of inoculated mice. Interestingly, transgenic mice-propagated sheep scrapie prions displayed distinct biochemical properties when compared to both the original sheep scrapie and transgenic mouse-propagated BSE inoculum.

Keywords

Transgenic Mouse Transgenic Line Incubation Time Prion Protein Mouse Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2002

Authors and Affiliations

  • J. Castilla
    • 1
  • A. Gutiérrez Adán
    • 2
  • A. Brun
    • 1
  • B. Pintado
    • 2
  • M. A. Ramírez
    • 1
  • B. Parra
    • 1
  • D. Doyle
    • 3
  • M. Rogers
    • 3
  • F. J. Salguero
    • 1
  • C. Sánchez
    • 1
  • J. M. Sánchez-Vizcaíno
    • 1
  • J. M. Torres
    • 1
  1. 1.Centro de Investigación en Sanidad Animal, INIA, Madrid, SpainES
  2. 2.Departamento de Reproducción Animal y Conservación de Recursos Zoogenéticos, Madrid, SpainES
  3. 3.University College Dublin, Department of Zoology and Conway Institute for Biomolecular and Biomedical Research, Dublin, Republic of IrelandIE

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