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Archives of Virology

, Volume 148, Issue 4, pp 693–706 | Cite as

Genotyping field strains of African swine fever virus by partial p72 gene characterisation

  • A. D. S. Bastos
  • M.-L. Penrith
  • C. Crucière
  • J. L. Edrich
  • G. Hutchings
  • F. Roger
  • E. Couacy-Hymann
  • G. R.Thomson

Summary.

 A PCR-based sequencing method was developed which permits detection and characterization of African swine fever virus (ASFV) variants within 5 and 48 h, respectively, of receipt of a clinical specimen. Amplification of a 478 bp fragment corresponding to the C-terminal end of the p72 gene, confirms virus presence with genetic characterization being achieved by nucleotide sequence determination and phylogenetic analysis. The method was applied to 55 viruses including those representative of the major ASF lineages identified previously by restriction fragment length polymorphism (RFLP) analysis. Results confirmed that the p72 genotyping method identifies the same major viral groupings. Characterization of additional viruses of diverse geographical, species and temporal origin using the PCR-based method indicated the presence of ten major ASF genotypes on the African continent, the largest of which comprised a group of genetically homogeneous viruses recovered from outbreaks in Europe, South America, the Caribbean and West Africa (the ESAC-WA genotype). In contrast, viruses from southern and East African countries were heterogeneous, with multiple genotypes being present within individual countries. This study provides a rapid and accurate means of determining the genotype of field and outbreak strains of ASF and is therefore useful for molecular epidemiological clarification of ASF.

Keywords

Restriction Fragment Length Polymorphism African Continent African Swine Fever Virus Viral Grouping Outbreak Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2002

Authors and Affiliations

  • A. D. S. Bastos
    • 1
  • M.-L. Penrith
    • 1
  • C. Crucière
    • 3
  • J. L. Edrich
    • 2
  • G. Hutchings
    • 4
  • F. Roger
    • 5
  • E. Couacy-Hymann
    • 6
  • G. R.Thomson
    • 1
  1. 1.ARC-Onderstepoort Veterinary Institute, Onderstepoort, South AfricaZA
  2. 2.Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, South AfricaZA
  3. 3.AFSSA-Lerpaz, Maisons-Alfort, FranceFR
  4. 4.Institute for Animal Health, Pirbright Laboratory, Pirbright, U.K.GB
  5. 5.CIRAD-EMVT, Délégation CIRAD, Antananarivo, MadagascarMG
  6. 6.Lanada/Lcpa, Bingerville, Côte d’Ivoire

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