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Archives of Virology

, Volume 148, Issue 2, pp 389–397 | Cite as

Complete nucleotide sequence and genome organization of a Chinese isolate of tobacco bushy top virus*

  • X. H. Mo
  • X. Y. Qin
  • J. Wu
  • C. Yang
  • J. Y. Wu
  • Y. Q. Duan
  • T. F. Li
  • H. R. Chen
Brief Report

Summary.

 The complete nucleotide sequence of a Chinese isolate of tobacco bushy top virus (TBTV), designated TBTV-Ch, was determined from cDNA generated from double-stranded RNA extracted from diseased tobacco. The genome is 4152 nucleotides (nt) in size, contains four putative open reading frames (ORFs) and untranslated regions of 10 nt and 645 nt at the 5′ and 3′ ends, respectively. In genome organization and in the amino acid sequence of its potential products, the RNA of TBTV-Ch is similar to other umbraviruses sequenced to date. The results suggested that TBTV should be regarded as a definitive species of the genus Umbravirus.

Keywords

Nucleotide Amino Acid Sequence Nucleotide Sequence Untranslated Region Genome Organization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2002

Authors and Affiliations

  • X. H. Mo
    • 1
  • X. Y. Qin
    • 1
  • J. Wu
    • 1
  • C. Yang
    • 3
  • J. Y. Wu
    • 2
  • Y. Q. Duan
    • 1
  • T. F. Li
    • 1
  • H. R. Chen
    • 2
  1. 1.Biotechnology Laboratory, Yunnan Academy of Tobacco Science, Kunming, P. R. ChinaCN
  2. 2.Yunnan Agricultural University, Kunming, P. R. ChinaCN
  3. 3.Dali Tobacco Research Institute, Dali, P. R. ChinaCN

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