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Archives of Virology

, Volume 147, Issue 11, pp 2207–2214 | Cite as

Nucleotide sequence and taxonomy of Cycas necrotic stunt virus

Brief Report
  • S. S. Han
  • A. V. Karasev
  • H. Ieki
  • T. Iwanami

Summary.

Cycas necrotic stunt virus (CNSV) is the only well-characterized virus from gymnosperm. cDNA segments corresponding to the bipartite genome RNAs (RNA1, RNA2) were synthesized and sequenced. Each RNA encoded a single polyprotein, flanked by the 5′ and 3′ non-coding regions (NCR) and followed by a poly (A) tail. The putative polyproteins encoded by RNA1 and RNA2 had sets of motifs, which were characteristic of viruses in the genus Nepovirus. The polyproteins showed higher sequence identities to Artichoke Italian latent virus, Grapevine chrome mosaic virus and Tomato black ring virus, all of which belong to subgroup b of the genus Nepovirus, than to other nepoviruses. Phylogenetic analysis of RNA dependent RNA polymerase and coat protein also showed closer relationships with these viruses than other viruses. The data obtained supported the taxonomical status of CNSV as a definitive member of the genus Nepovirus, subgroup b.

Keywords

Chrome Mosaic Virus Coat Protein Genome RNAs Taxonomical Status 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2002

Authors and Affiliations

  • S. S. Han
    • 1
  • A. V. Karasev
    • 2
  • H. Ieki
    • 1
  • T. Iwanami
    • 3
  1. 1.National Institute of Fruit Tree Science, Tsukuba, JapanJP
  2. 2.Biotechnology Foundation Laboratories, Thomas Jefferson University, Doylestown, Pennsylvania, U.S.A.US
  3. 3.National Agricultural Research Center for Kyushu and Okinawa Region, Kumamoto, JapanJP

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