Estrogen induces elevation of cAMP-dependent protein kinase activity in immortalized hippocampal neurons: imaging in living cells

Summary.

17β-estradiol-stimulated PKA activation in living differentiated H19-7 cells was visualized using DR2, a cell-permeable fluorescent PKA substrate. Estradiol induced marked changes of fluorescence in cytosolic areas and these were inhibited by a PKA inhibitor.