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Intrapallidal dopamine restores motor deficits induced by 6-hydroxydopamine in the rat

  • A. Galvan
  • B. Floran
  • D. Erlij
  • J. Aceves
Article

Summary

To explore whether dopamine deficits in the globus pallidus have a role in generating the motor symptoms of Parkinson’s disease, we examined the effects of selective intrapallidal administration of dopamine or its antagonists in rats unilaterally lesioned with 6-hydroxydopamine into the medial forebrain bundle. Either the turning behavior induced by apomorphine or the deficit in the performance of a skilled forelimb-reaching task was used as assay for drug action. Microinjection of either the D2 receptor antagonist, sulpiride, or the Dl receptor antagonist, SCH-23390, into the dopamine-denervated pallidum significantly reduced apomorphine induced turning. In animals trained to perform a skilled forelimb-reaching task, 6-OHDA lesions caused a marked motor deficit in the contralateral forelimb. Intrapallidal dopamine applied either intermittently or continuously, restored up to 50% of the motor performance. Continuous application promoted a motor recovery that outlasted dopamine administration. These results show that lack of dopamine in the GP plays an important role in generating the motor symptoms caused by lesion of dopaminergic pathways. Moreover, motor recovery was produced by selectively injecting dopamine into the globus pallidus.

Keywords

Dopamine dopamine Dl receptors dopamine D2 receptors GABA globus pallidus Parkinson’s disease turning behavior staircase test 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • A. Galvan
    • 1
  • B. Floran
    • 1
  • D. Erlij
    • 2
  • J. Aceves
    • 1
    • 3
  1. 1.Departamento de FisiologíaBiofísica y Neurociencias, Centro de Investigatión y de Estudios Avanzados del Instituto Politécnico Nacional, México D. F.México
  2. 2.Department of PhysiologySUNY Downstate Medical CenterBrooklynU.S.A.
  3. 3.Department of PhysiologyCINVESTAV-IPNMéxico D.F.México

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