Advertisement

MicroRNA-425-5p promotes tau phosphorylation and cell apoptosis in Alzheimer’s disease by targeting heat shock protein B8

  • Jiao Yuan
  • Yanpeng Wu
  • Lu Li
  • Chuanqin LiuEmail author
Neurology and Preclinical Neurological Studies - Original Article

Abstract

Alzheimer’s disease (AD) is the most prevalent and age-related dementia accompanied by neurodegenerative disorder, memory loss, and abnormal behaviors. Recent studies have shown an increasing interest in studying the role of microRNAs (miRNAs) and their potential values in the early diagnostics of AD. MiR-425-5p has extensively expression within various tissues and organs, acting as an important regulator in many pathological procedures. The functions of miR-425-5p involved in AD were investigated in the present study. The results showed that miR-425-5p was upregulated in patients with AD and HEK293/tau cells. Transfections with miR-425-5p overexpression vector significantly enhanced cell apoptosis, activated glycogen synthase kinase-3β (GSK-3β), and increased tau phosphorylation in HEK293/tau cells. Heat shock protein B8 (HSPB8) was directly targeted by miR-425-5p. Upregulation of miR-425-5p induced cell apoptosis and promoted tau phosphorylation partially via targeting HSPB8 in AD. Therefore, miR-425-5p might act as a new therapeutic target for AD treatment.

Keywords

MicroRNA-425-5p AD GSK-3β HSPB8 Apoptosis 

Notes

Author contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by JY, YW, LL, and CL. The first draft of the manuscript was written by CL, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical approval was obtained from the Ethics Committee of Qingdao Mental Health Center (No. QMHC2015021156673). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

702_2019_2134_MOESM1_ESM.doc (32 kb)
Supplementary file1 (DOC 31 kb)

References

  1. Absalon S, Kochanek DM, Raghavan V, Krichevsky AM (2013) MiR-26b, upregulated in Alzheimer's disease, activates cell cycle entry, tau-phosphorylation, and apoptosis in postmitotic neurons. J Neurosci 33:14645–14659CrossRefGoogle Scholar
  2. Banzhaf-Strathmann J et al (2014) MicroRNA-125b induces tau hyperphosphorylation and cognitive deficits in Alzheimer's disease. EMBO J. 33:1667–1680CrossRefGoogle Scholar
  3. Crippa V et al (2010a) A role of small heat shock protein B8 (HspB8) in the autophagic removal of misfolded proteins responsible for neurodegenerative diseases. Autophagy 6:958–960.  https://doi.org/10.4161/auto.6.7.13042 CrossRefPubMedGoogle Scholar
  4. Crippa V et al (2010b) The small heat shock protein B8 (HspB8) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS). Hum Mol Genet 19:3440–3456.  https://doi.org/10.1093/hmg/ddq257 CrossRefPubMedGoogle Scholar
  5. Delay C, Mandemakers W, Hébert SS (2012) MicroRNAs in Alzheimer's disease. Neurobiol Dis 46:285–290CrossRefGoogle Scholar
  6. Fang F, Song T, Zhang T, Cui Y, Zhang G, Xiong Q (2017) MiR-425-5p promotes invasion and metastasis of hepatocellular carcinoma cells through SCAI-mediated dysregulation of multiple signaling pathways. Oncotarget 8:31745PubMedPubMedCentralGoogle Scholar
  7. Fontaine JM, Sun X, Hoppe AD, Simon S, Vicart P, Welsh MJ, Benndorf R (2006) Abnormal small heat shock protein interactions involving neuropathy-associated HSP22 (HSPB8) mutants. FASEB J 20:2168–2170.  https://doi.org/10.1096/fj.06-5911fje CrossRefPubMedGoogle Scholar
  8. Geekiyanage H, Chan C (2011) MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid β, novel targets in sporadic Alzheimer's disease. J Neurosci 31:14820–14830CrossRefGoogle Scholar
  9. Geekiyanage H, Jicha GA, Nelson PT, Chan C (2012) Blood serum miRNA: non-invasive biomarkers for Alzheimer's disease. Exp Neurol 235:491–496CrossRefGoogle Scholar
  10. Hamouda MA et al (2014) The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells. Oncotarget 5:6252–6266.  https://doi.org/10.18632/oncotarget.2193 CrossRefPubMedPubMedCentralGoogle Scholar
  11. Hardy JA, Higgins GA (1992) Alzheimer's disease: the amyloid cascade hypothesis. Science 256:184–186CrossRefGoogle Scholar
  12. Irobi J et al (2010) Mutant HSPB8 causes motor neuron-specific neurite degeneration. Hum Mol Genet 19:3254–3265CrossRefGoogle Scholar
  13. Jiang B et al (2017) MicroRNA-126a-5p enhances myocardial ischemia-reperfusion injury through suppressing Hspb8 expression. Oncotarget 8:94172PubMedPubMedCentralGoogle Scholar
  14. Khachaturian ZS (1985) Diagnosis of Alzheimer's disease. Arch Neurol 42:1097–1105CrossRefGoogle Scholar
  15. Kumar S, Vijayan M, Bhatti JS, Reddy PH (2017) Chapter three-microRNAs as peripheral biomarkers in aging and age-related diseases. Prog Mol Biol Transl Sci 146:47–94CrossRefGoogle Scholar
  16. Lu M, Zhang Q, Deng M, Miao J, Guo Y, Gao W, Cui Q (2008) An analysis of human microRNA and disease associations. PLoS ONE 3:e3420CrossRefGoogle Scholar
  17. Ma X, Liu L, Meng J (2017) MicroRNA-125b promotes neurons cell apoptosis and Tau phosphorylation in Alzheimer’s disease. Neurosci Lett 661:57–62CrossRefGoogle Scholar
  18. Nelson PT, Wang WX, Rajeev BW (2008) MicroRNAs (miRNAs) in neurodegenerative diseases. Brain Pathol 18:130–138CrossRefGoogle Scholar
  19. Nunez-Iglesias J, Liu C-C, Morgan TE, Finch CE, Zhou XJ (2010) Joint genome-wide profiling of miRNA and mRNA expression in Alzheimer's disease cortex reveals altered miRNA regulation. PLoS ONE 5:e8898CrossRefGoogle Scholar
  20. Schonrock N, Ke YD, Humphreys D, Staufenbiel M, Ittner LM, Preiss T, Götz J (2010) Neuronal microRNA deregulation in response to Alzheimer's disease amyloid-β. PLoS ONE 5:e11070CrossRefGoogle Scholar
  21. Sun L, Jiang R, Li J, Wang B, Ma C, Lv Y, Mu N (2017) MicoRNA-425-5p is a potential prognostic biomarker for cervical cancer. Ann Clin Biochem 54:127–133CrossRefGoogle Scholar
  22. Wang X et al (2009) miR-34a, a microRNA up-regulated in a double transgenic mouse model of Alzheimer's disease, inhibits bcl2 translation. Brain Res Bull 80:268–273CrossRefGoogle Scholar
  23. Yu G, Li Y, Tian Q, Liu R, Wang Q, Wang J-Z, Wang X (2011) Berberine attenuates calyculin A-induced cytotoxicity and Tau hyperphosphorylation in HEK293 cells. J Alzheimer's Dis 24:525–535CrossRefGoogle Scholar
  24. Zhang Z, Li Y, Fan L, Zhao Q, Tan B, Li Z, Zang A (2015) microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo. Exp Therap Med 9:1617–1622CrossRefGoogle Scholar
  25. Zhang Y et al (2016) Micro RNA-425-5p regulates chemoresistance in colorectal cancer cells via regulation of Programmed Cell Death 10. J Cell Mol Med 20:360–369CrossRefGoogle Scholar
  26. Zhu H-C, Wang L-M, Wang M, Song B, Tan S, Teng J-F, Duan D-X (2012) MicroRNA-195 downregulates Alzheimer's disease amyloid-β production by targeting BACE1. Brain Res Bull 88:596–601CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Qingdao Mental Health CenterQingdaoPeople’s Republic of China

Personalised recommendations