Journal of Neural Transmission

, Volume 122, Issue 8, pp 1099–1103 | Cite as

Neuronal serine racemase regulates extracellular d-serine levels in the adult mouse hippocampus

  • Sayuri Ishiwata
  • Asami Umino
  • Darrick T. Balu
  • Joseph T. Coyle
  • Toru NishikawaEmail author
Translational Neurosciences - Short communication


In the hippocampus of mice lacking the gene for serine racemase (SR), a d-serine synthesizing enzyme, in the CaMKIIα-expressing neurons, we observed a significant decrease in the extracellular concentration of d-serine, a coagonist for the N-methyl-d-aspartate type glutamate receptor (NMDAR), and NMDAR hypofunction as revealed by diminished extracellular taurine concentrations after an intra-hippocampal NMDA infusion when compared to the wild type controls. Therefore, the neuronal SR could regulate the extracellular d-serine signaling responsible for NMDAR activation in the hippocampus.


CaMKIIα-expressing neurons Hippocampus In vivo microdialysis N-methyl-d-aspartate type glutamate receptor d-Serine Serine racemase 



This work was supported by the CREST (Core Research for Evolutional Science and Technology) program funded by the Ministry of Education, Culture, Sports, Science and Technology of Japan to TN research team including SI and AU. A postdoctoral National Research Service Award F32 MH090697 to DTB and grants R01MH05190 and P50MH0G0450 to JTC also supported this study. We thank the International Technology Exchange Society (an English editing agency: 3398 Tyler Drive Brunswick,OH 44212-3726 USA, Tel: 1-330-273-5409, Fax: 1-330-225-3834, E-mail: for their assistance in English editing for preparing our present manuscript, and are entirely responsible for the scientific content of this paper.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The present experiments were performed in accordance with the guidance of the Tokyo Medical and Dental University and were approved by the Animal Investigation Committee of the Institution.


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Sayuri Ishiwata
    • 1
  • Asami Umino
    • 1
  • Darrick T. Balu
    • 2
    • 3
  • Joseph T. Coyle
    • 2
    • 3
  • Toru Nishikawa
    • 1
    Email author
  1. 1.Department of Psychiatry and Behavioral Sciences, Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityBunkyo-kuJapan
  2. 2.Department of PsychiatryHarvard Medical SchoolBostonUSA
  3. 3.Laboratory for Psychiatric and Molecular NeuroscienceMcLean HospitalBelmontUSA

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