Journal of Neural Transmission

, Volume 121, Issue 10, pp 1281–1286 | Cite as

Automation of o-dianisidine assay for ceruloplasmin activity analyses: usefulness of investigation in Wilson’s disease and in hepatic encephalopathy

  • Mariacristina Siotto
  • Patrizio Pasqualetti
  • Massimo Marano
  • Rosanna Squitti
Neurology and Preclinical Neurological Studies - Original Article

Abstract

Ceruloplasmin (Cp) is a serum ferroxidase that plays an essential role in iron metabolism. It is routinely tested by immunoturbidimetric assays that quantify the concentration of the protein both in its active and inactive forms. Cp activity is generally analyzed manually; the process is time-consuming, has a limited repeatability, and is not suitable for a clinical setting. To overcome these inconveniences, we have set the automation of the o-dianisidine Cp activity assay on a Cobas Mira Plus apparatus. The automation was rapid and repeatable, and the data were provided in terms of IU/L. The assay was adapted for human sera and showed a good precision [coefficient of variation (CV) 3.7 %] and low limit of detection (LoD 11.58 IU/L). The simultaneous analysis of Cp concentration and activity in the same run allowed us to calculate the Cp-specific activity that provides a better index of the overall Cp status. To test the usefulness of this automation, we tested this assay on 104 healthy volunteers and 36 patients with Wilson’s disease, hepatic encephalopathy, and chronic liver disease. Cp activity and specific activity distinguished better patients between groups with respect to Cp concentration alone, and providing support for the clinical investigation of neurological diseases in which liver failure is one of the clinical hallmarks.

Keywords

Ceruloplasmin Ceruloplasmin activity Wilson’s disease Hepatic diseases Neurodegenerative diseases Hepatic encephalopathy 

Abbreviations

Cp

Ceruloplasmin

eCp

Enzymatic Cp

iCp

Immunologic Cp

CTRL

Healthy volunteers

WD

Wilson’s disease

CLD

Chronic liver disease

ENC

Hepatic encephalopathy

HCV

Hepatitis C virus

CV

Coefficient of variation

LoD

Limit of detection

LoB

Limit of blank

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Mariacristina Siotto
    • 1
  • Patrizio Pasqualetti
    • 2
    • 3
  • Massimo Marano
    • 4
  • Rosanna Squitti
    • 5
    • 6
  1. 1.Don Carlo Gnocchi Foundation ONLUSMilanItaly
  2. 2.Medical Statistics and Information Technology, Fatebenefratelli Foundation for Health Research and Education, AFaR DivisionRomeItaly
  3. 3.Unit of Clinical and Molecular EpidemiologyIRCCS “San Raffaele Pisana”RomeItaly
  4. 4.Department of NeurologyUniversity “Campus Bio-medico”RomeItaly
  5. 5.Fatebenefratelli Foundation for Health Research and Education, AFaR DivisionRomeItaly
  6. 6.Laboratorio di NeurodegenerazioneIRCCS “San Raffaele Pisana”RomeItaly

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