Journal of Neural Transmission

, Volume 120, Issue 8, pp 1217–1223 | Cite as

Cerebroprotective potential of resveratrol through anti-oxidant and anti-inflammatory mechanisms in rats

  • Prabhakar Orsu
  • B. V. S. N. Murthy
  • Annapurna Akula
Neurology and Preclinical Neurological Studies - Original Article

Abstract

Oxidative stress and inflammation are two important pathological mechanisms involved in cerebral ischemia and reperfusion injury. In pathological conditions such as cerebral infarction, the free radical production is greater than that of elimination by endogenous anti-oxidant system, by this undesirable effect brain is highly injured. Resveratrol is reported to have anti-oxidant and anti-inflammatory, athero-protective activities. Therefore, the aim of the present study is to evaluate the therapeutic potential of resveratrol against cerebral infarction induced by ischemia and reperfusion injury in Wistar rats. Bi-common carotid occlusion followed by 4 h reperfusion model was used to induce cerebral infarction. Percent infarction, oxidative stress markers (malondialdehyde, catalase, superoxide dismutase) and inflammatory markers (myeloperoxidase, TNF-α, IL-6, ICAM-1 and IL-10) were measured. TNF-α, IL-6, IL-10, and intracellular adhesive molecule-I (ICAM-1) levels were quantified by enzyme-linked immunosorbent assay (ELISA). Resveratrol produced significant dose-dependent reduction in percent cerebral infarct volume. At resveratrol 20 mg/kg dose, there was a significant reduction in oxidative stress and inflammatory markers like malondialdehyde, TNF-α, IL-6, myeloperoxidase and ICAM-I and in contrast there was a significant increase in anti-oxidants and anti-inflammatory markers like superoxide dismutase, catalase and IL-10 levels. Resveratrol showed significant cerebroprotective action mediated by anti-oxidant and anti-inflammatory mechanisms.

Keywords

Resveratrol Ischemia–reperfusion Antioxidant Anti-inflammatory ICAM-I 

Notes

Conflict of interest

Not applicable.

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Prabhakar Orsu
    • 1
  • B. V. S. N. Murthy
    • 2
  • Annapurna Akula
    • 3
  1. 1.Srinivasarao College of PharmacyVisakhapatnamIndia
  2. 2.Govt. Polytechnic College for WomenSrikakulamIndia
  3. 3.A.U. College of Pharmaceutical Sciences, Andhra UniversityVisakhapatnamIndia

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