NIR-labeled nanoparticles engineered for brain targeting: in vivo optical imaging application and fluorescent microscopy evidences
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The presence of the blood–brain barrier (BBB) makes extremely difficult to develop efficacious strategies for targeting contrast agents and delivering drugs inside the Central Nervous System (CNS). To overcome this drawback, several kinds of CNS-targeted nanoparticles (NPs) have been developed. In particular, we proposed poly-lactide-co-glycolide (PLGA) NPs engineered with a simil-opioid glycopeptide (g7), which have already proved to be a promising tool for achieving a successful brain targeting after i.v. administration in rats. In order to obtain CNS-targeted NPs to use for in vivo imaging, we synthesized and administrated in mice PLGA NPs with double coverage: near-infrared (NIR) probe (DY-675) and g 7. The optical imaging clearly showed a brain localization of these novel NPs. Thus, a novel kind of NIR-labeled NPs were obtained, providing a new, in vivo detectable nanotechnology tool. Besides, the confocal and fluorescence microscopy evidences allowed to further confirm the ability of g 7 to promote not only the rat, but also the mouse BBB crossing.
KeywordsNanoparticles NIR Optical imaging Confocal and fluorescence microscopy Brain targeting
The authors thank Luca Fabiani for microtome cut, Dr. Stefania Bettelli for the help in DAPI staining and Andrea Lorenzon for animal preparation. We also thank Prof. Luca Costantino for the synthesis of g7-PLGA polymer and Dr. Andrea Tombesi, CIGS (Centro Interdipartimentale Grandi Strumenti) of the University of Modena and Reggio Emilia for assistance in confocal analysis. This work is supported by Azzurra, Associazione Malattie Rare O.N.L.U.S.
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