Astrocytes and Glioblastoma cells release exosomes carrying mtDNA
- 3.7k Downloads
Cells can exchange information not only by means of chemical and/or electrical signals, but also via microvesicles released into the intercellular space. The present paper, for the first time, provides evidence that Glioblastoma and Astrocyte cells release microvesicles, which carry mitochondrial DNA (mtDNA). These microvesicles have been characterised as exosomes in view of the presence of some protein markers of exosomes, such as Tsg101, CD9 and Alix. Thus, the important finding has been obtained that bonafide exosomes, constitutively released by Glioblastoma cells and Astrocytes, can carry mtDNA, which can be, therefore, transferred between cells. This datum may help the understanding of some diseases due to mitochondrial alterations.
KeywordsGlioblastoma cells Astrocytes Mitochondrial DNA Exosomes Intercellular communication
- Agnati LF, Guidolin D, Baluska F, Leo G, Barlow PW, Carone C, Genedani S (2009) A new hypothesis of pathogenesis based on the divorce between mitochondria and their host cells: possible relevances for the Alzheimer’s disease. Curr Alzheimer Res (in press)Google Scholar
- Mallegol J, Van Niel G, Lebreton C, Lepelletier Y, Candalh C, Dugave C, Heath JK, Raposo G, Cerf-Bensussan N, Heyman M (2007) T84-intestinal epithelial exosomes bear MHC class II/peptide complexes potentiating antigen presentation by dendritic cells. Gastroenterology 132:1866–1876CrossRefPubMedGoogle Scholar
- Simons M, Raposo G (2009) Exosomes—vesicular carriers for intercellular communication. Curr Opin Cell Biol May 11. [Epub ahead of print]Google Scholar
- Skog J, Wurdinger T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, Curry WT Jr, Carter BS, Krichevsky AM, Breakefield XO (2008) Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10:1470–1476CrossRefPubMedGoogle Scholar