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Journal of Neural Transmission

, Volume 115, Issue 12, pp 1629–1642 | Cite as

Non-coding RNA as a trigger of neuropathologic disorder phenotypes in transgenic Drosophila

  • Elena Savvateeva-Popova
  • Andrej Popov
  • Abraham Grossman
  • Ekaterina Nikitina
  • Anna Medvedeva
  • Dmitry Molotkov
  • Nicholas Kamyshev
  • Konstantin Pyatkov
  • Olga Zatsepina
  • Natalya Schostak
  • Elena Zelentsova
  • Galina Pavlova
  • Dmitry Panteleev
  • Peter Riederer
  • Michail Evgen`ev
Basic Neurosciences, Genetics and Immunology - Original Article

Abstract

At most, many protein-misfolding diseases develop as environmentally induced sporadic disorders. Recent studies indicate that the dynamic interplay between a wide repertoire of noncoding RNAs and the environment play an important role in brain development and pathogenesis of brain disorders. To elucidate this new issue, novel animal models which reproduce the most prominent disease manifestations are required. For this, transgenic Drosophila strains were constructed to express small highly structured, non-coding RNA under control of a heat shock promoter. Expression of the RNA induced formation of intracellular aggregates revealed by Thioflafin T in embryonic cell culture and Congo Red in the brain of transgenic flies. Also, this strongly perturbed the brain control of locomotion monitored by the parameters of sound production and memory retention of young 5-day-old males. This novel model demonstrates that expression of non-coding RNA alone is sufficient to trigger neuropathology.

Keywords

Drosophila transgenic strains Non-coding RNA Intracellular aggregates Visuo-spatial orientation Locomotion Cognitive impairments 

Notes

Acknowledgments

The study was supported by Q-RNA Firm 799 (USA) and Grants of Russian Foundation for Fundamental Research 800 (E. S–P, A. P, M.E, O.Z) and Grant Molecular and Cellular Biology to M.E.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Elena Savvateeva-Popova
    • 1
  • Andrej Popov
    • 2
  • Abraham Grossman
    • 3
  • Ekaterina Nikitina
    • 1
  • Anna Medvedeva
    • 1
  • Dmitry Molotkov
    • 1
  • Nicholas Kamyshev
    • 1
  • Konstantin Pyatkov
    • 4
  • Olga Zatsepina
    • 5
  • Natalya Schostak
    • 5
  • Elena Zelentsova
    • 5
  • Galina Pavlova
    • 6
  • Dmitry Panteleev
    • 6
  • Peter Riederer
    • 7
  • Michail Evgen`ev
    • 5
  1. 1.Pavlov Institute of PhysiologyRussian Academy of SciencesSt PetersburgRussia
  2. 2.Sechenov Institute of Evolutionary Physiology and BiochemistrySt PetersburgRussia
  3. 3.MNESIS Company LLCPleasantvilleUSA
  4. 4.Division of BiologyCalifornia Institute of TechnologyPassadenaUSA
  5. 5.Engelhardt Institute of Molecular BiologyMoscowRussia
  6. 6.Institute of Gene BiologyMoscowRussia
  7. 7.Institute of Clinical Neurochemistry and National Parkinson Foundation Centre of Excellence Laboratories, Clinic and Policlinic for Psychiatry and PsychotherapyUniversity of WürzburgWürzburgGermany

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