Journal of Neural Transmission

, Volume 113, Issue 11, pp 1771–1778 | Cite as

CSF diagnosis of Alzheimer’s disease and dementia with Lewy bodies

  • M. Bibl
  • B. Mollenhauer
  • H. Esselmann
  • P. Lewczuk
  • C. Trenkwalder
  • P. Brechlin
  • E. Rüther
  • J. Kornhuber
  • M. Otto
  • J. Wiltfang


Differential diagnosis of Alzheimer’s disease (AD) and dementia with Lewy bodies (DLB) is often crucial. CSF Tau protein and Amyloid-beta (Aβ) peptides have shown diagnostic value for the diagnosis of AD, but discrimination from DLB was poor.

Herein, we investigate CSF of 18 patients with probable AD, 25 with probable DLB and 14 non-demented disease controls (NDC) by Aβ-SDS-PAGE/immunoblot and commercially available ELISAs for Aβ1-42 and tau. CSF Aβ peptide patterns and tau exhibited disease specific alterations among AD and DLB. The ratio of Aβ1-42 to Aβ1-38 and Aβ1-42 to Aβ1-37, respectively, in combination with absolute tau, yielded a sensitivity and specificity of 100 and 92%, respectively. We conclude that CSF Aβ peptide patterns and tau levels reflect disease-specific pathophysiological pathways of these dementias as distinct neurochemical phenotypes. Combined evaluation of these biomarkers provides a reasonable accuracy for differential diagnosis of AD and DLB.

Keywords: Alzheimer’s dementia, Lewy-body dementia, cerebrospinal fluid, amyloid-β peptides, tau protein, biomarker 


Aβ peptides

amyloid-beta peptides


amyloid-beta-sodium-dodecyl-sulphate-polyacrylamide-gel-electrophoresis with western immunoblot


Alzheimer’s disease

ApoE ɛ4

apolipoprotein E allele ɛ4


beta-amyloid precursor protein




charge coupled device camera


cerebrospinal fluid


carboxyterminally elongated


carboxyterminally truncated


dementia with Lewy bodies


enhanced chemiluminescence


Enzyme Linked Immunosorbent Assay


Mini-Mental-Status Examination


National Institute Neurological and Communicative Disorders and Stroke-Alzheimer’s Disease and Related Disorders Association




Parkinson’s disease dementia


polyvinylidene difluoride


sodium dodecyl sulphate


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • M. Bibl
    • 1
  • B. Mollenhauer
    • 2
  • H. Esselmann
    • 3
  • P. Lewczuk
    • 3
  • C. Trenkwalder
    • 4
  • P. Brechlin
    • 1
  • E. Rüther
    • 1
  • J. Kornhuber
    • 3
  • M. Otto
    • 5
  • J. Wiltfang
    • 3
  1. 1.Department of PsychiatryUniversity of GoettingenGoettingenGermany
  2. 2.Brigham and Women’s Hospital, Center for Neurologic DiseasesHarvard Medical SchoolBostonUSA
  3. 3.Department of Psychiatry and PsychotherapyUniversity of Erlangen-NurembergErlangenGermany
  4. 4.University of GoettingenParacelsus-Elena KlinikGermany
  5. 5.Department of NeurologyUniversity of UlmGermany

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