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Journal of Neural Transmission

, Volume 113, Issue 11, pp 1709–1715 | Cite as

TNF alpha affects the expression of GFAP and S100B: implications for Alzheimer’s disease

  • M. M. Edwards
  • S. R. Robinson
Article

Summary.

Neurodegenerative disorders such as Alzheimer’s disease are characterized by increased intracellular and extracellular concentrations of the astrocytic proteins glial fibrillary acidic protein (GFAP) and S100B. The present study examined the potential contribution of tumor necrosis factor alpha (TNFα) to these changes by measuring astrocyte viability along with the intracellular and extracellular expression of GFAP and S100B following exposure to this cytokine. Although TNFα did not affect astrocyte viability, the extracellular levels of both proteins were increased three-fold with associated reductions in immunocytochemical labeling.

Keywords: Astrocytes, TNFα, S100B, GFAP, Alzheimer’s disease 

Abbreviations:

AD

Alzheimer’s disease

AC

ammonium citrate

BSA

bovine serum albumin

DMEM

Dulbeccos Modified Eagle Medium

FBS

fetal bovine serum

GFAP

glial fibrillary acidic protein

LDH

lactate dehydrogenase

PB

phosphate buffer

PBS

phosphate buffered saline

TNFα

tumor necrosis factor alpha

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • M. M. Edwards
    • 1
  • S. R. Robinson
    • 1
  1. 1.School of Psychology, Psychiatry and Psychological Medicine, Monash UniversityClaytonAustralia

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