Regeneration of intervertebral discs in a rat disc degeneration model by implanted adipose-tissue-derived stromal cells
- 528 Downloads
Because adipose-tissue-derived stromal cell (ADSC) is readily accessible and abundant in stem cell, ADSC may be a better candidate for cell therapy and tissue engineering. This study investigated the potential of ADSC implantation to restore disc in a rat IVD model.
The first coccygeal disc segments of a Sprague–Dawley rat was left undamaged as a control (NC) group, and other two segments were damaged by needle injection. Two weeks later, ADSCs (TS) group or saline (IN) group was transplanted into each of the two damaged segments.
At 6 weeks after transplantation, the TS group showed a significantly smaller reduction in disc height than the IN group and exhibited a restoration of MRI signal intensity. Hematoxylin and eosin staining revealed a greater restoration of the inner annulus structure in the TS group. Anti-Human Nucleic Antibody, collagen type II, and aggrecan, staining showed positive findings at 2 weeks after transplantation in TS group.
ADSCs show potential for restoring degenerative discs and may prove effective in the treatment of IVD.
KeywordAdipose-tissue-derived stromal cell Animal model Intervertebral disc Degeneration
The Authors wish to thank Basic Science Research Program Through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number: 2009-0068925)
Conflict of interest statement
One or more of the authors Jeong has received funding from Basic Science Research Program through the National Research Foundation of Korea (NRF).
- 7.Jendelova P, Herynek V, Urdzikova L, Glogarova K, Kroupova J, Andersson B, Bryja V, Burian M, Hajek M, Sykova E (2004) Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. J Neurosci Res 76:232–243. doi: 10.1002/jnr.20041 CrossRefPubMedGoogle Scholar
- 14.Nishida K, Kang JD, Gilbertson LG, Moon SH, Suh JK, Vogt MT, Robbins PD, Evans CH (1999) Modulation of the biologic activity of the rabbit intervertebral disc by gene therapy: an in vivo study of adenovirus-mediated transfer of the human transforming growth factor beta 1 encoding gene. Spine 24:2419–2425CrossRefPubMedGoogle Scholar
- 17.Sakai D, Mochida J, Yamamoto Y, Nomura T, Okuma M, Nishimura K, Nakai T, Ando K, Hotta T (2003) Transplantation of mesenchymal stem cells embedded in Atelocollagen gel to the intervertebral disc: a potential therapeutic model for disc degeneration. Biomaterials 24:3531–3541.CrossRefPubMedGoogle Scholar
- 18.Sakai D, Mochida J, Iwashina T, Hiyama A, Omi H, Imai M, Nakai T, Ando K, Hotta T (2006) Regenerative effects of transplanting mesenchymal stem cells embedded in atelocollagen to the degenerated intervertebral disc. Biomaterials 27:335–345. doi: 10.1016/j.biomaterials.2005.06.038 CrossRefPubMedGoogle Scholar
- 20.Tapp H, Deepe R, Ingram JA, Kuremsky M, Hanley EN Jr, Gruber HE (2008) Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix. Arthritis Res Ther 10:R89. doi: 10.1186/ar2473 CrossRefPubMedGoogle Scholar