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Pollen morphology applied to species delimitation of Myrsine (Myrsinoideae-Primulaceae)

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Abstract

Myrsine, a genus in the family Primulaceae, includes closely related species whose taxonomic identification is difficult. Morphological analysis of pollen grains has provided key information for the classification of Primulaceae, helping clarify the taxonomy of several genera, subgenera, and species. However, a detailed study of pollen morphology in Myrsine was still lacking. Therefore, in this study, we investigated the potential of pollen characters to be used for the specific delimitation of Myrsine. Acetolyzed pollen grains were examined using light microscopy and non-acetolyzed pollen grains were analyzed by scanning electron microscopy. Pollen grains and monads of all analyzed species were isopolar, small to medium in size, and prolate spheroidal, subprolate, or prolate in shape, with (3)-4-colporate apertures, regular colpi or loxocolpi, and reticulate-granulate, granulate, rugulate-granulate, or microreticulate exine. Species considered difficult to identify based on other morphological traits were successfully distinguished using quantitative and qualitative pollen characters, confirming the importance of pollen morphology as a source of taxonomic information. Also, Myrsine stands out among genera of the subfamily Myrsinoideae because it shows great interspecific pollen variation, including two class sizes of pollen grains, three types of exine ornamentation, and exclusive loxocolporate pollen with inconspicuous endoapertures. This is the first report of loxocolporate pollen in Primulaceae.

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Acknowledgements

We are grateful to the Espírito Santo Research Foundation (FAPES) for funding the research on Primulaceae of T.T. Carrijo (grants no. 61860808/2013 and no. 222/2019), and to the Brazilian National Council for Scientific and Technological Development (CNPq) for the research fellowships awarded (bolsa de produtividade em pesquisa) to V. Gonçalves-Esteves, C.B.F. Mendonça, and T.T. Carrijo.

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Correspondence to Vania Gonçalves-Esteves.

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Appendix

Appendix

Myrsine altomontana M.F.Freitas & Kin.-Gouv. - Brasil. Paraná, Município de Guaratuba, Serra de Araçatuba-Morro dos Perdidos, *E.P. Santos 475, 12 May 1998 (RB); Santa Catarina, Município de Nova Trento, Prima Luna/Monte Barão, T.J. Cadorin 2640, 15 Jun 2010 (RB); Paraná, Município de Guaratuba, Serra de Araçatuba-Morro dos Perdidos, E.P. Santos 483, 12 May 1998 (RB).

Myrsine balansae (Mez) Otegui - Brasil. Paraná, Município de Tibagi, Fazenda Batavo, Rio Iapó, * M.C. Dias n.n., 7 Apr 1997 (RB345656).

Myrsine congesta (Schwacke ex Mez) Pipoly - Brasil. Rio de Janeiro, Município de Santa Maria Madalena, Alto do Desengano, *J.S. Lima 249, Aug 1934 (RB).

Myrsine coriacea (Sw.) R.Br. - Brasil. Bahia. Prado, Parque Nacional do Descobrimento, *F.B. Matos 1653, 10 Jul 2009 (RB).

Myrsine emarginella Miq. - Brasil. Rio de Janeiro, Município de Santa Maria Madalena, *Santos-Lima 340, Apr 1936 (RB).

Myrsine gardneriana A.DC. - Brasil. Paraná, Município de Pien, Lajeado Grande, *G. Hatschbach 78041, 15 Oct 2004 (RB); Minas Gerais, Município de Santo Antônio do Itambé, subida ao Pico do Itambé, A. Furlan n.n., 5 Apr 1982 (RB382734).

Myrsine glazioviana Warm. - Brasil. Minas Gerais, Parque Estadual Serra do Papagaio, próximo ao bairro Capão Redondo, *L. Echternacht 1527, 9 Nov 2007 (RB).

Myrsine guianensis (Aubl.) Kuntze - Brasil. Bahia. Salvador, Bosque Suiço, *J.G. Jardim 1126, 28 Sep 1997 (RB).

Myrsine lancifolia Mart. - Brasil. Paraná, galeria aberta sobre o rio Taquara, 5 km da Colônia de Castrolanda, *J.C. Lindeman 3004, 14 Oct 1966 (RB); Espírito Santo, Município de Santa Teresa, Nova Lombardia, Reserva Biológica Augusto Ruschi, Estrada para Lombardia, R.R. Vervolet 210, 25 Apr 2002 (RB).

Myrsine leuconeura Mart.- Brasil. Distrito Federal, Município de Planaltina, *Sousa-Silva 2003, 18 Jul 2000 (RB).

Myrsine lineata (Mez) Imkhan. - Brasil. São Paulo, Município de Santo André, Reserva Biológica do Alto da Serra de Paranapiacaba, *M. Sugiyama 1229, 29 Nov 1994 (RB); Rio de Janeiro, Município de Itatiaia, Caminho para Rio Bonito, A.C. Brade 18799, 2 Feb 1948 (RB).

Myrsine loefgrenii (Mez.) Imkhan. - Brasil. Paraná, Município de Irati, *W. Maschio 490, 6 Oct 2004 (RB); Paraná, Município de Castro, Rio Iapó, A.C. Cervi 2974, 1 Nov 1989 (RB).

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Myrsine parvifolia A.DC. - Brasil. Rio de Janeiro, Restinga de Maricá, *A. Arruda 3, 6 April 2009 (R); Santa Catarina, Município de Palhoça, praia de Massimbá, L.B. Smith, R. Reitz & R. Kleim 7258, 2 Nov 1956 (R); Bahia, Santa Cruz de Cabrália, R.M. Harley 17135, 18 Mar 1974 (RB).

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Myrsine squarrosa (Mez) M.F.Freitas & Kin.- Gouv. - Brasil. Minas Gerais, Município de Sacramento, *J.N. Nakajima 776, 11 Dec 1994 (RB).

Myrsine venosa A.DC. - Brasil. Rio de Janeiro, Alto da Boa Vista, *A.P. Duarte 5241, Jun 1960 (RB).

Myrsine villosissima Mart. - Brasil. Rio de Janeiro, Município de Itatiaia, Parque Nacional de Itatiaia, *J.M.A. Braga 4516, 3 Dec 1997 (RB); Minas Gerais, Município de Ouro Preto, M. Peron 80, 24 Feb 1987 (RB); Rio de Janeiro, Município de Santa Maria Madalena, A.C. Brade 1423, 3 Mar 1935 (RB).

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da Silva-Fourny, A.C., Hollunder, R.K., Garbin, M.L. et al. Pollen morphology applied to species delimitation of Myrsine (Myrsinoideae-Primulaceae). Plant Syst Evol 306, 21 (2020). https://doi.org/10.1007/s00606-020-01654-y

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Keywords

  • Myrsine
  • Palynology
  • Rapanea
  • Taxonomy