Genetic diversity in common wheat lines revealed by fluorescence in situ hybridization

  • Jun Guo
  • Dan Gao
  • Wenping Gong
  • Haosheng Li
  • Jianbo Li
  • Guangrong Li
  • Jianmin Song
  • Jianjun Liu
  • Zujun YangEmail author
  • Cheng LiuEmail author
Original Article


Molecular markers and phenotyping have been widely used to evaluate wheat germplasm diversity. However, the feasibility of using chromosome fluorescence in situ hybridization (FISH) to evaluate wheat genetic diversity has not been well investigated. In this study, seventy-six representative Chinese wheat lines in main wheat production area were selected and investigated with multicolour FISH using Oligo-pTa535, Oligo-pSc119.2 and Oligo-(GAA)8 probes. The results indicated that wheat chromosomes can be clearly recognized by FISH. For wheat A, B and D genomes, the number of FISH types ranged from 2 to 7, 2 to 6 and 1 to 5, respectively. The average number of FISH types in the A and B genomes was higher than that in the genome D. The rye-derived 1RS chromosome in wheat background could also be clearly detected by these probes. The frequency of 1RS in Chinese wheat lines investigated was 48.7%, and most (94.6%) of them belonged to 1BL.1RS. The genetic relationships among the seventy-six Chinese wheat lines subjected to FISH were divided into three clusters, e.g., CL1, CL2 and CL3. Those wheat lines derived from Shandong and Henan Provinces were mainly located in clusters CL1 and CL3, respectively, which may suggest that the FISH type is associated with the adaptation of wheat. These results also indicated that multicolour FISH using a combination of three different oligo-probes generates sufficiently diverse hybridization patterns among wheat lines to evaluate the genetic diversity of wheat.


Fluorescence in situ hybridization (FISH) Genetic analysis Triticum aestivum 



This research was funded by National Key Research and Development Program (2017YFD0100600 and 2016YFD0102000), Natural Science Foundation of Shandong Province (ZR2017MC004), the Modern Agricultural Industry Technology System and Agricultural scientific and technological innovation project of Shandong Academy of Agricultural Sciences (CXGC2018E01).

Author Contributions

C. Liu and Z. Yang conceived and designed the experiments. G. Dan, W. Gong, G. Li and J. Li performed the experiments. J. Guo, W. Gong, C. Liu and Z. Yang analysed the data. W. Gong, H. Li, J. Song, J. Guo and J. Liu contributed reagents/materials/analysis tools. J. Guo and C. Liu wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

606_2019_1567_MOESM1_ESM.xls (36 kb)
Supplementary material (XLS 35 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Jun Guo
    • 1
  • Dan Gao
    • 2
  • Wenping Gong
    • 1
  • Haosheng Li
    • 1
  • Jianbo Li
    • 2
  • Guangrong Li
    • 2
  • Jianmin Song
    • 1
  • Jianjun Liu
    • 1
  • Zujun Yang
    • 2
    Email author
  • Cheng Liu
    • 1
    • 3
    • 4
    Email author
  1. 1.National Engineering Laboratory for Wheat and Maize and Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley, Ministry of Agriculture, Crop Research InstituteShandong Academy of Agricultural SciencesJinanChina
  2. 2.School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduChina
  3. 3.College of Life ScienceShandong Normal UniversityJinanChina
  4. 4.Agronomy CollegeShandong Agricultural UniversityTai’anChina

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