Genetic diversity in common wheat lines revealed by fluorescence in situ hybridization
- 23 Downloads
Molecular markers and phenotyping have been widely used to evaluate wheat germplasm diversity. However, the feasibility of using chromosome fluorescence in situ hybridization (FISH) to evaluate wheat genetic diversity has not been well investigated. In this study, seventy-six representative Chinese wheat lines in main wheat production area were selected and investigated with multicolour FISH using Oligo-pTa535, Oligo-pSc119.2 and Oligo-(GAA)8 probes. The results indicated that wheat chromosomes can be clearly recognized by FISH. For wheat A, B and D genomes, the number of FISH types ranged from 2 to 7, 2 to 6 and 1 to 5, respectively. The average number of FISH types in the A and B genomes was higher than that in the genome D. The rye-derived 1RS chromosome in wheat background could also be clearly detected by these probes. The frequency of 1RS in Chinese wheat lines investigated was 48.7%, and most (94.6%) of them belonged to 1BL.1RS. The genetic relationships among the seventy-six Chinese wheat lines subjected to FISH were divided into three clusters, e.g., CL1, CL2 and CL3. Those wheat lines derived from Shandong and Henan Provinces were mainly located in clusters CL1 and CL3, respectively, which may suggest that the FISH type is associated with the adaptation of wheat. These results also indicated that multicolour FISH using a combination of three different oligo-probes generates sufficiently diverse hybridization patterns among wheat lines to evaluate the genetic diversity of wheat.
KeywordsFluorescence in situ hybridization (FISH) Genetic analysis Triticum aestivum
This research was funded by National Key Research and Development Program (2017YFD0100600 and 2016YFD0102000), Natural Science Foundation of Shandong Province (ZR2017MC004), the Modern Agricultural Industry Technology System and Agricultural scientific and technological innovation project of Shandong Academy of Agricultural Sciences (CXGC2018E01).
C. Liu and Z. Yang conceived and designed the experiments. G. Dan, W. Gong, G. Li and J. Li performed the experiments. J. Guo, W. Gong, C. Liu and Z. Yang analysed the data. W. Gong, H. Li, J. Song, J. Guo and J. Liu contributed reagents/materials/analysis tools. J. Guo and C. Liu wrote the paper.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights
This article does not contain any studies with human participants or animals performed by any of the authors.
- Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420. https://doi.org/10.1007/s00122-006-0365-4 CrossRefPubMedGoogle Scholar
- Anugrahwati DR, Shepherd KW, Verlin DC, Zhang P, Mirzaqhaderi G, Walker E, Francki MG, Dundas IS (2008) Isolation of wheat-rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin. Genome 51:341–349. https://doi.org/10.1139/G08-019 CrossRefPubMedGoogle Scholar
- Autrique E, Nachit M, Monneveux P, Tanksley SD, Sorrells ME (1996) Genetic diversity in durum wheat based on RFLPs, morphophysiological traits, and coefficient of parentage. Crop Sci 36:735–742. https://doi.org/10.2135/cropsci1996.0011183X003600030036x CrossRefGoogle Scholar
- Braun HJ, Atlin G, Payne T, Reynolds MP (2010) Multi-location testing as a tool to identify plant response to global climate change. Eur J Neurosci 23:1129–1141Google Scholar
- Chao S, Rouse MN, Acevedo M, Szabohever A, Bockelman H, Bonman JM, Elias E, Klindworth D, Xu S (2017) Evaluation of genetic diversity and host resistance to stem rust in USDA NSGC Durum Wheat Accessions. Pl Genome 10:1–13Google Scholar
- Chen J, Ren Z, Gao L, Jia J (2005) Developing new SSR markers from EST of wheat. Acta Agron Sin 31:154–158Google Scholar
- Cook JP, Blake NK, Heo HY, Martin JM, Weaver DK, Talbert LE (2017) Phenotypic and haplotype diversity among tetraploid and hexaploid wheat accessions with potentially novel insect resistance genes for wheat stem sawfly. Pl Genome 10(1):1–10Google Scholar
- Gong WP, Han R, Li HS, Song JM, Yan HF, Li GY, Liu AF, Cao XY, Guo J, Zhai SN, Cheng DG, Zhao ZD, Liu C, Liu JJ (2017) Agronomic traits and molecular marker identification of wheat-Aegilops caudata addition lines. Frontiers Pl Sci 8:1743. https://doi.org/10.3389/fpls.2017.01743 CrossRefPubMedPubMedCentralGoogle Scholar
- Guo J, Zhang X, Hou Y, Cai J, Shen X, Zhou T, Xu H, Ohm HW, Wang H, Li A (2015b) High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet 128:2301–2316. https://doi.org/10.1007/s00122-015-2586-x CrossRefPubMedGoogle Scholar
- Howell T, Hale I, Jankuloski L, Bonafede M, Gilbert M, Dubcovsky J (2014) Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status. Theor Appl Genet 127:2695–2709. https://doi.org/10.1007/s00122-014-2408-6 CrossRefPubMedPubMedCentralGoogle Scholar
- Liu JJ, Zhong-Hu HE, Peña JR, Zhao ZD (2004) Effect of 1BL/1RS translocation on grain quality and noodle quality in bread wheat. Acta Agron Sin 30:149–153Google Scholar
- Liu C, Li GR, Gong WP, Li GY, Han R, Li HS, Song JM, Liu AF, Cao XY, Chu XS, Yang ZJ, Huang CY, Zhao ZD, Liu JJ (2015) Molecular and cytogenetic characterization of a powdery mildew-resistant wheat-Aegilops mutica partial amphiploid and addition line. Cytogenet Genome Res 147:186–194. https://doi.org/10.1159/000443625 CrossRefPubMedGoogle Scholar
- Mago R, Spielmeyer W, Lawrence J, Lagudah S, Ellis G, Pryor A (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet 104:1317–1324. https://doi.org/10.1007/s00122-002-0879-3 CrossRefPubMedGoogle Scholar
- Qi Z, Liu D, Chen P, Li Q (2001) Molecular cytogenetic analysis of winter wheat germplasm aimengniu. Acta Bot Sin 43:469–474Google Scholar
- Quraishi UM, Abrouk M, Bolot S, Pont C, Throude M, Guilhot N, Confolent C, Bortolini F, Praud S, Murigneux A (2009) Genomics in cereals: from genome-wide conserved orthologous set (COS) sequences to candidate genes for trait dissection. Funct Integr Genomics 9:473–484. https://doi.org/10.1007/s10142-009-0129-8 CrossRefPubMedGoogle Scholar
- Villareal RL, Edel T, Mujeebkazi A, Rajaram S (1995) The 1BL/1RS chromosome translocation effect on yield characteristics in a Triticum aestivum L. cross. Pl Breed 114:497–500. https://doi.org/10.1111/j.1439-0523.1995.tb00843.x CrossRefGoogle Scholar
- Yan BJ, Zhang HQ, Ren ZL (2005) Molecular cytogenetic identification of a new 1RS/1BL translocation line with Secalin absence. Hereditas (Beijing) 27:513–517Google Scholar