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Plant Systematics and Evolution

, Volume 305, Issue 8, pp 597–609 | Cite as

Morpho-anatomical differentiation and genome size variation in three ploidy levels within the B7 cytotype of Prospero autumnale (Hyacinthaceae) complex from the Balkan Peninsula and Pannonian Basin

  • Ana Vestek
  • Marek SlovákEmail author
  • Hanna Weiss-Schneeweiss
  • Eva M. Temsch
  • Jadranka Luković
  • Jaromír Kučera
  • Goran Anačkov
Original Article
  • 330 Downloads
Part of the following topical collections:
  1. Plants of the Balkan Peninsula in Space and Time

Abstract

Prospero autumnale (Hyacinthaceae) is taxonomically intricate geophyte species complex with a circum-Mediterranean distribution. In contrast to the extraordinary karyological diversity within P. autumnale complex, morphological variation is limited, and thus, reliable taxonomically diagnostic traits are absent. This investigation addresses whether different ploidy levels within cytotype B7 can be distinguished morpho-anatomically. Populations of B7 cytotype from the Balkan Peninsula and Pannonian Basin were examined. Karyological variation was analyzed, and genome size measurements via flow cytometry were taken for selected individuals from each population. Morpho-anatomical analyses were based on 29 anatomical and 25 morphological traits. Multivariate morphometric analyses revealed significant differences between the three ploidy levels, and this may assist in the identification of plant ploidy level. Although the diploids were the most distinct group, some differences were also found between tetraploids and hexaploids. Genome size variation was found within diploids and tetraploids, and some downsizing was observed in hexaploids. This is the first evaluation of morpho-anatomical variation of cytotype B7 in the P. autumnale complex using a multivariate morphometric approach; further taxonomic and evolutionary studies in this taxonomically complex group are needed.

Keywords

Balkans Genome size Multivariate morphometrics Ploidy levels Prospero autumnale 

Notes

Acknowledgements

This study was funded by the Ministry of Education, Science and Technological Development of Republic of Serbia (Project No. 173030) and by the Austrian Federal Ministry of Education, Science and Research (BMBWF), the Austrian Agency for International Mobility and Cooperation in Education, Science and Research (OeAD-GmbH) and the Centre for International Cooperation and Mobility (ICM) (Ernst Mach Grant program). The authors would like to thank Norbert Bauer (Hungarian Natural History Museum, Budapest), Bojana Bokić, Jelena Knežević and Boris Radak (University of Novi Sad, Faculty of Sciences) for the assistance in field work and Bojan Zlatković (University of Niš, Faculty of Sciences and Mathematics) for providing information on localities of P. autumnale in southern Serbia. We are also deeply thankful to Professor John Parker (University of Cambridge) for language improvements. For valuable comments of the two anonymous reviewers, the authors are appreciative.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Authors and Affiliations

  1. 1.Department of Biology and Ecology, Faculty of SciencesUniversity of Novi SadNovi SadSerbia
  2. 2.Plant Sciences and Biodiversity CenterSlovak Academy of SciencesBratislavaSlovak Republic
  3. 3.Department of BotanyCharles UniversityPragueCzech Republic
  4. 4.Department of Botany and Biodiversity ResearchUniversity of ViennaViennaAustria

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