Comparative pollen–pistil interactions and insect pollination in two Hypoxis species (Hypoxidaceae) in China and North America
- 198 Downloads
We compared the floral ecology and pollen–pistil interactions in Hypoxis hirsuta (L.) Coville from North America and Hypoxis aurea Loureiro from China. Both species are vernal-flowering herbs, with yellow perianths, providing pollen as their only reward. In H. hirsuta, hand self-pollinated, emasculated and bagged control flowers failed to set fruit. When cross-pollinated, 77% of the pistils produced seed-filled capsules while 72% of open, insect-pollinated flowers set seed. Epifluorescence showed that most germinating grains failed to penetrate the stigmatic surface > 24 h after hand self-pollination suggesting early-acting self-incompatibility (SI). The majority of pollinators of H. hirsuta were female bees representing 21 species distributed in four families. Pollen load analyses indicated that most bees were polylectic and carried grains of H. hirsuta mixed with pollen of co-blooming, nectariferous species. In contrast, capsule production of H. aurea was 60% for open, insect-pollinated flowers. Pollen tubes of H. aurea also entered ovules within 24 h, and SI of this species was partial. Evidence of a late-acting rejection in this species following self-pollination included abnormal growth of pollen tubes in styles with fewer tubes penetrating ovules. In contrast, abnormal tube growth was not observed in styles of H. hirsuta. Hypoxis aurea received fewer insect visitations. Pollen was dispersed primarily by hoverflies (Syrphidae) followed by bees. Floral presentation, generalist pollination and two modes of SI found in these two Hypoxis species contribute to our understanding of floral evolution for congeners isolated on different continents.
KeywordsBees Hoverflies Hypoxidaceae Hypoxis Self-incompatibility
We thank Dr. Peter E. Gibbs of the University of St Andrews, UK, for comments and discussions on self-incompatibility; Dr. Huan-Li Xu of China Agricultural University for identification of bee specimens collected on H. aurea and Dr. Kui-Yan Zhang of Institute of Zoology, Chinese Academy of Sciences for identification of hoverflies (Syrphidae). We also thank Ms. Dowen Jocson of St. Louis University for insects measurement. We thank four anonymous reviewers for their detailed comments for improving this manuscript. Dr. Zong-Xin Ren’s work at St. Louis University was funded by Chinese Academy of Sciences. Work of H. aurea was supported by the National Natural Science Foundation of China (No. 31300199) and the Youth Innovation Promotion Association, Chinese Academy of Sciences (2014355).
Compliance with ethical standards statement
Conflict of interest
We declare that there is no conflict of interest relative to the paper.
Human and animal rights
This work involved no human participants as subjects. Animal collection was restricted to a minimum and complied with laws.
All authors consent with the paper and are aware of its content and organization. As the corresponding author, I am prepared to provide further documents of compliance with ethical standards upon request along the editorial process.
- Bernhardt P (1996) Anther adaptations for animal pollination. In: D’Arcy W, Keating R (eds) The biology of anthers. Cambridge University Press, New York, pp 192–220Google Scholar
- Bernhardt P, Edens-Meier R, Vance N (2013) Pollination ecology and floral function of Brown’s peony (Paeonia brownii) in the Blue Mountains of northeastern Oregon. J Pollination Ecol 1:9–20Google Scholar
- Bernhardt P, Edens-Meier R, Westhus E, Vance N (2014) Bee-mediated pollen transfer in two populations of Cypripedium montanum Douglas ex Lindley. J Pollination Ecol 13:188–202Google Scholar
- Buchmann SL (1983) Buzz pollination in angiosperms. In: Jones CE, Little RJ (eds) Handbook of experimental pollination biology. Van Nostrand Reinhold, New York, pp 73–113Google Scholar
- Duman J, Horwath K (1983) The role of hemolymph proteins in the cold tolerance of insects. Annual Rev Physiol 45:261–270. https://doi.org/10.1146/annurev.ph.45.030183.001401 CrossRefPubMedGoogle Scholar
- Herndon A (2002) Hypoxis L. In: Flora of North America Editorial Committee (eds) Flora of North America, vol. 26. Magnoliophyta: Liliidae: Liliales and Orchidales. Oxford University Press, New York, pp 201–204Google Scholar
- Ji ZH, Meerow AW (2000) Amaryllidaceae. In: Wu ZY, Raven PH (eds) Flora of China, vol. 26. Science Press and Missouri Botanical Garden Press, BeijingGoogle Scholar
- Nordal I (1998) Hypoxidaceae. In: Kubitzki K (ed) The families and genera of vascular plants vol 3 flowering plants monocotyledons: Lilianae (except Orchidaceae). Springer, Berlin, pp 286–295Google Scholar
- Ollerton J, Liede-Schumann S, Endress ME, Meve U, Rech AR, Shuttleworth A, Keller HA, Fishbein M, Alvarado-Cárdenas LO, Amorim FW, Bernhardt P, Celep F, Chirango Y, Chiriboga-Arroyo F, Civeyrel L, Cocucci A, Cranmer L, da Silva-Batista IC, de Jager L, Deprá MS, Domingos-Melo A, Dvorsky C, Agostini K, Freitas L, Gaglianone MC, Galetto L, Gilbert M, González-Ramírez I, Gorostiague P, Goyder D, Hachuy-Filho L, Heiduk A, Howard A, Ionta G, Islas-Hernández SC, Johnson SD, Joubert L, Kaiser-Bunbury CN, Kephart S, Kidyoo A, Koptur S, Koschnitzke C, Lamborn E, Livshultz T, Machado IC, Marino S, Mema L, Mochizuki K, Morellato LPC, Mrisha CK, Muiruri EW, Nakahama N, Nascimento VT, Nuttman C, Oliveira PE, Peter CI, Punekar S, Rafferty N, Rapini A, Ren ZX, Rodríguez-Flores CI, Rosero L, Sakai S, Sazima M, Steenhuisen SL, Tan CW, Torres C, Trøjelsgaard K, Ushimaru A, Vieira MF, Wiemer AP, Yamashiro T, Nadia T, Queiroz J, Quirino Z (2018) The diversity and evolution of pollination systems in large plant clades: Apocynaceae as a case study. Ann Bot (Oxford) (Online First). https://doi.org/10.1093/aob/mcy127 CrossRefGoogle Scholar
- R Development Core Team (2012) R: A language and environment for statistical computing. R foundation for statistical computing, ViennaGoogle Scholar
- Raimundez E, Ramirez N (1998) Reproductive strategy of a perennial herb: Hypoxis decumbens (Hypoxidaceae). Revista Biol Trop 46:555–565Google Scholar
- Rudall PJ (2002) Unique floral structures and iterative evolutionary themes in asparagales: insights from a morphological cladistic analysis. Bot Rev 68:488–509. https://doi.org/10.1663/0006-8101(2002)068%5b0488:Ufsaie%5d2.0.Co;2 CrossRefGoogle Scholar