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Pollination biology and breeding system in five nocturnal species of Oenothera (Onagraceae): reproductive assurance and opportunities for outcrossing

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Abstract

The capacity to produce seed, both by selfing and outcrossing, or mixed mating strategies, is considered a mechanism for overcoming unpredictable pollinator availability. In the present study, we investigate breeding system, insect visitations and the role of insect visitors in the pollination of five species of Oenothera subsect. Oenothera. Field experiments showed that autonomous selfing occurs at bud stage, prior to the opening of the flower. Control flowers showed similar seed set to hand-pollinated flowers, whereas emasculated flowers and those subject to open pollination set fewer seed. These results are consistent with the hypothesis that the investigated Oenothera exhibit a great capacity for autonomous selfing and that selfing is selected in order to provide reproductive assurance. Although flowers were visited mostly by nocturnal lepidopterans, these insects did not precipitate pollination and are thus considered nectar thieves. Conversely, analysis of pollen loads and behavior during foraging by diurnal insect visitors revealed that honeybees and bumblebees are the probable pollinators. We conclude that production of flowers capable of autonomous selfing at bud stage, followed by anthesis and opportunities for outcrossing, probably improves the invasive potential of these Oenothera in Europe, together with a rapid increase in their populations, even when pollinators are scarce.

Introduction

Most angiosperms depend on animals for pollination, and zoogamy appears to be an essential aspect of successful flowering plant reproduction (Lloyd 1980; Charlesworth and Charlesworth 1987). Evolutionary shifts in flowering plants have led to various mating patterns (Cruden 1977), and the most prevalent trend in the evolution of angiosperm reproduction is a transition from outcross to self-pollination (Lloyd 1992; Charlesworth et al. 1990). Theoretically, such plants should evolve toward either complete self-fertilization or outcrossing (Lande and Schemske 1985; Charlesworth et al. 1990). Studies of selfing rates in natural populations, however, have indicated that mixed mating systems, which involve both selfing and outcrossing, may be more common than is predicted by models (Vogler and Kalisz 2001; Fornoni et al. 2004; Goodwillie et al. 2005). It is generally accepted that one of the most significant factors responsible for the maintenance of a mixed mating system within a population is natural selection for reproductive assurance (Darwin 1876; Baker 1955). The latter model involves strategies which confer fitness advantages that outweigh the disadvantages generally associated with inbreeding and reduced genetic diversity of progeny (Lande and Schemske 1985). Consequently, increased self-fertilization is favored in situations where outcrossing sexual reproduction is limited, for example owing to the scarcity of pollinators and/or mates, or intense competition between individual plants for the service of pollinators (Wolfe and Barrett 1988; Eckert 2002; Kalisz and Vogler 2003; Fenster et al. 2004; Kalisz et al. 2004; Munoz et al. 2015).

Mixed mating within a population may occur in a variety of ways and/or at different levels. For example, populations may comprise either self-compatible and/or self-incompatible individuals, resulting in a genetically based selfing rate polymorphism (Stone 2002). Besides this, species may also exhibit heteromorphic flower systems, for example cleistogamous (completely selfing) and chasmogamous flowers (involving both outcrossing and selfing, Masuda et al. 2004). The most common system, however, is where individuals possess a single type of flower, and therefore, their fruit contains selfed, outcrossed or a mixture of both progeny types (Schoen and Brown 1991). The selfing that occurs as a consequence of mixed mating may take place within individual flowers (autogamy) or between flowers (geitonogamy) on a particular plant, and within-flower selfing can be either autonomous or vector mediated (Goodwillie et al. 2005 and references therein). Furthermore, mixed mating may occur as a result of one of three modes of autonomous selfing, based on the stage at which outcross pollen is received, namely: prior (before anthesis), competing (during anthesis) and delayed (at late anthesis; Schoen and Brown 1991; Lloyd 1992).

The genus Oenothera L. (evening primroses), one of the most studied and complex groups of species in the Onagraceae, has long served as a model system that integrates ecology, evolution and genetics of species interactions (Raven 1988; Johnson 2011). Indeed, species of Oenothera have been widely used as experimental plants in studies involving self-incompatibility, chloroplast function, complex heterozygosity and interactions between the genome and plastome. Consequently, Oenothera has played an important role in the modern synthesis of evolutionary biology (Johnson 2011; Golczyk et al. 2014). Recent molecular phylogeny work has clarified relationships within Oenothera, in particular that of genera that were once segregated: Gaura, Calylophus and Stenosiphon (Wagner et al. 2007 and references therein). Studies of Oenothera have revealed considerable variation in pollination and breeding systems; i.e., some taxa are self-incompatible and outcrossed (e.g., O. greggii and O. albicaulis; Gregory 1964; Theiss et al. 2010), whereas others are self-compatible and autogamous (e.g., O. sessilis and O. simulans; Krakos and Fabricant 2014; Krakos et al. 2014). According to Raven (1979), hawkmoth pollination is predominant in Oenothera, whereas pollination by bees has been documented for sections Hartmannia and Kneiffia.

In the present study, our analyses focus on five species of Oenothera assigned to section Oenothera and subsection Oenothera (subsect. Oenothera hereafter), which differ from other taxa in terms of origin, European distribution and status in Poland (alien, native; sensu Rostański 1985 and Rostański et al. 2010). Previous studies of this subsection indicate that almost all species are self-compatible; however, both self-compatible and self-incompatible individuals have been documented for a population of O. grandiflora in Alabama (Stubbe and Raven 1979). The most prominent and typical characteristic of subsect. Oenothera is the evolution of autogamy (Dietrich et al. 1997). Thus, based on the classification of breeding systems proposed by Cruden (1977), these species can be considered ‘obligately autogamous.’ According to Raven (1979), a reduction in flower parts and in the quantities of floral rewards offered has occurred in highly autogamous Oenothera as a consequence of shifts from energy-rich pollination systems to less energy-rich systems. More recently, however, it has been demonstrated that several species of subsect. Oenothera occurring in Poland produce copious amounts of floral nectar (up to 26 mg/flower), and nectar production has been related to the preferences of floral insect visitors observed in the field (Antoń et al. 2017). Although many papers have been published on members of subsect. Oenothera, detailed studies of pollination and reproductive biology have, in fact, been conducted only on O. elata in South America (Gregory 1964) and O. glazioviana in Japan (Kawano et al. 1995). These showed that both species are mostly hawkmoth pollinated. Even so, a substantial amount of autogamy has also been observed. By contrast, a preliminary survey of floral visitors in members of subsect. Oenothera in southeastern Poland (Antoń 2015; Antoń et al. 2017) revealed that hawkmoths do not visit these species. Instead, both nocturnal and diurnal insects (including moths and bees) frequently visit flowers of Oenothera, possibly indicating a more generalist pollination syndrome.

To the best of our knowledge, no detailed studies of the pollination biology and breeding systems of species assigned to subsect. Oenothera growing in Europe have been undertaken to date. As a result, these species present a great paradox—namely, that despite being as well known both genetically and biologically as almost any other group of plants, the study of much of their pollination and reproductive biology remains neglected. Given that much of the information available for species of subsect. Oenothera is contradictory, and given their significance as both crop plants (e.g., in the pharmacological industry; Bosisio 1990) and European invasive species (Mihulka et al. 2003; Tokhtar and Groshenko 2014; Rostański and Verloove 2015), an investigation of their pollination biology and breeding systems is long overdue. In order to address this, we employed four approaches, namely: (i) pollinator exclusion and hand-pollination experiments, (ii) emasculation experiments, (iii) spectrum and behavior analysis of floral visitors and (iv) identification of putative pollinator.

Materials and methods

Study site and plant species

The survey took place at the Botanical Garden of the Maria Curie-Skłodowska University in Lublin, SE Poland (51°15ʹ44ʹN, 22°30ʹ48ʹE), during the period 2013–2015. Five Oenothera species differing in their geographical distribution and status in Poland (sensu Rostański 1985; Rostański et al. 2010) were investigated. These were: O. casimiri Rostański, O. flaemingina Hudziok, O. nuda Renner ex Rostański, O. paradoxa Hudziok and O. rubricaulis Kleb. These Oenothera species are biennials, and therefore, each year, prior to investigations, experimental plants were established as follows. Seeds produced in 2012 from natural populations established in SE Poland (i.e., in Lublin and Mielec) were sown each year onto a light soil substrate at the end of April for the period 2012–2014. Subsequently, seedlings selected randomly were planted out each September in experimental plots (approx. 2 m2 in two replicates for each species). During the investigations, the plants were grown each year on loess soil at pH 6–7, at a site fully exposed to the sun.

The genus Oenothera currently comprises about 145 species assigned to 18 sections native to North, Central and South America. Of these, about 60 taxa have been reported to occur in the flora of Poland (Rostański and Tokarska-Guzik 1998; Rostański et al. 2004; Wagner et al. 2007; Rostański et al. 2010). Flowers of the investigated species produce both pollen and nectar as floral rewards. Floral nectar is exuded by secretory structures (nectaries) located at the base of a long, tubular hypanthium (Antoń et al. 2017). Pollen grains are connected to each other by viscin threads, and pollen is produced in eight large, elongated anthers that dehisce longitudinally (Szklanowska and Czubacki 2000; Antoń and Denisow 2018). Some species assigned to this subsection have recently been used as a pharmacological crop for the extraction of the fatty acid γ-linolenic acid from their seed (Bosisio 1990).

In addition, members of subsect. Oenothera (2n = 2x = 14), examined in the present paper, represent the best studied example to date of an uncommon and anomalous cytological phenomenon, namely permanent translocation heterozygosity (PTH; Raven 1979; Golczyk et al. 2014). The taxonomy of the genus Oenothera is very complicated, especially with regard to section Oenothera and subsection Oenothera, and there are at least two alternative taxonomical approaches, the first based mostly on cytogenetical studies (referred to as ‘American school’; e.g., Dietrich et al. 1997), and the second, population-based concept that takes into account Renner’s chromosomal complexes and ‘constant’ phenotypes (referred to as ‘European school’). The latter is supported by Professor Krzysztof Rostański and his co-workers (e.g., Rostański 1985; Rostański et al. 2010; Rostański and Verloove 2015). According to Rostański et al. (2004), the species concept of the ‘American school’ is misleading as it distinguishes only 13 species of Oenothera within subsect. Oenothera, each having a long list of synonyms, which, in fact, are true species in their own right, having their own characters and distribution. The last authors also claimed that within the ‘American school’ concept, the broadly treated Oenothera species represent a mixture of quite different taxa best treated as species sensu latissimo. Therefore, so as to adopt a more analytical approach, we favor the concept proposed by the ‘European school’ for the present paper.

Flowering and flower development

The onset and duration of the flowering period were recorded for all plants investigated in 2013–2015. In order to establish the length of anthesis, times of nectar production and pollen availability, we randomly marked buds (n = 15; per year/per species) approx. 3 days before anthesis. Then, twice a day, i.e., in the morning (c.a. 9:00) and evening (i.e., c.a. 19:00), we noted the progress of flowering and presentation of floral rewards (i.e., pollen and nectar). Since flowers of Oenothera appeared to release pollen at the bud stage (i.e., approx. 24–36 h before anthesis), buds damaged during observations of pollen availability (approx. 3–5 buds, per year/per species) were excluded from further analysis of floral longevity and nectar production.

Pollen/ovule ratio and pollen quality

In order to estimate the pollen to ovule ratio (P/O; Cruden 1977), the number of pollen grains per anther per flower, as well as the number of ovules, was estimated in 2014 for a single flower per plant. The flowers (n = 10) were collected from different individuals (n = 10 individuals per species) and fixed in FAA solution (formalin: acetic acid: ethanol at a ratio of 5:5:90, by volume) in individual vials. The number of ovules was estimated for ten flowers collected from different individuals (n = 10 individuals per species). In determining pollen counts, only mature, undehisced anthers were used for calculations as follows. Anthers were placed in disposable centrifuge tubes, macerated and extracted in 1 ml 70% ethanol. Following vortexing, 50 µl of suspension containing pollen grains was placed on microscope slides. A coverslip was added and pollen grains scored by means of macroscopic and microscopic observations performed using an Olympus SZX12 (Tokyo, Japan) stereomicroscope and Nikon Eclipse E200 light microscope, respectively. Six replicates were made for each pollen suspension sample. Four anthers per flower were analyzed in order to obtain the average value for a single anther. This was then multiplied by eight (the number of anthers per flower) in order to arrive at the mean number of pollen grains per flower.

The quality of pollen was assessed for 2013 and 2014 by testing for viability using a standard 2% (w/v) aqueous acetocarmine–glycerin solution (1:1) placed on microscope slides. Pollen was collected from ten flowers per species per year, and the observations were performed using a Nikon Eclipse E200 light microscope.

Stigma receptivity

The receptivity of stigmas was tested in the field using the Peroxtesmo Ko. (Merck) method (Dafni and Maues 1998). In 2013 and 2014, stigma receptivity was scored for flowers (n = 10 per species/per stage/per year) and three stages, i.e., (1) bud stage (2 days and 1 day, respectively, before anthesis), (2) at the onset and (3) at the end of anthesis.

Breeding system

The breeding system of the investigated populations of five Oenothera species was studied for the period 2013–2015. Every year, we randomly selected and marked 50 buds (per species; ca. 3 days before anthesis) on different individuals (n = 15); all pollination treatments were replicated within particular individuals. Also, in 2013, randomly chosen buds (n = 10 per species, from ten different individuals) were emasculated and enclosed within tulle isolator bags in order to test for the presence of apomixis. As a result, a total of 800 flowers were used during the course of 3 years of experiments. The flowers were divided into five groups and subjected to the following pollination treatments (n = 10 flowers for each treatment per year per species): (I) control—flowers left undisturbed for open pollination; (II) geitonogamy—buds (approx. 2 days before anthesis) carefully emasculated without disturbing floral development and before the anthers had dehisced, enclosed within tulle isolator bags (mesh size < 1 mm) and then hand-pollinated once the corolla had opened with pollen collected from the same individual; (III) spontaneous self-pollination—buds enclosed within tulle isolator bags to prevent insect visits; (IV) spontaneous cross-pollination—buds emasculated as above and then left uncovered; and (V) induced cross-pollination—buds emasculated as above, enclosed within tulle isolator bags and then manually pollinated with fresh pollen collected from a different individual. About 3–5 days following the end of anthesis, the flowers were unbagged and left in the experimental plots. Every year, potentially damaged flowers resulting from emasculation were excluded from further experiments. Despite the large number of flowers produced per inflorescence, it was essential for all investigated species that 3–5 flowers were open simultaneously on any given inflorescence, so as not to necessitate the emasculation of additional flowers not used in the experiments, thereby preventing uncontrolled pollen transfer from other flowers borne on the same inflorescence. Therefore, the results presented in this paper are an accurate indication of final seed production following all pollination treatments employed.

Capsule collection

Capsules were harvested 4–6 weeks following pollination and stored separately in paper envelopes until they could be analyzed. Damaged capsules, as well as ones showing signs of herbivore activity (e.g., holes or droppings), were excluded from further analyses. In the laboratory, the length of the fruit (to an accuracy of 0.1 mm) was measured. Capsules were then placed in a Petri dish, and the number of normally developed seeds calculated for each sample.

Flower visitor observations and identification

Observations of insect visitors to flowers were conducted during the peak flowering period (i.e., early July) for five separate days during 2013 and 2014 for all investigated Oenothera species. Owing to the specifically nocturnal anthesis of the species studied, observations began each day at approx. 19:00 and lasted throughout the night until 9:00–10:00 (GMT + 2 h). Thus, each day comprised 16 rounds of observations at 1 h intervals in the experimental area (approx. 1–2 m2 for each species). Each round of observations was performed by two people (for each species), lasted 10–15 min and comprised: (i) observation of all insect visits to an individual plant species in order to establish the spectrum of insect visitors/frequency, (ii) observations of insect behavior, (iii) measurement of the duration of insect visits, (iv) insect photography and (v) insect capture for further analysis of pollen loads. In periods of very strong wind or rain, observations were halted and completed at the corresponding time on the days that followed. Insect visitors were assigned to five groups: Lepidoptera, Bombus spp., Apis mellifera, Coleoptera and Diptera. Insect identifications were based on the available taxonomic keys or performed by the regional specialist entomologist.

In addition, in 2014, in order to undertake detailed observations of the specific behavior of nocturnal floral insects visiting the investigated Oenothera, video recording was employed. This was achieved by means of a digital video camera (GZ-E10RU, JVC Everio, JVC Kenwood Corp., Japan).

Pollen load analyses

For insect pollen load analyses, we used insects captured in 2013 and 2014 visiting the five investigated species of Oenothera. Insect visitors were collected with an entomological net and killed in a jar containing ethyl acetate vapor. Insects were then pinned and taken back to the laboratory for further quantitative analysis of the pollen that they carried. In the case of bees (i.e., Apis mellifera and Bombus spp.), we did not analyze corbicular loads, since this pollen is not accessible during pollination. In order to quantify the pollen grains carried by each visitor, we employed the method used by Zych (2007). Pollen samples were analyzed using a Nikon Eclipse E200 (Tokyo, Japan) light microscope, and the number of pollen grains of Oenothera and ‘other’ species calculated. In the case of large pollen loads (> 500), pollen grains were calculated from nine regions consistently present on the cover slip and then extrapolated.

Statistical analyses

Data are presented as mean values ± SD (standard deviation). When possible, parametric statistical analysis was applied. Post hoc comparison of means was tested using the HSD Tukey test. The Kruskal–Wallis test was used to obtain differences for non-normally distributed data. The Spearman correlation coefficient was used to test the relationship between capsule length and seed set for each investigated species. The level of statistical significance was P = 0.05. All statistical analyses were performed using Statistica 13.1 (Statsoft Poland, Cracow).

Results

Flowering and floral biology

The dates of flowering for all species are shown in Table 1. In general, the onset of flowering of investigated Oenothera species occurred in mid- or late June, and flowering lasted until late July and/or early August. Flowers of all species exhibited nocturnal anthesis; i.e., flowers of Oenothera opened specifically late in the evening, i.e., 20:00–21:00, and lasted until early morning, i.e., 7:00–9:00. The flowering time of a single flower was similar for all species examined (F4,142 = 2.456, P = 0.07; Fig. 1) and relatively short, lasting 10.3 ± 3 h (mean ± SD, calculated across 2 study years and species). Nectar production in all Oenothera species commenced at the bud stage, approx. 4–5 h before anthesis. Conversely, the release of pollen from anthers began much earlier than did nectar production, i.e., approx. 24 h before anthesis (O. flaemingina and O. nuda) and approx. 36 h before anthesis (O. casimiri, O. paradoxa, O. rubricaulis). Significant differences in the period of nectar production (F4,142 = 3.168, P = 0.032) and pollen availability (F4,142 = 5.343, P = 0.004) were observed for all species (Fig. 1; means calculated across 2 study years).

Table 1 Dates of flowering and details of pollen number, pollen/ovule ratio and pollen viability in the five Oenothera species
Fig. 1
figure1

Mean floral longevity, period of nectar production and pollen availability in five Oenothera species in Lublin, SE Poland. Data represent mean values (calculated across study years) ± SD (standard deviation). Means with the same lower case letter do not differ significantly between plant species at P < 0.05, based on the HSD Tukey test

The number of pollen grains per anther, as well as the number of pollen grains produced per flower differed significantly between species (F4,32 = 15.851, P < 0.001; Table 1). The greatest number of pollen grains per anther and per flower was observed for O. flaemingina (2848.2 ± 656.0 and 22,785.6 ± 5247.8; mean ± SD; respectively), whereas the lowest values were seen in O. casimiri (981.6 ± 152.8 and 7852.8 ± 1222.7, respectively). Moreover, the number of ovules per flower was also found to vary between species (F4,32 = 69,224, P < 0.001), the greatest number of ovules per flower being recorded for O. paradoxa (i.e., 600.6 ± 25.5) and the lowest for O. casimiri (313.1 ± 22.4). The P/O ratio differed significantly between species (F4,32 = 11.868, P < 0.001), ranging from 23.4 ± 6.0 (O. rubricaulis) to 57.6 ± 16.4 (O. flaemingina). Furthermore, the viability of pollen grains ranged from 60.5 ± 5.2 to 77.1 ± 7.2% and was found also to differ between species (F4,113 = 29.737, P < 0.001; means calculated across 2 study years).

Field tests showed that peroxidase activity in the stigma commences prior to anthesis in all the species investigated. The stigmas were receptive at bud stage (approx. 1 day prior to anthesis) and remained receptive until the end of anthesis.

Breeding system

From the 800 flowers of all the species used experimentally, we collected 207, 211 and 169 capsules in 2013, 2014 and 2015, respectively. The remaining capsules had been partly consumed/destroyed by herbivores (for the analyses, we used only undamaged capsules) or could not be found during capsule harvesting. With the exception of those flowers where anthers had been removed and flowers bagged from bud stage and throughout anthesis (test for apomixis), capsules formed by flowers subjected to all pollination treatments contained seed. In general, all species differed in the number of seeds developed per capsule, subject to the different pollination treatments employed (Fig. 2; Table 2). Nevertheless, no common pattern was observed between species, and differences discovered were not always statistically significant. On the one hand, the lowest seed set was observed for emasculated and spontaneously cross-pollinated flowers (treatment IV) of O. flaemingina (151.8 ± 39.8; 37.4% of ovules), O. nuda (152.4 ± 37.5; 33.3% of ovules), O. paradoxa (252.5 ± 114.6; 42.0% of ovules), O. rubricaulis (137.6 ± 63.3; 39.3% of ovules) and in induced cross-pollinated flowers (treatment V) of O. casimiri (177.3 ± 83.6; 56.6% of ovules; data calculated over the 3 years period). On the other, the greatest number of seed sets occurred in open-pollinated control flowers (treatment I) of O. flaemingina (210.3 ± 26.7; 51.8% of ovules), O. nuda (205.1 ± 48.4; 45.2% of ovules), O. paradoxa (366.4 ± 92.9; 61.1% of ovules) and geitonogamously pollinated flowers (treatment II) of O. casimiri (264.6 ± 62.0; 84.5% of ovules) and O. rubricaulis (229.6 ± 39.3; 65.7% of ovules; data calculated over the 3 year period). In all species, intact control flowers produced as many seeds as hand-cross-pollinated flowers (Fig. 2). Considerable variation in average seed set in all Oenothera species was recorded between the 3 years of study for any given pollination treatment (Fig. 2), with more seed set observed for each species in 2015. Furthermore, the pollination treatment × year interaction was also significant for all species (Table 2).

Fig. 2
figure2

Average seed set ± SD (standard deviation) in five Oenothera species subjected to five pollination treatments in 2013–2015: (I) open pollination (control); (II) buds emasculated before the anthers dehisced, then hand-pollinated with pollen collected from the same individual (geitonogamy); (III) spontaneous self-pollination; (IV) spontaneous cross-pollination; and (V) induced cross-pollination. The numbers in brackets by the pollination treatment symbol indicate the number of harvested capsules. Means with the same lower case letter do not differ significantly between study years for a particular pollination treatment, whereas means with the same capital letter do not differ significantly between pollination treatments employed, at P < 0.05, based on the HSH Tukey test

Table 2 Results of two-way model analysis of variance (ANOVA) on number of seeds per capsule in five Oenothera species

Capsule length was significantly correlated with seed set for O. casimiri (Spearman rs = 0.69), O. flaemingina (rs = 0.66), O. nuda (rs = 0.78), O. paradoxa (rs = 0.71) and O. rubricaulis (rs = 0.82; P < 0.001). The longest capsules were produced in control open-pollinated flowers (treatment I) of O. casimiri (23.4 ± 4.1 mm), O. flaemingina (25.9 ± 1.9 mm), O. paradoxa (32.2 ± 2.2 mm) and in cases of geitonogamous pollination (treatment II) in O. nuda (26.1 ± 3.4 mm) and O. rubricaulis (23.6 ± 1.5 mm). The shortest capsules, on the other hand, occurred in spontaneously cross-pollinated flowers (treatment IV) of O. paradoxa (25.7 ± 4.3 mm), O. rubricaulis (20.2 ± 1.6 mm), O. nuda (22.3 ± 1.3 mm) and in induced cross-pollinated flowers (treatment V) of O. casimiri (20.4 ± 1.6 mm) and O. flaemingina (22.7 ± 0.9 mm; P < 0.05; means calculated across 3 study years).

Floral insect visitors

During each of the study seasons, both nocturnal and diurnal insects were seen visiting flowers of Oenothera. They belonged to four taxonomic orders, namely Coleoptera (Scarabaeidae: Oxythyrea funesta Poda); Diptera (Syrphidae: Episyrphus sp. Matsumura and Adachi); Hymenoptera (Apidae: Apis mellifera L., Bombus hortorum L., B. lapidarius L., B. pascuorum Scopoli, B. pratorum L., B. terrestris L.); and Lepidoptera (Noctuidae: Autographa gamma L., Pyrrhia umbra Hufnagel). Insects from orders Hymenoptera and Lepidoptera were observed in both 2013 and 2014 visiting all Oenothera species (Fig. 3). Conversely, species of fly (Diptera) were observed visiting only O. casimiri, O. nuda and O. pardoxa, whereas species of beetle (Coleoptera) visited only O. casimiri and O. flaemingina in 2013. Given that the last two insect taxa were observed only very rarely and represented no more than 4% of the total insect visits, we decided to exclude them from further analyses. Moreover, since flowers of Oenothera present pollinators with no morphological restrictions, we refer, hereafter, not to a particular insect species but to morphologically related (or functional sensu Fenster et al. 2004) groups of insects, i.e., Lepidoptera, honeybees (Apis mellifera) and bumblebees (Bombus spp.).

Fig. 3
figure3

Composition (%) of the floral insect visitors in five Oenothera species in a Lublin, SE Poland population, based on observations in 2013 and 2014

For all study seasons, most floral visits to Oenothera were made by nocturnal Lepidoptera. Indeed, the proportion of visits made by lepidopterans to any individual Oenothera species much exceeded 50% of all insect visits in both 2013 and 2014 (Fig. 3). Nocturnal Lepidoptera began visiting flowers of Oenothera prior to dusk, just after the first flowers opened, and the greatest activity of foraging was observed late in the evening (i.e., 21:00–23:00 GMT + 2 h). During visits to flowers, nocturnal Lepidoptera displayed specific foraging behavior; i.e., these insects landed exclusively at the edges of petals and consumed floral nectar from the hypanthium using their long proboscises. Remarkably, these insects were observed only extremely rarely to come into contact even with just one of the eight anthers or stigmatic lobes (Online Resource 1).

Bumblebees were the most frequently observed diurnal floral insect visitors to Oenothera, in both 2013 and 2014, representing 25.2% and 19.6 for O. casimiri, 13.9% and 24.0% for O. flaemingina, 28.1% and 35.9% for O. nuda, 27.0% and 12.5% for O. paradoxa and 24.7% and 19.1% for O. rubricaulis, respectively, for 2013 and 2014 (Fig. 3). Honeybees were not observed visiting O. nuda in any of the study years, whereas in other Oenothera species, honeybees were recorded relatively rarely, totalling some 5.2–10.5% of all insect visits (mean data for all plant species). Both bumblebees and honeybees began foraging early in the morning (i.e., approx. 5:00) and visited flowers until the onset of senescence (i.e., approx. 7:00–9:00), consuming both pollen and floral nectar.

Duration of insect visits and pollen loads analyses

Given that the species of Oenothera investigated in this paper do not vary significantly in the diversity or abundance of insects attracted to their flowers, we decided to pool the pollen load data and duration of insect visit data for the entire study period and for all plant species.

On average, the duration of insect visits to Oenothera flowers was 6.9 ± 4.1 s. However, the duration of visits differed considerably between groups of insects (Kruskal–Wallis test: H (2, N = 149) = 19.4348, P < 0.001). The longest visit to Oenothera was recorded for honeybees (i.e., 7.9 ± 3.2 s), whereas the shortest visit was observed for bumblebees (i.e., 5.1 ± 1.5 s).

In general, all captured insect visitors carried pollen of Oenothera and/or ‘other’ plant species. However, there were considerable differences in the number of pollen grains carried by the various insect groups, of both Oenothera pollen (Kruskal–Wallis test: H (2, N = 86) = 57.820, P < 0.001) and ‘other’ pollen (Kruskal–Wallis test: H (2, N = 86) = 25.383, P < 0.001). Although nocturnal Lepidoptera was the group of insects most frequently observed visiting flowers of Oenothera (Fig. 3), pollen load analyses revealed that these insects carried only very small numbers of pollen grains, of both Oenothera and ‘other’ species, on their bodies (i.e., 4.46 ± 4.32 and 22.50 ± 11.47, on average, respectively; Table 3). The greatest number of pollen grains of Oenothera was carried by honeybees (on average, 376.40 ± 355.31) and bumblebees (on average, 313.62 ± 211.28), and there was no significant difference between these groups of insect for Oenothera pollen loads (Kruskal–Wallis test: H (1, N = 56) = 0.198, P = 0.888). The greatest Oenothera pollen load for A. mellifera was 1740 pollen grains, whereas that for Bombus spp. was 685 pollen grains.

Table 3 Average number of pollen grains of Oenothera and ‘other’ plant species carried by the most frequent group of floral insect visitors (Diptera and Coleoptera excluded)

Discussion

Our results clearly indicate that all investigated Oenothera are self-compatible and reproduce sexually. Moreover, flowers emasculated and bagged throughout anthesis did not produce any seed, and thus, apomixis can be dismissed. Data from field investigations also revealed that the receptivity of the stigma and the release of pollen grains from anthers began at bud stage (approx. 24 h before anthesis), and the stigmas remained receptive until the onset of flower senescence. As a result of these developmental and temporal overlaps of male and female functions within flowers of Oenothera, self-pollination at the final bud stage (just before the flowers opened) was frequently observed. This phenomenon of self-pollination has been termed ‘prior selfing’ (or ‘bud autogamy’ sensu Noormets and Olson 2006, or ‘preanthesis cleistogamy’ sensu Culley and Klooster 2007) and has only rarely been recorded in self-fertilizing species (but see, e.g., Davis and Delph 2005; Bush 2009; Ling et al. 2017). We also demonstrated that bagged, intact flowers produced as many seeds as flowers subjected to hand-pollination treatments. This finding strongly indicates that the investigated species of Oenothera have a great capacity for autonomous selfing, which is consistent with the findings for other breeding system experiments on several members of subsect. Oenothera (Stubbe and Raven 1979; Kawano et al. 1995). Nevertheless, the pattern observed here does not apply to the whole genus, since both self-compatible and self-incompatible populations as well as variable populations have been reported for other sections and subsections of Oenothera (e.g., Gregory 1964; Wagner and Lammers 2005; Theiss et al. 2010).

In general, selection in favor of autonomous selfing is influenced by the degree of pollination variability, and selfing is particularly advantageous when the frequency of pollinator visits and/the reception of outcross pollen are low, as it offers reproductive assurance (Darwin 1876; Baker 1955; Goodwillie et al. 2005). According to Cruden (1977), analysis of the P/O ratio is a valuable indicator of breeding systems in flowering plants, and therefore, outcrossing rates within a species. This last author also suggested that in primarily self-pollinating taxa, investment in male fitness is expected to be lower compared to female fitness, and thus, the more efficient the transfer of pollen, the lower the P/O ratio. We observed that the P/O ratio of Oenothera was relatively low (33.9 ± 13.3, on average) and varied between species. Even so, the modified P/O ratio generally agreed well with that of other Oenothera spp., including the well-studied and closely related O. biennis and O. flava (Cruden and Jensen 1979; Summers et al. 2015). In Onagraceae, the reproductive structures are conspicuous because pollen grains are held by viscin threads and the stigmas are relatively large. Consequently, these characteristics probably contribute to the relatively low P/O ratio (Cruden and Jensen 1979). We thus propose that in the case of the Oenothera species investigated, a low P/O ratio is more closely associated with efficient pollen transfer onto the body of the pollinator than with shifts in outcrossing rates. Therefore, despite relatively low P/O ratios, it is proposed that large clumps of pollen coupled with the large surface area of the stigma contribute to successful pollination in these species. As such, it is also worth noting that significant variations in P/O ratio have also been demonstrated for several populations of O. flava in North America, with respect to geographical or temporal variability in pollinator abundance and mating opportunity (Summers et al. 2015).

Our study shows that flowers of the five Oenothera species, typically classified as ‘obligately autogamous’ (sensu Cruden 1977), may be fertilized either by autonomous (e.g., via prior selfing) or outcross pollen (after flower opening), indicating the potential for a mixed mating system (sensu Goodwillie et al. 2005). As a result, these plants, to different degrees, are subject to the dual advantages of both selfing and outcrossing. For example, autonomous selfing can precipitate pollination even when marked spatiotemporal variation in pollinator service is encountered, or mates are scarce. Indeed, we were able to demonstrate that emasculated flowers exposed to pollinators set fewer seeds than intact control flowers subject to open pollination; this difference, however, was not statistically significant for O. casimiri. This finding strongly indicates that for the species of Oenothera investigated here, selfing contributes appreciably to reproductive assurance (sensu Lloyd 1992). In addition, given that pollen availability and stigma receptivity were observed until the onset of flower senescence, it is proposed that competing and/or delayed selfing (either autonomous or vector mediated) may also occur during more advanced stages of floral development. A similar phenomenon is also thought to occur in the flowers of Tillandsia multicaulis Steud. (Bromeliaceae; Bush 2009) and Prunella vulgaris L. (Lamiaceae; Ling et al. 2017), in which self-fertilization was observed prior to flower opening, but seed production increased with progressing anthesis. In reality, however, the timing of autonomous self-pollination does not always fit into the three strict categories outlined here as it is continuously distributed in time, and therefore, selfing is always associated with some costs to both pollen and seed discounting (Lloyd 1992).

The breeding experiments were performed for only 3 years; however, we noted great variations in the total seed set between study years for most pollination treatments and all plant species. According to Nayor (2003), the number of seed produced is a phenotypic character that reflects the interaction between the genetic potential to produce seed and the environment experienced by the individual plant. This last author also suggested that environmental conditions during flowering and seed development may affect the proportion of fertilized ovules which are retained and the amount of reserves that they accumulate. Given that the anthesis of a single flower of the investigated species of Oenothera is relatively short (ca. 10-12 h), it is reasonable to suppose that ever-changing weather conditions (e.g., temperature, air humidity) may considerably impact on pollination and fertilization in these taxa. Indeed, it has been demonstrated that immediate and small-scale weather effects may act as constraints on the survival and germination of pollen grains, the exposure and receptivity of the stigmas, as well as pollinator activity in the field (e.g., Corbet 1990).

Although a substantial amount of autogamy appears to occur in the investigated Oenothera, a certain level of outcrossing is also needed in order to produce higher quality offspring and to create new PTH forms (Golczyk et al. 2014). We observed that flowers of Oenothera were visited by a great variety of potential pollinators, including both diurnal and nocturnal taxa. Remarkably, hawkmoths were not observed during our investigations for any study years, even though our experiments were conducted in a botanical garden likely to exhibit high insect biodiversity. It is generally agreed that the great requirement of pollinators for energy necessitates the uptake of much nectar (Nicolson 2007 and references therein). In the case of the species of Oenothera studied, insect visitors were attracted to the flower by both copious amounts of floral nectar (i.e., up to 26.8 mg/flower; Antoń et al. 2017) and pollen (i.e., up to 6.4 mg/flower; Antoń and Denisow 2018) that were constantly accessible from the onset of anthesis (i.e., evening hours) until the start of flower senescence (i.e., early morning hours). It is also worth noting that it has been proposed for other species of subsect. Oenothera that both visual (strong UV-absorbent spots near the center of the corolla) and chemical (volatiles with linalool as a primary constituent) signals participate in the attraction of potential pollinators to flowers (Kawano et al. 1995); however, these signals were not examined in our investigations. Even though, for every year of study, nocturnal lepidopterans were the most frequently encountered floral insect visitors (much exceeding 50% of all insect visits; see Fig. 3), our observations clearly show that these insects did not in any way contribute to the pollination of Oenothera. This was probably due to the specific behavior of these nocturnal lepidopterans during foraging, which differs significantly from that observed for hawkmoths. Indeed, it was demonstrated that during relatively long floral visits, these insects eagerly consumed floral nectar. Even so, they did not come into contact either with the anthers or with the lobes of the stigma (see Suppl. mat. 1). The findings were further confirmed during pollen load analyses, in that these insects carried extremely low numbers of Oenothera pollen grains on their bodies. Conversely, however, hawkmoth visits comprise a series of successive phases, including repeated backward and downward movements, hovering near the flower, the proboscis frequently entering the spur or nectar tube and thereby increasing the frequency of pollination events (Brantjes and Bos 1980). Consequently, given that during visits nocturnal lepidopterans did not come into direct contact with the reproductive parts of the flower, they can be considered ‘nectar thieves’ (sensu Inouye 1980), which, owing to the absence of visible damage to floral tissues indicative of typical nectar theft, here, seemingly have a negligible effect on plant reproduction. However, the direct effect of nectar theft by these insects on plant fitness was not examined in the present paper, and this phenomenon still requires further experimental testing.

Our observations showed that the true pollinators of Oenothera are probably members of family Apidae (i.e., honeybees and bumblebees), even though these insects contributed to significantly fewer visits and never exceeded more than 12% and 33% of total insect visits, respectively. Fidelity of these insects to Oenothera flowers is obvious from pollen load analyses. Indeed, in this study we demonstrated that generally, most honeybee and bumblebee pollen loads consisted mainly of Oenothera pollen grains (78.7% and 80.6%, respectively; Table 3). According to Dietrich et al. (1997), in chiefly self-pollinating Oenothera, floral rewards and visits by insects are less important. More recently, however, Ragusso et al. (2007) have shown that the high volumes of nectar present in small-flowered O. triloba (formerly referred to as autogamous) may indicate the potential for a mixed mating system, rather than strict autogamy. Our findings are consistent with those of the latter authors and demonstrate that, apart from prior selfing, visits by bees may lead to effective transfer of outcross pollen in the investigated species of Oenothera. Furthermore, in another, complementary project (Antoń et al. 2017), we reported that the complex pattern of nectar production and nectar distribution along the inflorescences of these taxa may determine the movement of pollinators between individual plants, thereby promoting cross-pollination. These observations all indicate that the breeding system present in subsect. Oenothera is much more intricate than previously thought, and therefore, these species should not be simply considered autogamous. Additionally, and in contrast to previous studies, which describe Oenothera plants as having generalized pollination systems (e.g., Waser et al. 1996), Krakos and Fabricant (2014) demonstrated that pollination systems in several Oenothera appear to be more pollinator specialized. The latter results, however, are limited only to outcrossing species from section Gaura, occurring in Northeast, Midwest, and Southwest of the USA.

It is also interesting to speculate on certain aspects of Oenothera pollination and breeding system strategies and their involvement in the dynamic spread of these taxa and their colonization of new localities. According to Tokhtar and Groshenko (2014), the representatives of subsect. Oenothera have become successfully established in Europe, and some taxa are considered to be actively invasive species, especially in Central and Eastern Europe. Invasion by alien plant species is recognized to be a serious threat to global biodiversity. However, despite growing concern over the negative aspects of plant invasion on various levels, surprisingly little is known about those attributes that make these plants such successful invaders (Richardson et al. 2000). The capacity of members of subsect. Oenothera to invade has been explained in terms of the similar weather conditions that prevail in Central Europe and which well match the climate present in their primary distribution range (Mihulka and Pyšek 2001), and/or their requirement for light in the initiation of seed germination (Mihulka et al. 2003). Here, we show that the potential of five species of subsect. Oenothera to invade is probably improved by a high degree of selfing (occurring inter alia at bud stage), followed by anthesis and opportunities for outcrossing, as well as the hybridization of new PTH forms. These characteristics are frequently associated with the invasive spread of species, especially within anthropogenic habitats (Denisow et al. 2017). We conclude that the species of Oenothera investigated in this paper are able to ensure autonomous reproductive advantage, especially when pollinators are scarce. These strategies probably enable a rapid increase in populations, as well as the vigorous establishment of colonies in new environments outside their primary distribution range, sites that are devoid of co-adapted fauna and flora.

References

  1. Antoń S (2015) Blooming biology, nectar production and role of the floral insect visitors in several Oenothera L. species (Onagraceae). In: XIII Konferencja Naukowa Doktorantów: “Problemy technologii produkcji roślinnej zwierzęcej i żywności”, Lublin, 23–24 marca 2015

  2. Antoń S, Denisow B (2018) Floral phenology and pollen production in the five nocturnal Oenothera species (Onagraceae). Acta Agrobot 71:1738. https://doi.org/10.5586/asbp.1738

  3. Antoń S, Komoń-Janczara E, Denisow B (2017) Floral nectary, nectar production dynamics and chemical composition in five nocturnal Oenothera species (Onagraceae) in relation to floral visitors. Planta 246:1051–1067

  4. Baker HG (1955) Self-compatibility and establishment after ‘long-distance’ dispersal. Evolution 9:347–348

  5. Bosisio M (1990) Squeezing oil from the primrose. Agric Res 38:20–21

  6. Brantjes NBM, Bos JJ (1980) Hawkmoth behaviour and flower adaptation reducing self pollination in two Liliflorae. New Phytol 84:139–143

  7. Bush JW (2009) Prior autonomous selfing in the hummingbird-pollinated epiphyte Tillandsia multicaulis (Bromeliaceae). Selbyana 30:114–121

  8. Charlesworth D, Charlesworth B (1987) Inbreeding depression and its evolutionary consequences. Annual Rev Ecol Evol Syst 18:237–268

  9. Charlesworth D, Morgan MT, Charlesworth B (1990) Inbreeding depression, genetic load, and the evolution of outcrossing rates a multilocus system with no linkage. Evolution 44:1469–1489

  10. Corbet SA (1990) Pollination and the weather. Israel J Bot 39:13–30

  11. Cruden RW (1977) Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution 31:32–46

  12. Cruden RW, Jensen KG (1979) Viscin threads, pollination efficiency and low pollen-ovule ratios. Amer J Bot 66:875–879

  13. Culley TM, Klooster MR (2007) The cleistogamous breeding system: a review of its frequency, evolution and ecology in angiosperms. Bot Rev 73:1–30

  14. Dafni A, Maues J (1998) A rapid and simple procedure to determine stigma receptivity. Sexual Pl Reprod 11:177–180

  15. Darwin C (1876) Effects of cross and self fertilization in the vegetable kingdom. John Murray, London

  16. Davis SL, Delph LF (2005) Prior selfing and gynomonoecy in Silene noctiflora L. (Caryophyllaceae): opportunities for enhanced outcrossing and reproductive assurance. Int J Pl Sci 166:475–480

  17. Denisow B, Wrzesień M, Mamchur Z, Chuba M (2017) Invasive flora within urban railway areas: a case study from Lublin (Poland) and Lviv (Ukraine). Acta Agrobot 70:1727. https://doi.org/10.5586/aa.1727

  18. Dietrich W, Wagner WL, Raven PH (1997) Systematics of Oenothera Sec. Oenothera subsection Oenothera (Onagraceae). Syst Bot Monogr 50:1–234

  19. Eckert CG (2002) Effect of geographical variation in pollinator fauna on the mating system of Decodon verticillatus (Lyrthaceae). Int J Pl Sci 163:123–132

  20. Fenster CB, Ambruster WS, Wilson P, Dudash MR, Thomson JD (2004) Pollination syndromes and floral specialization. Annual Rev Ecol Evol Syst 35:375–403

  21. Fornoni J, Nunez-Farfan J, Valverde PL, Rausher MD (2004) Evolution of mixed strategies of plant defense allocation against natural enemies. Evolution 58:1685–1695

  22. Golczyk H, Massouh A, Greiner S (2014) Translocation of chromosome end-segments and facultative heterochromatin promote meiotic ring formation in evening primroses. Pl Cell 26:1280–1293

  23. Goodwillie C, Kalisz S, Eckert CG (2005) The evolution of enigma of mixed mating systems in plants: occurrence, theoretical explanations, and empirical evidence. Annual Rev Ecol Evol Syst 36:47–79

  24. Gregory DP (1964) Hawkmoth pollination in the genus Oenothera. Aliso 5:385–419

  25. Inouye DW (1980) The terminology of floral larceny. Ecology 61:1251–1253

  26. Johnson MTJ (2011) The contribution of evening primrose (Oenothera biennis) to a modern synthesis of evolutionary ecology. Popul Ecol 53:9–21

  27. Kalisz S, Vogler DW (2003) Benefits of autonomous selfing under unpredictable pollinator environments. Ecology 84:2928–2942

  28. Kalisz S, Vogler DW, Hanley K (2004) Context-dependent autonomous self-fertilization yields reproductive assurance and mixed mating. Nature 430:884–887

  29. Kawano S, Odaki M, Yamaoka R, Oda-Tanabe M, Takeuchi M, Kawano N (1995) Pollination biology of Oenothera (Onagraceae). The interplay between floral UV-absorbancy patterns and floral volatiles as signals to nocturnal insects. Pl Spec Biol 10:31–38

  30. Krakos KN, Fabricant SA (2014) Generalized versus specialized pollination systems in Oenothera (Onagraceae). J Pollinat Ecol 14:235–243

  31. Krakos KN, Reece JS, Raven PH (2014) Molecular phylogenetics and reproductive biology of Oenothera section Kneiffia (Onagraceae). Syst Bot 39:523–532

  32. Lande R, Schemske DW (1985) The evolution of self-fertilization and inbreeding depression in plants. I. Genetic models. Evolution 39:24–40

  33. Ling TC, Wang LL, Zhang ZQ, Dafni A, Duan YW, Yang YP (2017) High autonomous selfing capacity and low flower visitation rates in a subalpine population of Prunella vulgaris (Lamiaceae). Pl Ecol Evol 150:59–66

  34. Lloyd DG (1980) Demographic factors and mating patterns in angiosperms. In: Solbrig OT (ed) Demography and evolution in plants populations. Blackwell, Oxford, pp 67–88

  35. Lloyd DG (1992) Self- and cross-fertilization in plants. II. The selection of self-fertilization. Int J Pl Sci 153:370–380

  36. Masuda M, Yahara T, Maki M (2004) Evolution of floral dimorphism in a cleistogamous annual, Impatiens noli-tangere L. occurring under different environmental conditions. Ecol Res 19:571–580

  37. Mihulka S, Pyšek P (2001) Invasion history of Oenothera congeners in Europe: a comparative study of spreading rates in the last 200 years. J Biogeogr 28:597–609

  38. Mihulka S, Pyšek P, Martínková J (2003) Invasiveness of Oenothera congeners in Europe related to their seed characteristics. In: Child LE, Brock JH, Brundu G, Prach K, Pyšek P, Wade M, Williamson M (eds) Plant invasions: ecological threats and management solutions. Backhuys Publishers, Leiden, pp 213–225

  39. Munoz F, Violle C, Cheptou PO (2015) CSR ecological strategies and plant mating systems: outcrossing increases with competiveness but stress-tolerance in related to mixed mating. Oikos 125:1296–1303

  40. Nayor REL (2003) Weed seed biology. In: Thomas B (eds.) Encyclopedia of Applied Plant Sciences, Elsevier, pp 1500-1508

  41. Nicolson SW (2007) Nectar consumers. In: Nicolson SW, Nepi M, Pacini E (eds) Nectaries and nectar. Springer, Dordrecht, pp 289–326

  42. Noormets M, Olson AR (2006) Bud-autogamy in the velvet-leaf blueberry, Vaccinium myrtilloides Michx. Canad J Pl Sci 86:245–250

  43. Raguso RA, Kelber A, Pfaff M, Levin RA, McDade LA (2007) Floral biology of North American Oenothera sect. Lavauxia (Onagraceae): advertisements, rewards, and extreme variation in floral depth. Ann Missouri Bot Gard 94:236–257

  44. Raven PH (1979) A survey of reproductive biology in Onagraceae. New Zealand J Bot 17:575–593

  45. Raven PH (1988) Onagraceae as a model of plant evolution. In: Gottlieb LD, Jain SK (eds) Plant evolutionary biology. Chapman & Hall, New York, pp 85–107

  46. Richardson DM, Pyšek P, Rejmánek M, Barbour MG, Panetta FD, West CJ (2000) Naturalization and invasion of alien plants: concepts and definitions. Diversity &  Distrib 6:93–107

  47. Rostański K (1985) Zur gliederung der subsection Oenothera (section Oenothera, Oenothera L., Onagraceae). Feddes Repert 96:3–14

  48. Rostański K, Tokarska-Guzik B (1998) Distribution of the American epecophytes of Oenothera L. in Poland. Phytocoenosis 10 Suppl. Cartogr Geobot 9:117–130

  49. Rostański K, Verloove F (2015) The genus Oenothera in Belgium. Dumortiera 106:12–42

  50. Rostański K, Dzhus M, Gudžinskas Z, Rostański A, Shevera M, Šulcs V, Tokhtar V (2004) The genus Oenothera L. in Eastern Europe. W. Szafer Institute of Botany, Kraków

  51. Rostański K, Rostański A, Gerold-Śmietańska I, Wąsowicz P (2010) Evening-Primroses (Oenothera) occuring in Europe, Wiesiołki (Oenothera) występujące w Europie. W. Szafer Institute of Botany, Katowice-Kraków

  52. Schoen DJ, Brown AHD (1991) Whole and part-flower self-pollination in Glycine clandestine and G. argyrea and the evolution of autogamy. Evolution 45:1651–1664

  53. Stone JL (2002) Molecular mechanism underlying the breakdown of gametophytic self-incompatibility. Quart Rev Biol 77:17–32

  54. Stubbe W, Raven PH (1979) Genetic Self-incompatibility in Oenothera subsect Euoenothera. Science 204:327

  55. Summers HE, Hartwick SM, Raguso RA (2015) Geographic variation in floral allometry suggests repeated transitions between selfing and outcrossing in a mixed mating plant. Amer J Bot 102:745–757

  56. Szklanowska K, Czubacki W (2000) The abundance and features of pollen in Oenothera L. genus representatives. Pszczel Zesz Nauk 2:225–265

  57. Theiss KE, Holsinger KE, Evans MEK (2010) Breeding system variation in 10 evening primroses (Oenothera sections Anogra and Kleinia; Onagraceae). Amer J Bot 97:1031–1039

  58. Tokhtar V, Groshenko S (2014) Differentiation of the climatic niches of the invasive Oenothera L. (subsect. Oenothera, Onagraceae) species in the Eastern Europe. Advances Environm Biol 8:529–531

  59. Vogler DW, Kalisz S (2001) Sex among the flowers: the distribution of plant mating systems. Evolution 55:202–204

  60. Wagner WL, Lammers TG (2005) Systematics of Oenothera sections Contortae, Eremia, and Ravenia (Onagraceae). Syst Bot 30:332–355

  61. Wagner WL, Hoch PC, Raven PH (2007) Revised classification of the Onagraceae. Syst Bot Monogr 83:1–240

  62. Waser NM, Chittka L, Price MV, Williams NM, Ollerton J (1996) Generalization in pollination systems, and why it matters. Ecology 77:1043–1060

  63. Wolfe LM, Barrett SCH (1988) Temporal changes in the pollinator fauna of tristylous Pontederia cordata, an aquatic plant. Canad J Zool 66:1421–1424

  64. Zych M (2007) On flower visitors and true pollinators: the case of protandrous Heracleum sphondylium L. (Apiaceae). Pl Syst Evol 263:159–179

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Acknowledgements

We thank Edyta Górska-Drabik, PhD, for help with the identification of floral insect visitors and Małgorzata Wrzesień, PhD, for help with plant species identification. All experimental and observation studies on protected insects were done in compliance with Polish law upon permissions DOP-oz.6401.01.61.2013.JRO from the General Directorate of Environment Protection and WPN.6401.96.2014.MPR from the Regional Conservator of Nature in Lublin. This research was supported financially by the Ministry of Science and Higher Education of Poland, as part of the statutory activities of the Department of Botany, University of Life Sciences in Lublin (Project OKB/MN/4).

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Antoń, S., Denisow, B. Pollination biology and breeding system in five nocturnal species of Oenothera (Onagraceae): reproductive assurance and opportunities for outcrossing. Plant Syst Evol 304, 1231–1243 (2018) doi:10.1007/s00606-018-1543-y

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Keywords

  • Bumblebees
  • Plant mating systems
  • Pollination
  • Prior selfing
  • Self-fertilization
  • Stigma receptivity