Nuclear microsatellite markers reveal the low genetic structure of Pinus mugo Turra (dwarf mountain pine) populations in Europe
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Twenty-one populations (555 individuals) covering the entire native range of Pinus mugo Turra (dwarf mountain pine) were investigated for genetic variation scored at 13 nuclear microsatellite markers (nSSRs). The main objective of the present study was to determine the genetic structure across the present distribution of the species and locate populations of different genetic compositions. Most of the genetic variation was observed within the populations (95%). The assignment of populations based on Bayesian clustering methods revealed that the Sudeten populations of P. mugo form a separate genetic cluster. These stands have likely been established through the founder effects of Alpine migrants. The distribution and level of SSR polymorphisms, along with no evidence of isolation by distance or phylogeographic structure, indicate that the present populations of P. mugo have diverged relatively recently and originate from a larger glacial distribution of the species. One peripheral stand from Italy had the lowest values of most calculated genetic variation indices. This stand could therefore be more susceptible to genetic drift and a negative impact of predicted environmental changes. We discuss our findings with respect to previously published results on the genetic and morphological variation of P. mugo and with consideration for the conservation genetics of the species.
KeywordsAdaptation Conservation genetics Genetic diversity Phylogeography Population differentiation Postglacial recolonization
The authors would like to thank K. Boratyńska and A. Boratyński for the collection of the plant material and M. Litkowiec for technical support and valuable comments concerning the data analysis. The work was financially supported through a grant from the Polish National Science Centre (Grant No. DEC-2012/05/E/NZ9/03476).
This study was funded through a grant from the Polish National Science Centre (Grant No. DEC-2012/05/E/NZ9/03476).
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Conflict of interest
The authors declare that they have no conflicts of interest.
- Boratyńska K, Muchewicz E, Drojma M (2004) Pinus mugo Turra geographic differentiation based on needle characters. Dendrobiology 51:9–17Google Scholar
- Boratyńska K, Dzialuk A, Lewandowski A, Marcysiak K, Jasińska AK, Sobierajska K, Tomaszewski D, Burczyk J, Boratyński A (2014) Geographic distribution of quantitative traits variation and genetic variability in natural populations of Pinus mugo in Central Europe. Dendrobiology 72:65–84. doi: 10.12657/denbio.072.006 CrossRefGoogle Scholar
- Chybicki I (2015) INEST 2.0. Available at: http://www.ukw.edu.pl/pracownicy/strona/igor_chybicki/software_ukw/
- Critchfield WB, Little EL (1971) Geographic distribution of the pines of the world. U.S. Department of Agriculture, WashingtonGoogle Scholar
- Dzialuk A, Boratyński A, Boratyńska K, Burczyk J (2012) Geographic patterns of genetic diversity of Pinus mugo (Pinaceae) in Central European mountains. Dendrobiology 68:31–41Google Scholar
- Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available at: http://www2.unil.ch/popgen/softwares/fstat.htm. Updated from Goudet (1995)
- Latałowa M, Tobolski K, Nalepka D (2004) Pinus L. subgenus Pinus (subgen. Diploxylon (Koehne) Pilger)—Pine. In: Ralska-Jasiewiczowa M (ed) Late glacial and Holocene history of vegetation in Poland based on isopollen maps. W. Szafer Institute of Botany, Kraków, pp 165–177Google Scholar
- Sobierajska K, Boratyńska K (2008) Variability of needle characteristics of Pinus mugo Turra populations in the Karkonosze Mountains in Poland. Dendrobiology 59:41–49Google Scholar
- Sobierajska K, Boratyńska K, Marcysiak K (2010) Variation of cone characters in Pinus mugo (Pinaceae) populations in the Giant Mountains (Karkonosze, Sudetes). Dendrobiology 63:33–41Google Scholar
- Willis KJ, Bennett KD, Birks HJB (1998) The late Quaternary dynamics of pines in Europe. In: Richardson DM (ed) Ecology and biogeography of Pinus. Cambridge University Press, Cambridge, pp 107–121Google Scholar