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Plant Systematics and Evolution

, Volume 302, Issue 10, pp 1381–1394 | Cite as

New species of Nostoc (cyanobacteria) isolated from Pune, India, using morphological, ecological and molecular attributes

  • Prashant Singh
  • Zaid M. Shaikh
  • Lira A. Gaysina
  • Archana Suradkar
  • Upasona Samanta
Original Article

Abstract

Filamentous cyanobacterium (strain NE-PS) isolated from a freshwater body in Pune, India, is being described as new species of the polyphyletic genus Nostoc. Phenotypic and molecular characterizations were performed, and the combined results validated the strain as a new species. Careful observations of the filaments, the presence of a distinct sheath throughout the length of the trichome, prominent differences in the shape and dimensions of the vegetative cells, heterocytes and the akinetes provided reliable morphological signals that the strain differed from rest of the closely related species. Sequencing of the 16S rRNA gene showed 98.66 % sequence similarity with Nostoc linckia, while rbcL and psbA sequencing showed 97 and 94 % similarities with Nostoc sp. PCC 7906 and Nostoc punctiforme PCC 73102, respectively, while the nifD gene sequence similarity was found to be 96 % with N. punctiforme Ind35 and Desmonostoc muscorum. The PC-IGS region was sequenced and concatenated cpcB, IGS and cpcA regions indicated the closest similarity with Nostoc linckia PACC 5085 at 96 %. Subsequent phylogenetic analyses gave a strong pattern of distinct clustering in case of all the molecular markers. The phenotypic, genetic and phylogenetic observations prove conclusively that the strain NE-PS is a new species in the genus Nostoc with the name proposed being Nostoc punensis, sp. nov.

Keywords

Cyanobacteria Evolution Phylogeny Systematics 

Notes

Acknowledgments

We thank the Director NCCS for facilities and encouragement. This work was supported by the Department of Biotechnology (DBT; Grant no. BT/PR/0054/NDB/52/94/2007), the Government of India, under the project ‘Establishment of Microbial Culture Collection’. L.A. Gaysina is thankful to the Russian Foundation for Basic Research in frame of projects 15-29-05893 ofr-i. We are immensely grateful to Mr. Yunir Gabidullin for helping us in preparation of all the plates and in arrangement of all the images.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

606_2016_1337_MOESM1_ESM.pdf (405 kb)
Online Resource 1 A general view of the trichomes of the strain NE-PS (40X). (PDF 405 kb)
606_2016_1337_MOESM2_ESM.pdf (449 kb)
Online Resource 2 Loosely arranged trichomes with a view of the vegetative cells, heterocytes (both terminal and intercalary) (40X). (PDF 448 kb)
606_2016_1337_MOESM3_ESM.pdf (466 kb)
Online Resource 3 Arrangement of trichomes with the constrictions between cells being visible noticeably (40X). (PDF 466 kb)
606_2016_1337_MOESM4_ESM.pdf (423 kb)
Online Resource 4 Trichome arrangement with terminal heterocytes having one and two polar nodules (40X). (PDF 423 kb)
606_2016_1337_MOESM5_ESM.pdf (1.4 mb)
Online Resource 5 Plate showing general view of routine trichomes with characteristic features of the strain NE-PS (40X) (PDF 1442 kb)
606_2016_1337_MOESM6_ESM.pdf (419 kb)
Online Resource 6 1—trichome with intercalary heterocytes (40X); 2—trichomes with terminal heterocytes (40X). (PDF 418 kb)
606_2016_1337_MOESM7_ESM.pdf (434 kb)
Online Resource 7 Neighbor Joining tree (NJ) tree of the strain NE-PS based on the 16S rRNA gene. (PDF 434 kb)
606_2016_1337_MOESM8_ESM.pdf (427 kb)
Online Resource 8 Maximum parsimony tree (MP) tree of the strain NE-PS based on the 16S rRNA gene. (PDF 426 kb)
606_2016_1337_MOESM9_ESM.pdf (425 kb)
Online Resource 9 Neighbor Joining tree (NJ) tree of the strain NE-PS based on the rbcL gene. (PDF 425 kb)
606_2016_1337_MOESM10_ESM.pdf (419 kb)
Online Resource 10 Maximum parsimony tree (MP) tree of the strain NE-PS based on the rbcL gene. (PDF 418 kb)
606_2016_1337_MOESM11_ESM.pdf (412 kb)
Online Resource 11 Neighbor Joining tree (NJ) tree of the strain NE-PS based on the psbA gene. (PDF 412 kb)
606_2016_1337_MOESM12_ESM.pdf (404 kb)
Online Resource 12 Maximum parsimony tree (MP) tree of the strain NE-PS based on the psbA gene. (PDF 404 kb)
606_2016_1337_MOESM13_ESM.pdf (416 kb)
Online Resource 13 Neighbor Joining tree (NJ) tree of the strain NE-PS based on the nifD gene. (PDF 416 kb)
606_2016_1337_MOESM14_ESM.pdf (411 kb)
Online Resource 14 Maximum parsimony tree (MP) tree of the strain NE-PS based on the nifD gene. (PDF 410 kb)
606_2016_1337_MOESM15_ESM.pdf (414 kb)
Online Resource 15 Neighbor Joining tree (NJ) tree of the strain NE-PS based on the PC-IGS region. (PDF 414 kb)
606_2016_1337_MOESM16_ESM.pdf (410 kb)
Online Resource 16 Maximum parsimony tree (MP) tree of the strain NE-PS based on the PC-IGS region. (PDF 409 kb)

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Prashant Singh
    • 1
  • Zaid M. Shaikh
    • 1
  • Lira A. Gaysina
    • 2
    • 3
  • Archana Suradkar
    • 1
  • Upasona Samanta
    • 1
  1. 1.Microbial Culture Collection (MCC)National Centre for Cell Science (NCCS)PuneIndia
  2. 2.Department of Bioecology and Biological EducationM. Akmullah Bashkir State Pedagogical UniversityUfaRussian Federation
  3. 3.All-Russian Research Institute of PhytopathologyMoscow RegionRussian Federation

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