Phylogenetic relationships of Thinopyrum and Triticum species revealed by SCoT and CDDP markers
- 416 Downloads
To evaluate the phylogenetic relationships among Thinopyrum species and Triticum species, 7 accessions of Thinopyrum species (2 Th. bessarabicum, 1 Th. elongatum, 2 Th. intermedium and 2. Th. ponticum), 11 accessions of Triticum species (2 Aeglips tauschii, 1 T. monococcum, 1 T. turgidum, 2 T. timopheevii and 5 T. aestivum) together with Hordeum vulgare cv. Golden were analysed using 17 SCoT and 10 CDDP markers. The mean number of observed alleles was 8.5 and 6.6 among the species for SCoT and CDDP markers, respectively. Based on the genetic data produced by the SCoT and CDDP markers, cluster analysis among Thinopyrum species, Triticum species and Hordeum was performed to generate dendrograms. The genetic relationships among Thinopyrum species, Triticum species and H. vulgare revealed by the SCoT markers were in agreement with the results of the CDDP markers. On both dendrograms, these species formed three clusters. The results indicated that Thinopyrum species and Triticum species were the most closely related, whereas H. vulgare was relatively distant from both genera. In addition, seven markers, i.e. SCoT 9, SCoT 31, SCoT 34, WRKY-R1, WRKY-R2, MADS-1 and MADS-4 were developed for monitoring introgression of Thinopyrum chromosomes or chromosome segments into Triticum species.
KeywordsMarker-assisted introgression Phylogenetic relationships SCoT and CDDP markers Thinopyrum species Triticum species
We thank Dr. Honggang Wang, Shandong Agricultural University (SDAU), Taian 271018, China, and Dr. Herbert Ohm, Purdue University, West Lafayette, IN 47907-1150, USA for providing seeds of Thinopyrum species. We also thank Dr. Lihui Li, Insititute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China, and Dr. H. Bockelman, NPGS, ARS, USA for providing seeds of Triticum species. We also acknowledge financial supports by the National High-Tech R&D Program of China (2011AA100102 and 2012AA101105), the NSF of China (Grant No. 31171553 and 31471488), Shandong Seed Engineering Project (2015–2019) and the International Collaboration Program (948 Project, 2013-S19).
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
- Guo J, Zhang X, Hou Y, Cai J, Shen X, Zhou T, Xu H, Ohm HW, Wang H, Li A, Han F, Wang H, Kong L (2015b) High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet 128:2301–2316. doi: 10.1007/s00122-015-2586-x CrossRefPubMedGoogle Scholar
- Hamidi H, Talebi R, Keshavarzi F (2014) Comparative efficiency of functional gene-based markers, start codon targeted polymorphism (SCoT) and conserved DNA-derived polymorphism (CDDP) with ISSR markers for diagnostic fingerprinting in wheat (Triticum aestivum L.). Cereal Res Commun 42:558–567. doi: 10.1556/CRC.2014.0010 CrossRefGoogle Scholar
- Huang S, Sirikhachornkit A, Su X, Faris J, Gill B, Haselkorn R, Gornicki P (2002) Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat. Proc Natl Acad Sci USA 99:8133–8138. doi: 10.1073/pnas.072223799 CrossRefPubMedPubMedCentralGoogle Scholar
- Oliver R, Xu S, Stack R, Friesen T, Jin Y, Cai X (2006) Molecular cytogenetic characterization of four partial wheat-Th. ponticum amphiploids and their reactions to Fusarium head blight, tan spot, and Stagonospora nodorum blotch. Theor Appl Genet 112:1473–1479. doi: 10.1007/s00122-006-0250-1 CrossRefPubMedGoogle Scholar
- Rohlf J (1997) NTSYS-pc 2.10e: Numerical taxonomy and multivariate analysis system. Applied Biostatistics Inc., SetauketGoogle Scholar
- Sneath PH, Sokal RR (1973) Numerical taxonomy. The principles and practice of numerical classification, W.H. Freeman and Company, San Francisco, USAGoogle Scholar
- Wang RRC, Von Bothmer R, Dvorak J, Fedak G, Linde-Laursen I, Muramatsu M (1994) Genome symbols in the Triticeae (Poaceae). In: Proceedings of the 2nd international Triticeae symposium. Logan, pp 20–24Google Scholar
- Xu S, Niu Z, Klindworth D, Zhang Q, Chao S, Friesen T, Jin Y, Rouse M, Faris J, Cai X (2013) Chromosome engineering for alien gene introgression in wheat: progress and prospective. Meeting Abstract, pp 24–25Google Scholar