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Plant Systematics and Evolution

, Volume 302, Issue 4, pp 357–366 | Cite as

Evidence of the production of 2n eggs in diploid plants of the autopolyploid complex Turnera sidoides L. (Passifloraceae)

  • Ivana E. Kovalsky
  • Viviana G. Solís Neffa
Original Article

Abstract

Turnera sidoides is a complex of dystilous perennial rhizomatous herbs with five subspecies in which diploid (2n = 2x = 14) to octoploid (2n = 8x = 56) cytotypes were found. Previous studies have suggested an autopolyploid origin of the complex, and provided evidence for the production of 2n pollen in experimental conditions as well as in natural populations. However, only the production of 2n pollen has been demonstrated so far, while the production of 2n eggs on experimental crosses or in natural populations of T. sidoides remains unknown. In this paper we investigate the production of 2n eggs in diploid individuals to understand how they may have contributed to the origin and establishment of polyploids in diploid populations of T. sidoides. Our progeny test and flow cytometric analysis of seeds collected in natural populations of this species complex, show that the triploid embryos originated from 2n eggs, suggesting that 2n eggs can contribute to the origin of neopolyploids by sexual unilateral polyploidization as well as by sexual bilateral polyploidization. The occurrence of plants that continuously form many 2n eggs and pollen would play a key role in the establishment of neopolyploids in natural diploid populations.

Keywords

2n eggs Polyploidy Sexual polyploidization Turnera sidoides 

Notes

Acknowledgments

We are deeply indebted to anonymous referees for their helpful comments on earlier version of this manuscript. This research was partially supported by Grants of the Agencia Nacional de Promoción Científica, Tecnológica y de Innovación (ANPCyT- FONCyT, PICT 07-1329 and PICT 12-1812), the National Research Council of Argentina (CONICET, PIP 11220120100192CO), and the Secretaría General de Ciencia y Técnica de la Universidad Nacional del Nordeste (SGCyT-UNNE, PI-A003/10) to V. G. Solís Neffa. I. E. Kovalsky is a Post Doctoral Fellow of CONICET and the Northeastern National University (UNNE). V. G. Solís Neffa is a member of the Carrera del Investigador Científico of CONICET.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Ivana E. Kovalsky
    • 1
    • 2
  • Viviana G. Solís Neffa
    • 1
    • 2
  1. 1.Laboratorio de Citogenética y Evolución VegetalInstituto de Botánica del Nordeste (UNNE-CONICET)CorrientesArgentina
  2. 2.Facultad de Ciencias Exactas y Naturales y Agrimensura (UNNE)CorrientesArgentina

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