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Genetic analysis of the dry forest timber tree Sideroxylon capiri in Costa Rica using AFLP

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Abstract

Sideroxylon capiri (“Tempisque”) is a threatened and economically important timber species in the dry forest of Costa Rica. To assess the extent of the genetic diversity between and within populations of this species, 86 samples were obtained from four sites in the northwestern part of the country from protected and non-protected areas. They were analyzed by amplified fragment length polymorphism. Five primers were used to generate 254 polymorphic bands. Molecular variance indicated a 92 % within locations with a PhiPT (φ st) of 0.083 and a He from 0.204 to 0.249. PCoA analysis showed two different groups: one formed by Palo Verde and La Cruz samples, and another group with Nicoya and Barra Honda samples. The dendrogram arranged the samples in three groups: individuals from Palo Verde and La Cruz divided in two groups, and a more compact group from Barra Honda and Nicoya. A positive correlation between pairwise linearized geographical distance and genetic differentiation among populations was detected by Mantel test (R 2 = 0.76241, P = 0.04). Results are discussed in terms of fragmentation of the continuous forests and geographical barriers. Despite these results, it is evident that a great genetic diversity exists for this species and the results can be used for conservation purposes.

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Acknowledgments

The authors thank the Vicerrectoría de Investigación de la Universidad de Costa Rica, Consejo Nacional de Ciencia y Tecnología de Costa Rica, Sistema Nacional de Áreas de Conservación (SINAC), Ministerio de Ambiente y Energía (MINAE), University of Queensland, University of Adelaide, Dr. Peter Prentis, Dr. Micheal Gardner, Dr. Giselle Tamayo, Dr. Rosaura Romero and Dr. James Karkashian.

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Authors and Affiliations

  1. Centro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, 11501-2060, San José, Costa Rica

    Heidy M. Villalobos-Barrantes & Federico J. Albertazzi

  2. Escuela de Química, Universidad de Costa Rica, 2060, San José, Costa Rica

    Heidy M. Villalobos-Barrantes

  3. Escuela de Biología, Universidad de Costa Rica, 2060, San José, Costa Rica

    Elmer G. García & Federico J. Albertazzi

  4. School of Earth and Environmental Sciences, University of Adelaide, North Terrance, SA, 5005, Australia

    Andrew J. Lowe

  5. Department of Environment Water and Natural Resources, Science Resource Centre, State Herbarium of South Australia, Hackney, SA, 5005, Australia

    Andrew J. Lowe

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  1. Heidy M. Villalobos-Barrantes
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  2. Elmer G. García
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  3. Andrew J. Lowe
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  4. Federico J. Albertazzi
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Correspondence to Heidy M. Villalobos-Barrantes.

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Villalobos-Barrantes, H.M., García, E.G., Lowe, A.J. et al. Genetic analysis of the dry forest timber tree Sideroxylon capiri in Costa Rica using AFLP. Plant Syst Evol 301, 15–23 (2015). https://doi.org/10.1007/s00606-014-1049-1

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