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Plant Systematics and Evolution

, Volume 301, Issue 1, pp 15–23 | Cite as

Genetic analysis of the dry forest timber tree Sideroxylon capiri in Costa Rica using AFLP

  • Heidy M. Villalobos-BarrantesEmail author
  • Elmer G. García
  • Andrew J. Lowe
  • Federico J. Albertazzi
Original Article

Abstract

Sideroxylon capiri (“Tempisque”) is a threatened and economically important timber species in the dry forest of Costa Rica. To assess the extent of the genetic diversity between and within populations of this species, 86 samples were obtained from four sites in the northwestern part of the country from protected and non-protected areas. They were analyzed by amplified fragment length polymorphism. Five primers were used to generate 254 polymorphic bands. Molecular variance indicated a 92 % within locations with a PhiPT (φ st) of 0.083 and a He from 0.204 to 0.249. PCoA analysis showed two different groups: one formed by Palo Verde and La Cruz samples, and another group with Nicoya and Barra Honda samples. The dendrogram arranged the samples in three groups: individuals from Palo Verde and La Cruz divided in two groups, and a more compact group from Barra Honda and Nicoya. A positive correlation between pairwise linearized geographical distance and genetic differentiation among populations was detected by Mantel test (R 2 = 0.76241, P = 0.04). Results are discussed in terms of fragmentation of the continuous forests and geographical barriers. Despite these results, it is evident that a great genetic diversity exists for this species and the results can be used for conservation purposes.

Keywords

AFLP Genetic diversity Tropical dry forest Tempisque Sapotaceae 

Notes

Acknowledgments

The authors thank the Vicerrectoría de Investigación de la Universidad de Costa Rica, Consejo Nacional de Ciencia y Tecnología de Costa Rica, Sistema Nacional de Áreas de Conservación (SINAC), Ministerio de Ambiente y Energía (MINAE), University of Queensland, University of Adelaide, Dr. Peter Prentis, Dr. Micheal Gardner, Dr. Giselle Tamayo, Dr. Rosaura Romero and Dr. James Karkashian.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Heidy M. Villalobos-Barrantes
    • 1
    • 2
    Email author
  • Elmer G. García
    • 3
  • Andrew J. Lowe
    • 4
    • 5
  • Federico J. Albertazzi
    • 1
    • 3
  1. 1.Centro de Investigación en Biología Celular y MolecularUniversidad de Costa RicaSan JoséCosta Rica
  2. 2.Escuela de QuímicaUniversidad de Costa RicaSan JoséCosta Rica
  3. 3.Escuela de BiologíaUniversidad de Costa RicaSan JoséCosta Rica
  4. 4.School of Earth and Environmental SciencesUniversity of AdelaideNorth TerranceAustralia
  5. 5.Department of Environment Water and Natural Resources, Science Resource CentreState Herbarium of South AustraliaHackneyAustralia

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