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Plant Systematics and Evolution

, Volume 300, Issue 8, pp 1771–1777 | Cite as

Characterization of B chromosomes in Lilium hybrids through GISH and FISH

  • Songlin Xie
  • Agnieszka Marasek-Ciolakowska
  • Munikote S. Ramanna
  • Paul Arens
  • Richard G. F. Visser
  • Jaap M. van Tuyl
Original Article

Abstract

Supernumerary (B) chromosomes and small aberrant chromosomes were detected in Lilium hybrids and characterized through genomic in situ hybridization (GISH) and florescence in situ hybridization (FISH). Two small, supernumerary or B chromosomes were detected as extra chromosomes in a tetraploid plant derived from chromosome doubling of a hybrid (2n = 2x = 24) between a cultivar of the Longiflorum (L) and the Trumpet (T) group. When this tetraploid LLTT hybrid was crossed with a triploid LLO hybrid (O = Oriental), the B chromosome was transmitted to 73.4 % of the progenies. Based on GISH and FISH characterization, it was shown that the B chromosome consisted of two identical arms, with 5S rDNA hybridizing to the majority of it, which were flanked by normal telomeres, suggesting that this is an isochromosome. In another population, which is a backcross progeny between a F1 hybrid of Longiflorum × Asiatic (LA) and its Asiatic parent, the former produced functional 2n gametes which resulted in a triploid LAA progeny (2n = 3x = 36), in which three exceptional plants possessed 35 normal chromosomes and a small aberrant chromosome instead of the expected normal number of 36. In all three cases, the small aberrant chromosomes were isochromosomes which had obviously originated during the first backcross generation. These three chromosomes showed normal telomeres and mitosis. In addition, one of the new generated chromosomes possessed two 45S rDNA sites in the proximal positions. These new arisen isochromosomes were proposed to originate from centric breakage and fusion of two short arms of the missing chromosome in three genotypes, respectively, based on the comparison of arm lengths as well as rDNA loci. Their relevance to the origin of Bs is discussed.

Keywords

Lily B chromosomes Isochromosome Centromere misdivision rDNA 

Abbreviations

GISH

Genomic in situ hybridization

FISH

Florescence in situ hybridization

Bs

B chromosomes

rDNA

Ribosomal DNA

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Songlin Xie
    • 1
    • 3
  • Agnieszka Marasek-Ciolakowska
    • 1
    • 2
  • Munikote S. Ramanna
    • 1
  • Paul Arens
    • 1
  • Richard G. F. Visser
    • 1
  • Jaap M. van Tuyl
    • 1
  1. 1.Wageningen UR Plant BreedingWageningen University and Research CentreWageningenThe Netherlands
  2. 2.Laboratory of Biochemistry and Molecular Biology, Department of General and Molecular BiologyResearch Institute of HorticultureSkierniewicePoland
  3. 3.Sino-Europe Agricultural Development Centre (SEADC)ZhangzhouPeople’s Republic of China

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