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Plant Systematics and Evolution

, Volume 298, Issue 6, pp 1195–1210 | Cite as

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

  • Tao Wan
  • Xiao-lin Zhang
  • Juraj Gregan
  • Ying Zhang
  • Pin Guo
  • You-hao Guo
Short Communication

Abstract

In this study, we analyzed 12 species in the subgenus Potamogeton (8 tetraploids and 2 diploids, 2 putative tetraploid hybrids) at the chromosomal level, including counting the chromosome number and physically mapping the rDNA. The extent of variation in the chromosome number and rDNA loci was determined in the Potamogeton species on both the inter- and intra-specific level. Moreover, one of the hybrid sets (P. perfoliatus ♂ × P. wrightii ♀) was picked for performing artificial pollination for producing F1 generation adults and for sequential morphological character analysis and FISH detection. After comparing the parental species and natural hybrids from three different geographic locations, the intraspecific variations of rDNA loci were revealed in sampled plants; in addition, comparisons between artificial F1 and natural hybrids (P. × intortusifolius) showed a rapid change of 45S rDNA loci in response to the interspecific hybridization. We also compared our results concerning rDNA patterns with phylogenetic documents to derive complementary clues to karoytype evolution and interspecific relationships. Based on frequent hybridizations and active clonal reproduction with weak genetic selection, the rDNA chromosomal repatterning, such as the gain or loss of rDNA loci together with rDNA movements, might be one trend of chromosome evolution in this genus.

Keywords

Potamogeton Interspecific hybridization Physical mapping of rDNAs Karyotype evolution Chromosome evolution 

Notes

Acknowledgments

The research was supported by the National Natural Science Foundation of China (no. 30430050). We are grateful to Prof. L.J. Li, of the Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, for kindly providing the rDNA probe and his kind advice on our work. We are also indebted to Prof. C.Y. Song, Wuhan University, and Prof. D. M. Zhang, the Laboratory of Systematic and Evolutionary Botany, Chinese Academy of Sciences, for helpful comments on the manuscript. We thank Prof. Q.F. Wang, Wuhan Arboretum of the Chinese Academy of Sciences, for providing the field for the preservation of experimental plants.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Tao Wan
    • 1
  • Xiao-lin Zhang
    • 2
  • Juraj Gregan
    • 3
  • Ying Zhang
    • 1
  • Pin Guo
    • 1
  • You-hao Guo
    • 1
  1. 1.Laboratory of Plant Systematics and Evolutionary BiologyCollege of Life Science, Wuhan UniversityWuhanChina
  2. 2.Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  3. 3.Max F. Perutz Laboratories, Department of Chromosome BiologyUniversity of ViennaViennaAustria

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