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Plant Systematics and Evolution

, Volume 298, Issue 1, pp 239–250 | Cite as

Reproductive characterization and preliminary studies on controlled breeding of Annatto (Bixa orellana L.)

  • Nisha Joseph
  • E. A. Siril
  • G. M. Nair
Original Article

Abstract

Annatto (Bixa orellana L.) is an important dye-yielding medium-sized tree occurring in the tropics. Investigations aimed at elucidating the reproductive characters as well as establishing a set of breeding parameters for genetic improvement of B. orellana was carried out. Records on pollen/ovule ratios of annatto showed that this species is xenogamous. The acetolyzed pollen of B. orellana was tricolporate. The fluorochromatic test showed 95% pollen viability. The viability of the pollen grains decreased sharply after 4 h from anther dehiscence. The optimum time range for artificial pollination was standardized between 12:00 and 13:00 h. A high in vitro germination rate (92%) of freshly collected pollen was recorded in modified Brewbaker’s medium with 15% sucrose. Resource allocation studies showed that the construction of each flower cost 1.162 g. The floral vegetative cost was 75%. Of the total allocation, 21% was spent by the stamens. Based on identified reproductive characters, controlled pollination experiments were conducted by selecting four morphologically distinct parent plants: (1) green-fruited with reddish spines, (2) red-fruited, (3) brown large-fruited, and (4) with brown distorted fruits. In artificial cross-pollination experiments, wide variation ranges in fruit set (0–95%) and fruit maturation (0–85%) were observed in different crosses. The number of seeds per capsule also showed variation (0–40 per capsule). Germination studies of seeds raised from different crosses showed a maximum 93% germination. The present study proved that improvement in B. orellana could be achieved by adopting breeding techniques using specifically selected parents.

Keywords

Bixa orellana Pollen viability In vitro pollen germination Resource allocation Controlled pollination 

Notes

Acknowledgments

The authors are grateful to Dr. Ashalatha S. Nair, Professor and Head, Department of Botany, University of Kerala, for providing facilities. The authors also thank Kerala State Council for Science Technology Environment (KSCSTE), Government of Kerala, Thiruvananthapuram, India, for financial support (no. 028, SRSLS/2007/CSTE).

References

  1. Avila A, Barquero L, Calzada J (1982) An improved method for detection of bixin from annatto seeds. Ing Cienc Quim 6:209–210Google Scholar
  2. Brewbaker JL, Kwack BH (1963) The essential role of calcium ion in pollen germination and pollen tube growth. Am J Bot 50:859–865CrossRefGoogle Scholar
  3. Casper BB, Niesenbaum RA (1993) Pollen versus resource limitation of seed production-A consideration. Curr Sci 31:32–46Google Scholar
  4. Chopra RN, Kaur H (1965) Embryology of Bixa orellana Linn. Phytomorphology 15:211–214Google Scholar
  5. Cour P, Van Campo M (1980) Prevision de recoletes a partir de I’analyse du contenue pollinique de l’atmosphere. C R Acad Sci (Paris) 290:1043–1046Google Scholar
  6. Cruden RW (1977) Pollen–ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution 31:32–46CrossRefGoogle Scholar
  7. Dafni A, Motte MM (1998) A rapid and simple procedure to determine stigma receptivity. Sex Plant Reprod 11:177–180CrossRefGoogle Scholar
  8. Dafni A, Kevan PG, Husband BC (2005) Practical pollination biology. Enviroquest, OntarioGoogle Scholar
  9. Erdtman G (1952) Pollen morphology and plant taxonomy: angiosperms. Almqvist and Wiksell, StockholmGoogle Scholar
  10. Gibbs PE, Milne C, Carrillo M (1975) Correlation between the breeding system and recombination index in five species of Senecio. New Phytol 75:619–626CrossRefGoogle Scholar
  11. Heslop-Harrison J, Heslop-Harrison Y (1987) Pollen germination and pollen tube growth. Int Rev Cytol 107:1–78CrossRefGoogle Scholar
  12. Janssen AW, Hermsen JG (1976) Estimating pollen fertility in Solanum species and haploids. Euphytica 25:577–586CrossRefGoogle Scholar
  13. Johri BM, Vasil IK (1961) Physiology of pollen. Bot Rev 27:325–381CrossRefGoogle Scholar
  14. Kearns CA, Inouye DW (1993) Techniques for pollination biologists. Colorado University Press, ColoradoGoogle Scholar
  15. Khatun S, Flowers TJ (1995) The estimation of pollen viability in rice. J Exp Bot 46:151–154CrossRefGoogle Scholar
  16. Martin FW (1959) Staining and observing pollen tubes by means of fluorescence. Stain Technol 34:125–128PubMedGoogle Scholar
  17. Mione T, Anderson GJ (1992) Pollen–ovule ratios and breeding system evolution in Solanum section Basarthrum (Solanaceae). Am J Bot 79:279–287CrossRefGoogle Scholar
  18. Nair PKK (1970) Pollen morphology of angiosperms. Vikas, New DelhiGoogle Scholar
  19. Preston RE (1986) Pollen–ovule ratios in the Cruciferae. Am J Bot 73:1732–1740CrossRefGoogle Scholar
  20. Rivera-Madrid R, Escobedo GMRM, Galera EB, Vera-Ku M, Harries H (2006) Preliminary studies towards genetic improvement of annatto (Bixa orellana L.). Sci Hort 109:165–172CrossRefGoogle Scholar
  21. Rodriguez G, Enriquez G (1983) Estudio preliminary del desarrollo de ramas y la biologia floral en Bixa orellana L. In: Arce J (ed) Aspectos sobre el achiote y perspectivas para Costa Ric, Turrialba. CATIE, Turrialba, Costa Rica, pp 58–76Google Scholar
  22. Rodriguez-Riano T, Dafni A (2000) A new procedure to assess pollen viability. Sex Plant Reprod 12:241–244CrossRefGoogle Scholar
  23. Ronse DLP (1989) Floral development of Cochlospermum tinctorium and Bixa orellana with special emphasis on the androecium. Am J Bot 76:1344–1359CrossRefGoogle Scholar
  24. Shivanna KR, Johri BM (1985) The angiosperm pollen: structure and function. Wiley Eastern, New DelhiGoogle Scholar
  25. Shivanna KR, Johri BM (1989) Angiosperm pollen: structure and function. Wiley, New YorkGoogle Scholar
  26. Shivanna KR, Rangaswami NS (1992) Pollen biology. A laboratory manual. Springer, BerlinCrossRefGoogle Scholar
  27. Smith J (2006) Annatto extracts. Chemical and technical assessment. Food and Agriculture Organization of the United Nations. ftp://ftp.fao.org/agn/jecfa/cta-annatto.pdf. Accessed 14 September 2011
  28. Snedecor GW, Cochran WG (1962) Statistical methods. Iowa State University Press, IA, p 318Google Scholar
  29. Spira TP, Snow AA, Whigham DF, Leak J (1992) Flower visitation, pollen deposition, and pollen-tube competition in Hibiscus moscheutos (Malvaceae). Am J Bot 79:428–433CrossRefGoogle Scholar
  30. Stanley RG, Linskens HF (1974) Pollen: biology biochemistry and management. Springer, BerlinGoogle Scholar
  31. Steer MW, Steer JM (1989) Pollen tube tip growth. New Phytol 111:323–358CrossRefGoogle Scholar
  32. Stone JL, Thomson JD, Dent-Acosta SJ (1995) Assessment of pollen viability in hand-pollination experiments: a review. Am J Bot 82:1186–1197CrossRefGoogle Scholar
  33. Tangmitchareon S, Owens JN (1997) Floral biology, pollination, pistil receptivity, and pollen tube growth of teak (Tectona grandis Linn. f.). Ann Bot 79:227–241CrossRefGoogle Scholar
  34. Tel-Zur N, Schneider B (2009) Floral biology of Ziziphus mauritiana (Rhamnaceae). Sex Plant Reprod 22:73–85PubMedCrossRefGoogle Scholar
  35. Tuinstra MR, Wedel J (2000) Estimation of pollen viability in grain sorghum. Crop Sci 40:968–970CrossRefGoogle Scholar
  36. Valdez-Ojeda R, Hernandez-Stefanoni JL, Aguilar-Espinosa M, Rivera-Madrid R, Ortiz R, Quiros CF (2008) Assessing morphological and genetic variation in annatto (Bixa orellana L.) by sequence-related amplified polymorphism and cluster analysis. Hort Sci 43:2013–2017Google Scholar
  37. Valdez-Ojeda R, Quiros CF, Aguilar-Espinosa ML, Rivera-Madrid R (2010) Outcrossing rates in annatto determined by sequence-related amplified polymorphism. Agron J 102:1340–1345CrossRefGoogle Scholar
  38. Venkatesh CS (1956) The curious anther of Bixa – its structure and dehiscence. Am Midland Naturalist 55:473–476CrossRefGoogle Scholar
  39. Wilson RF (1938) Wilson colour chart, vol I. Henry Stone, BanburyGoogle Scholar
  40. Wilson RF (1941) Wilson colour chart, vol II. Henry Stone, BanburyGoogle Scholar
  41. Wilson MF (1983) Plant reproductive ecology. Wiley, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of BotanyUniversity of KeralaThiruvananthapuramIndia

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