Single nucleotide mutation leading to an amino acid substitution in the variant Tik soybean Kunitz trypsin inhibitor (SKTI) identified in Chinese wild soybean (Glycine soja Sieb. & Zucc.)
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The wild soybean (Glycine soja), which is the progenitor of cultivated soybean (Glycine max), is expected to offer more information about genetic variability and more useful mutants for evolutionary research and breeding applications. Here, a total of 1,600 wild soybean samples from China were investigated for genetic variation with regard to the soybean Kunitz trypsin inhibitor (SKTI). A new mutant SKTI, Tik, was identified. It was found to be a Tia-derived codominant allele caused by a transversion point mutation from C to G at nucleotide +171, leading to an alteration of one codon (AAC → AAG) and a corresponding amino acid substitution (Asn → Lys) at the ninth residue. Upon examination of this variant and others previously found in wild soybeans, it became clear that SKTI has undergone high-level evolutionary differentiation. There were more abundant polymorphisms in the wild than in the cultivated soybean.
KeywordsKunitz-trypsin inhibitor Protein polymorphism Gene sequence Wild soybean Glycine soja
The work was supported by the National Natural Science Foundation of China (item no. 30771308). Most of the analytical work was completed under the supervision of Prof. Y. Takahata of the Plant Breeding Laboratory at the Iwate University Faculty of Agriculture.
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