Karyotype and AFLP data reveal the phylogenetic position of the Brazilian endemic Hypochaeris catharinensis (Asteraceae)

  • Maikel Reck
  • Lucas M. Benício
  • Eduardo A. Ruas
  • Luana A. Rodrigues
  • Paulo M. Ruas
  • María A. Ortiz
  • Salvador Talavera
  • Estrella Urtubey
  • Tod Stuessy
  • Hanna Weiss-Schneeweiss
  • Karin Tremetsberger
  • Vanessa S. Michelan
  • Nelson I. Matzenbacher
  • André L. L. Vanzela
  • Anass Terrab
  • Rose Samuel
  • Claudete F. Ruas
Original Article
First Online:
Received:
Accepted:

Abstract

The genus Hypochaeris offers an excellent model for studies of recent adaptive radiation in the South American continent. We used karyotype analysis with chromomycin A3 (CMA3)/4’,6-diamidino-2-phenylindole (DAPI) banding and fluorescence in situ hybridization (FISH), and amplified fragment length polymorphism (AFLP) fingerprinting to investigate for the first time the Brazilian endemic H. catharinensis and define its position within the South American group of species. Strong CMA-positive signals were seen at the end of both arms of chromosome 3 and at the end of the long arm of chromosome 4. DAPI bands were only detected in subterminal position on short arm of chromosome 4. FISH with 5S and 35S ribosomal DNA (rDNA) probes revealed a single 5S rDNA locus on short arm of chromosome 2, typical for all other South American Hypochaeris taxa analyzed to date. The 35S rDNA locus was identified at subterminal position on the short arm of chromosome 3, as reported so far for only two of the known species (H. lutea and H. patagonica). The AFLP study included 55 individuals, comprising nine species of the South American Hypochaeris plus their putative ancestor H. angustifolia. Eleven AFLP primer combinations generated a total of 401 fragments, of which 388 (96.7%) were polymorphic. High genetic similarities were observed among taxa, with all South American Hypochaeris species falling into one main cluster [100% bootstrap (BS)]. Hypochaeris catharinensis is closely related to H. lutea (82% BS), forming a well-separated subcluster within the South American species. Taken together, the karyological and AFLP data contribute to the placement of H. catharinensis within the phylogenetic framework of South American species of Hypochaeris and allow the definition of a novel and well-resolved phylogenetic group (the Lutea group).

Keywords

AFLP Asteraceae Cytogenetics FISH Hypochaeris catharinensis Phylogeny 
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Notes

Acknowledgments

We sincerely thank The Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP6510), the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (grants to C.F.R. and P.M.R. CNPQ no. 201254/2003-4 and 201332/2003-5), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Capes, and Fundación BBVA for providing funding.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Maikel Reck
    • 1
  • Lucas M. Benício
    • 1
  • Eduardo A. Ruas
    • 1
  • Luana A. Rodrigues
    • 1
  • Paulo M. Ruas
    • 1
  • María A. Ortiz
    • 2
  • Salvador Talavera
    • 2
  • Estrella Urtubey
    • 3
  • Tod Stuessy
    • 4
  • Hanna Weiss-Schneeweiss
    • 4
  • Karin Tremetsberger
    • 4
    • 5
  • Vanessa S. Michelan
    • 1
  • Nelson I. Matzenbacher
    • 6
  • André L. L. Vanzela
    • 1
  • Anass Terrab
    • 2
  • Rose Samuel
    • 4
  • Claudete F. Ruas
    • 1
  1. 1.Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de Biología Vegetal y EcologíaUniversidad de SevillaSevillaSpain
  3. 3.Instituto de Botánica DarwinionSan IsidroArgentina
  4. 4.Department of Systematic and Evolutionary BotanyUniversity of ViennaViennaAustria
  5. 5.Department of Integrative Biology and Biodiversity Research, Institute of BotanyUniversity of Natural Resources and Life SciencesViennaAustria
  6. 6.Departamento de BotânicaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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