Plant Systematics and Evolution

, Volume 295, Issue 1–4, pp 23–54 | Cite as

Molecular systematics, character evolution, and pollen morphology of Cistus and Halimium (Cistaceae)

  • Laure Civeyrel
  • Julie Leclercq
  • Jean-Pierre Demoly
  • Yannick Agnan
  • Nicolas Quèbre
  • Céline Pélissier
  • Thierry Otto
Original Article

Abstract

Pollen analysis and parsimony-based phylogenetic analyses of the genera Cistus and Halimium, two Mediterranean shrubs typical of Mediterranean vegetation, were undertaken, on the basis of cpDNA sequence data from the trnL-trnF, and trnS-trnG regions, to evaluate limits between the genera. Neither of the two genera examined formed a monophyletic group. Several monophyletic clades were recognized for the ingroup. (1) The “white and whitish pink Cistus”, where most of the Cistus sections were present, with very diverse pollen ornamentations ranging from striato-reticulate to largely reticulate, sometimes with supratectal elements; (2) The “purple pink Cistus” clade grouping all the species with purple pink flowers belonging to the Macrostylia and Cistus sections, with rugulate or microreticulate pollen. Within this clade, the pink-flowered endemic Canarian species formed a monophyletic group, but with weak support. (3) Three Halimium clades were recovered, each with 100% bootstrap support; all Halimium species had striato-reticulate pollen. Two Halimium clades were characterized by yellow flowers, and the other by white flowers.

Keywords

TrnL-F TrnS-G Pollen Exine Cistaceae Cistus Halimium 

Notes

Acknowledgments

We are most grateful to curators or keepers of the herbaria G, K, MPU, and P for permitting the examination of specimens and removal of pollen samples. We should like to thank Peter Biggins from CIRAD for revising the English, and also all colleagues of the palynology unit at the R. B. G. Kew for their assistance, the Conservatoire Botanique National de Brest and Robert Page for their help, and Olivier Filippi for cultivating our plants collected on field trips, especially from Morocco and the Canary Islands. We also wish to thank Elise Van Campo, Laurent Chibret, and Renaud Lahaye for their support.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Laure Civeyrel
    • 1
  • Julie Leclercq
    • 2
  • Jean-Pierre Demoly
    • 3
  • Yannick Agnan
    • 1
  • Nicolas Quèbre
    • 4
  • Céline Pélissier
    • 1
  • Thierry Otto
    • 1
  1. 1.Laboratoire Ecologie Fonctionnelle et EnvironnementUniversité de ToulouseToulouse Cedex 9France
  2. 2.UMR DAP, CIRADMontpellier Cedex 5France
  3. 3.APBFParisFrance
  4. 4.ETH Zentrum, IBZZurichSwitzerland

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