The karyotype of Clivia mirabilis analyzed by differential banding and fluorescence in-situ hybridization
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The karyotype of the recently described species Clivia mirabilis was analyzed by differential chromosome staining with Giemsa, chromomycin, and DAPI and by fluorescence in-situ hybridization with 5S and 45S rDNA probes. Like the other five Clivia species it was shown to have a unique karyotype, although its karyotype was similar in several respects to that of C. gardenii, differing in having only one pair of chromosomes with CMA bands compared with two pairs in C. gardenii and lacking any DAPI-positive bands. The evolutionary relationships of the species and their karyotypes are discussed.
KeywordsClivia mirabilis Chromosomes Differential chromosome banding FISH Karyotypes
We thank Diana Holt for allowing us to collect root tips from her plants of C. mirabilis.
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