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Plant Systematics and Evolution

, Volume 293, Issue 1–4, pp 193–196 | Cite as

The karyotype of Clivia mirabilis analyzed by differential banding and fluorescence in-situ hybridization

  • Brian G. Murray
  • Charles Wong
  • Keith R. W. Hammett
Original Article

Abstract

The karyotype of the recently described species Clivia mirabilis was analyzed by differential chromosome staining with Giemsa, chromomycin, and DAPI and by fluorescence in-situ hybridization with 5S and 45S rDNA probes. Like the other five Clivia species it was shown to have a unique karyotype, although its karyotype was similar in several respects to that of C. gardenii, differing in having only one pair of chromosomes with CMA bands compared with two pairs in C. gardenii and lacking any DAPI-positive bands. The evolutionary relationships of the species and their karyotypes are discussed.

Keywords

Clivia mirabilis Chromosomes Differential chromosome banding FISH Karyotypes 

Notes

Acknowledgments

We thank Diana Holt for allowing us to collect root tips from her plants of C. mirabilis.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Brian G. Murray
    • 1
  • Charles Wong
    • 1
  • Keith R. W. Hammett
    • 2
  1. 1.School of Biological SciencesThe University of AucklandAucklandNew Zealand
  2. 2.AucklandNew Zealand

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