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Plant Systematics and Evolution

, Volume 292, Issue 1–2, pp 85–93 | Cite as

Systematic status and phylogenetic relationships of the enigmatic Tanacetum paradoxum Bornm. (Asteraceae, Anthemideae): evidences from nrDNA ITS, micromorphological, and cytological data

  • Ali SonboliEmail author
  • Shahrokh Kazempour Osaloo
  • Joan Vallès
  • Christoph Oberprieler
Original Article

Abstract

In order to evaluate the phylogenetic relationships and taxonomic classification of the Iranian endemic Tanacetum paradoxum Bornm. (Asteraceae, Anthemideae), a molecular analysis based on nrDNA ITS sequences of 16 species of Tanacetum together with representatives of 27 genera of subtribes Artemisiinae and Handeliinae as well as a micromorphological study of achenes and pollen grains was performed. The chromosome number of 2n = 2x = 16 and the genome size of T. paradoxum (2C = 8.81 ± 0.19 pg) are reported here for the first time. Due to the well-supported close phylogenetic relationship of T. paradoxum to the species of the genus Artemisia (Bayesian inference, posterior probability PP = 1), which is supported by cytological (x = 8) and micromorphological features concerning the achene shape and pollen type, the combination Artemisia paradoxa (Bornm.) Sonboli is proposed.

Keywords

Artemisia Chromosomes Compositae Genome size Iran Phylogeny Tanacetum 

Notes

Acknowledgments

The authors would like to thank M. Asadollahi (Shiraz, Iran) and M. Mozaffarian (Research Institute of Forests and Rangelands, Tehran) for their cooperation in collecting plant material, N. Olanj (Shahid Beheshti University, Iran), S. Ruiz (Universitat de Barcelona, Spain), and P. Hummel (Regensburg University, Germany) for their technical laboratory assistance, T. Garnatje (Institut Botànic de Barcelona) for help with analyses, and J. Martín (Universitat de Barcelona) for comments on palynological aspects. The financial support of this work to A. Sonboli from DAAD (German Academic Exchange Service) and Shahid Beheshti University Research Council and to J. Vallès by projects CGL2010-22234-C02-01 and CGL2010-22234-C02-02 of the Spanish government is gratefully appreciated.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ali Sonboli
    • 1
    Email author
  • Shahrokh Kazempour Osaloo
    • 2
  • Joan Vallès
    • 3
  • Christoph Oberprieler
    • 4
  1. 1.Department of Biology, Medicinal Plants and Drugs Research InstituteShahid Beheshti UniversityTehranIran
  2. 2.Department of Plant Biology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  3. 3.Laboratori de Botànica, Facultat de FarmàciaUniversitat de BarcelonaBarcelonaSpain
  4. 4.Institute of BotanyUniversity of RegensburgRegensburgGermany

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