Inter and intra-population variability of Pongamia pinnata: a bioenergy legume tree
- 218 Downloads
Pongamia pinnata is an oil-producing tree species with multiple uses and considerable potential as a bioenergy crop. This investigation was carried out to assess the extent of genetic structure in a representative set of 226 individuals of Pongamia pinnata encompassing seven populations as a prelude to utilization of promising and genetically divergent material in breeding programmes. Molecular polymorphism was 67.18% with ten inter simple sequence repeats (ISSR) between the individuals indicating modest levels of genetic variation in the Pongamia pinnata germplasm collected. The within-population variation based on ISSR polymorphism was 32.34% and polymorphism at the species level was 94.3%. Genetic differentiation between populations (GST = 0.61) was positively correlated with geographical distance. The data obtained indicate an immediate need to widen the genetic base of Pongamiapinnata germplasm for proper characterization, and for extensive plantations of elite varieties to meet the demands for biodiesel.
KeywordsBioenergy crop ISSR markers Population diversity
The authors wish to acknowledge to Orissa Forest Development Corporation Limited (OFDC), Bhubaneswar, for providing financial assistance to conduct the experiment and survey.
- Burkill JH (1966) A dictionary of economic products of Malaya peninsula L., vol 2. Art Printing Works, Kuala LumpurGoogle Scholar
- Doyle JJ, Doyle DJ (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- Hamrick JL, Loveless MD (1989) The genetic structure of tropical tree populations: association with reproductive biology. In: Bock J, Linhart YB (eds) The evolutionary ecology of plants. Westview Press, Boulder, pp 130–146Google Scholar
- Yeh FC, Chong DKS, Yang RC (1995) RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta. J Heredity 86:454–460Google Scholar
- Yeh FC, Yang RC, Boyle T (1999) POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31. University of Alberta, EdmontonGoogle Scholar