Crystal diversity and macropatterns in leaves of Oleaceae
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Oleaceae leaves surveyed from herbarium specimens of 240 species from 23 genera were rehydrated, bleached, processed into xylol, mounted unstained, and viewed microscopically between crossed polarizers. Occurrence of five crystal types and two variants (tiny acicular crystals and sphaerites) within one family is unusual. Number of crystal types within a single species was one (108 spp.), two (53 spp.), three (51 spp.), four (15 spp.), and five (6 spp.). Seven species lacked crystals. The tiny acicular crystal variant was most common (167 spp.), followed by prisms (67 spp.), raphides (65 spp.), druses (61 spp.), sphaerites variant (50 spp.), styloids (36 spp.), and crystal sand (21 spp.). Epidermal crystals were common (155 spp.), with epidermal crystals clustering at base of trichomes in 21 species. Jasminum was exceptional in having mostly druses and almost no crystals around vascular bundles. Most Oleaceae crystals are tiny, usually about 5 μm in length, except for larger styloids and raphides.
KeywordsAcicular crystals Crystal macropatterns Crystal sand Druses Leaves Oleaceae Prisms Raphides Sphaerites
The authors thank their respective departments for partial support of this research, the staff of the Microscopy and NanoImaging Facility, Iowa State University (ISU), where the work was done, and the directors and curators at Field Museum, Chicago; Missouri Botanical Garden, St. Louis; and Ada Hayden Herbarium (ISU), and curator Deborah Lewis for special assistance, for permission to collect leaf samples from selected herbarium specimens.
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