Plant Systematics and Evolution

, Volume 282, Issue 1–2, pp 57–70 | Cite as

Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis

  • Majid Sharifi Tehrani
  • Mohsen Mardi
  • Jamal Sahebi
  • Pilar Catalán
  • Antonio Díaz-Pérez
Original Article

Abstract

Tall fescue (Festuca arundinacea Schreb. subsp. arundinacea) is one of the most economically important forage grasses in cold and temperate regions of the world. In this study, we have assessed the genetic diversity and structure of wild Iranian tall fescue populations. Thirty-seven individuals from nine natural populations from northern, western, and southern Iranian mountain ranges were analyzed using six genomic-SSRs and five EST-SSRs primer pairs. Our analysis has demonstrated that transcribed EST-SSR regions showed levels of polymorphism similar to genomic-SSR regions. UPGMA, repeated bisection, and model-based Bayesian STRUCTURE clustering methods coupled with neighbor-net network were used to establish six divergent groups of individuals. FST estimates among clusters showed moderate to low genetic structure. Within-group genetic diversity estimate H and partial correlations between genetic and geographic distances among populations suggested that western Zagros population was related to the rest of the Iranian individuals. The isolation-by-distance hypothesis was not supported by SSR data and the present geographical sampling.

Keywords

Consensus groups Genetic variability Iran SSR Tall fescue 

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Majid Sharifi Tehrani
    • 1
  • Mohsen Mardi
    • 2
  • Jamal Sahebi
    • 3
  • Pilar Catalán
    • 4
  • Antonio Díaz-Pérez
    • 4
    • 5
  1. 1.Department of BiologyUniversity of ShahrekordShahrekordIran
  2. 2.Agricultural Biotechnology Research Institute of Iran (ABRII)KarajIran
  3. 3.Department of BiologyUniversity of IsfahanIsfahanIran
  4. 4.High Polytechnic School of HuescaUniversity of ZaragozaHuescaSpain
  5. 5.Facultad de Agronomía, Instituto de GenéticaUniversidad Central de VenezuelaMaracayVenezuela

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