Genetic structure of peripheral, island-like populations: a case study of Saponaria bellidifolia Sm. (Caryophyllaceae) from the Southeastern Carpathians
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Geographically peripheral populations often experience a reduction of genetic diversity and divergence from the core populations. Habitat geometry and quality can induce a local genetic diversity pattern, which overlies the regional variability issued from the range-wide phylogeography. We evaluated the genetic variation and genetic divergence of Saponaria bellidifolia Sm. on limestone outcrops within peripheral island-like populations from the Southeastern Carpathians, using RAPD markers. We also determined the degree of isolation related to other European populations, using AFLP. The Romanian populations had a decreased overall genetic diversity shared among populations, with lower level in small populations. Potential habitat size had a positive effect on genetic diversity estimates. Fisher’s exact tests of genetic differentiation revealed significant divergences only between the geographically most distant populations. Romanian populations were genetically pauperised as compared to Bulgarian and Italian populations and our results suggest that they might have originated from a recent range expansion from southern glacial refugia.
KeywordsGenetic diversity Naturally fragmented habitats Peripheral populations Apuseni Mountains Romania Saponaria bellidifolia RAPD AFLP
We are grateful to the team of the Department of Genetics and Plant Breeding from Corvinus University of Budapest, especially Bacskainé Papp Anna, as well as to Lányi Szabolcs from Sapientia Hungarian University of Transylvania and Somogyi Gabriella from the Department of Botany, Corvinus University of Budapest for their help with laboratory works. We express our gratitude to the anonymous reviewers, whose comments have considerably improved the manuscript. Plant material was kindly provided by Gérard Largier, Jocelyne Cambecèdes and Delphine Fallour (France), Marija Edita-Šolić (Croatia), Fabio Conti (Italy) and Vladimir Vladimirov (Bulgaria). Research was part of Csergő Anna-Maria’s Ph.D. thesis and was partially supported by Domus Hungarica Scientiarum et Artium, Hungary.
- Boşcaiu N (1971) Flora şi vegetaţia Munţilor Ţarcu, Godeanu şi Cernei. Acad. R.S.R., BucureştiGoogle Scholar
- Boşcaiu N, Marossy A (1980–1981) Interferenţe fitogeografice din Munţii Apuseni. Nymphaea 8–9:395–400Google Scholar
- Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50Google Scholar
- Holt RD (2003) On the evolutionary ecology of species’ ranges. Evol Ecol Res 5:159–178Google Scholar
- Jalas J, Suominen J (eds) (1986) Atlas Florae Europaeae. Distribution of vascular plants in Europe, vol 7. Caryophyllaceae (Silenoideae). The Committee for Mapping the Flora of Europe & Societas Biologica Fennica Vanamo, HelsinkiGoogle Scholar
- Miller MP (1997) Tools for population genetic analyses (TFPGA) 1.3: a Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by the authorGoogle Scholar
- R Development Core Team (2007) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
- Sokal R, Rohlf FJ (1995) Biometry. W.H. Freeman and Co., New YorkGoogle Scholar
- Tero N, Aspi J, Siikamäki P, Jäkäläniemi A (2005) Local genetic population structure in an endangered plant species, Silene tatarica (Caryophyllaceae). Heredity 1–10Google Scholar
- Wróblewska A, Brzosko E (2006) The genetic structure of the steppe plant Iris aphylla L. at the northern limit of its geographical range. Bot J Linn Soc 152:245–255Google Scholar