Plant Systematics and Evolution

, Volume 277, Issue 1–2, pp 13–20

Mating system and genetic diversity in natural populations of Nelumbo nucifera (Nelumbonaceae) detected by ISSR markers

Original Article

DOI: 10.1007/s00606-008-0096-x

Cite this article as:
Han, YC., Teng, CZ., Wahiti, G.R. et al. Plant Syst Evol (2009) 277: 13. doi:10.1007/s00606-008-0096-x


In this study, outcrossing rates and genetic diversity in natural populations of Nelumbo nucifera were investigated. The estimated multilocus outcrossing rate (tm) based on 28 ISSR loci was over 90%. Analysis of genetic diversity revealed that this index was high at the species level (Hs = 0.325, I = 0.514), but low within the individual study populations (Hs = 0.148, I = 0.212). Gst-B was 0.547 and Nm was 0.414. The results of AMOVA indicated that 54.6% of the variation was due to the difference between the regions and 45.4% to the variation within the region. Although the populations were predominantly outcrossing, most of the genetic diversity was attributed to geographical effects instead of their habitats because low sexual recruitment and clonal growth deeply reduced the genetic diversity within the populations. On the basis of the high tm, Gst-B and low Nm values, we recommended that any future conservation plans should include both in situ conservation and germplasm collection.


Genetic diversity Genetic structure ISSR Outcrossing rate Nelumbo nucifera 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Yan-Chuang Han
    • 1
  • Cai-Zhu Teng
    • 2
  • Gituru Robert Wahiti
    • 3
  • Ming-Quan Zhou
    • 4
  • Zhong-Li Hu
    • 1
  • Yun-Chun Song
    • 1
  1. 1.Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life ScienceWuhan UniversityWuhanChina
  2. 2.College of Life ScienceGuangxi Normal UniversityGuilinChina
  3. 3.Botany DepartmentJomo Kenyatta University of Agriculture and TechnologyNairobiKenya
  4. 4.Lotus CenterWuhan UniversityWuhanChina

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