Advertisement

Plant Systematics and Evolution

, Volume 276, Issue 1–2, pp 105–123 | Cite as

A palynological study of the genus Nepeta L. (Lamiaceae)

  • Sevcan CelenkEmail author
  • Tuncay Dirmenci
  • Hulusi Malyer
  • Adem Bicakci
Original Article

Abstract

The pollen morphology of forty taxa of the genus Nepeta L. was studied and documented in detail using light microscopy (LM) and scanning electron (SEM) microscopy in this study. Nepeta L. pollen grains are small to large (P = 18.64–63.46 μm, E = 15.62–46.33 μm), suboblate to perprolate (P/E = 0.86–2.09) in shape and hexacolpate (very rarely tetracolpate) with granular membranes. Alternate position of colpi occurs in six Nepeta taxa, N. nuda ssp. glandulifera, N. concolor, N. crinita, N. congesta var. cryptantha, N. stricta var. stricta and N. sibthorpii ssp. tumeniana. In examinations of exine ornamentation with SEM, two types of pollen grains were recognized: (1) type I, with microreticulate sculpture; and (2) type II with bireticulate sculpture, type I and II to be divided into two and five subtypes, respectively. In the two taxa with microreticulate pattern, N. pilinux and N. sulfuriflora, a tendency towards a bireticulum could be recognized due to traces of secondary tectal connections. The bireticulate exine ornamentation is characterized with varying characteristics of the primary muri and secondary reticulum. Pollen morphology within the genus is compared with infrageneric relationships.

Keywords

Infrageneric classification Lamiaceae LM Nepeta Pollen morphology SEM 

Notes

Acknowledgments

This study is the Ph.D. thesis of the first author and supported by the Uludag University Research Foundation Centre (project number: 2001/62).

References

  1. Abu-Asab MS, Cantino PD (1992) Pollen morphology in the subfamily Lamioideae (Labiatae) and its phylogenetic implications. In: Harley RM, Reynolds T (eds) Advances in Labiatae science. Royal Botanical Gardens, Kew, London, pp 97–112Google Scholar
  2. Abu-Asab MS, Cantino PD (1994) Systematic implications of pollen morphology in subfamilies Lamioideae and Pogostemonoideae (Labiatae). Ann Missouri Bot Gard 81:653–686CrossRefGoogle Scholar
  3. Azizian DZ, Jamzad Z, Serpooshan F (2001) Pollen morphology and taxonomy of the genus Nepeta sect. Psilonepeta (Labiatae) in Iran. Iran J Bot 9(1):19–26Google Scholar
  4. Baser KHC, Kirimer N, Kurkcuoglu M, Demirci B (2000) Essential oil of Nepeta species growing in Turkey. Chem Nat Compd 36(4):356–359CrossRefGoogle Scholar
  5. Boissier E (1879) Flora orientalis, vol 4(2). Geneve, pp 629–670Google Scholar
  6. Budantsev AL (1997) Fruit morphology, anatomy and taxonomy of tribe Nepeteae (Lamiaceae). Edinburgh J Bot 54(2):183–216CrossRefGoogle Scholar
  7. Cantino PD, Sanders RW (1986) Subfamilial classification of Labiatae. Syst Bot 11:163–185CrossRefGoogle Scholar
  8. Cantino PD, Harley RM, Wagstaff SJ (1992) Genera of Lamiaceae: status and classification. In: Harley RM, Reynolds T (eds) Advances in Labiatae science. Royal Botanic Gardens, Kew, London, pp 511–523Google Scholar
  9. Celenk S, Tarimcilar G, Bicakci A, Kaynak G, Malyer H (2008) A palynological study of Mentha L. Bot J Linn Soc 157:141–154CrossRefGoogle Scholar
  10. Dirmenci T (2003) Taxonomic investigations on Nepeta L. (Lamiaceae) species growing in Turkey. Ph.D. thesis, Univ Balikesir, TurkeyGoogle Scholar
  11. Dirmenci T (2005) A new subspecies of Nepeta (Lamiaceae) from Turkey. Bot J Linn Soc 147:229–233CrossRefGoogle Scholar
  12. Erdtman G (1945) Pollen morphology and plant taxonomy. IV. Labiatae, Verbenaceae, and Aviceniaceae. Svensk Bot Tidskr 39:279–285Google Scholar
  13. Erdtman G (1960) The acetolysis method: a revised description. Svensk Bot Tidskr 54:561–564Google Scholar
  14. Faegri K, Iversen J (1975) Textbook of pollen analysis. Hafner Press, New YorkGoogle Scholar
  15. Harley MM (1992) The potential value of pollen morphology as an additional taxonomic character in subtribe Ociminae (Ocimeae: Nepetoideae: Labiatae). In: Harley RM, Reynolds T (eds) Advances in Labiate science. Royal Botanical Gardens, Kew, London, pp 125–138Google Scholar
  16. Harley MM, Paton A, Harley RM, Cade PG (1992) Pollen morphological studies in tribe Ocimeae (Nepetoideae: Labiatae): I. Ocimum L. Grana 31:161–176CrossRefGoogle Scholar
  17. Hedge L, Lamond J (1982) Flora of Turkey and East Aegean islands, vol 7. Edinb. Univ. Press, Edinburgh, pp 264–288Google Scholar
  18. Hedge L (1986) Lamiaceae of South-West Asia: diversity, distribution and endemism. In: Proceeding of the Royal Society, 89b, Edinburgh, pp 23–25Google Scholar
  19. Husain SZ, Heywood VH (1982) Pollen morphology of the genus Origanum L. and allied genera. In: Margaris N, Koedam A, Vokou D (eds) Aromatic plants: basic and applied aspects. Martinus Nijhof, The Hague, pp 39–56Google Scholar
  20. Jamzad Z, Harley MM, Ingrouille M, Simmonds MSJ, Jalili A (2000) Pollen exine and nutlet surface morphology of the annual species of Nepeta L. (Lamiaceae) in Iran. In: Harley MM, Morton GM, Blackmore S (eds) Pollen and spores: morphology and biology. Royal Botanic Garden, Kew, London, pp 385–397Google Scholar
  21. Jamzad Z, Ingrouille M, Simmonds MSJ (2003a) Three new species of Nepeta (Lamiaceae) from Iran. Taxon 52:93–98CrossRefGoogle Scholar
  22. Jamzad Z, Chase WM, Ingrouille M, Simmonds MSJ, Jalili A (2003b) Phylogenetic relationship in Nepeta L. (Lamiaceae) and related genera based on its sequence data. Taxon 52:21–32CrossRefGoogle Scholar
  23. Moon HK, Hong SP (2003) Pollen morphology of the genus Lycopus (Lamiaceae). Ann Bot Fenn 40:191–198Google Scholar
  24. Moon HK, Vinckier S, Smets E, Huysmans S (2008) Comparative pollen morphology and ultrastructure of Menthae subtribe Nepetinae (Lamiaceae). Rev Palaeobot Palynol 149:174–186CrossRefGoogle Scholar
  25. Morales-Valverde R (1987) El genero Thymbra L. (Labiatae). Anales Jard Bot Madr 44:349–380Google Scholar
  26. Morales-Valverde R (1990) El genero Micromeria Bentham (Labiatae) En La Peninsula Iberica Islas Baleares. Anales Jard Bot Madr 48:131–156Google Scholar
  27. Newall CA, Anderson LA, Phillipson JD (1996) Herbal medicines, a guide for health-care professionals. The Pharmaceutical Press, London, p 154Google Scholar
  28. Paton AJ, Harley MR, Harley MM, Weeks S (1994) A revision of Endostemon (Labiatae). Kew Bull 49(4):673–716CrossRefGoogle Scholar
  29. Perveen A, Qaiser M (2003) Pollen morphology of the family Labiatae from Pakistan. Pak J Bot 35(5):671–693Google Scholar
  30. Pojarkova AI (1954) Nepeta L.: 191–293. In: Shishkin BK (ed) Flora of the USSR, vol 20. Academy Science of the USSR, Moskva-LeningradGoogle Scholar
  31. Pozhidaev A (1992) The origin of three- and sixcolpate pollen grains in the Lamiaceae. Grana 31:49–52Google Scholar
  32. Punt W, Hoen PP, Blackmore S, Nilsson S, Le Thomas A (2007) Glossary of pollen and spore terminology. Rev Palaeobot Palynol 143:1–81CrossRefGoogle Scholar
  33. Ranjbari HF, Nejadi HD, Jamzad Z (2004) Palynological study of Section Cataria of genus Nepeta In Iran. In: The joint agriculture and natural resources symposium, Tabriz-Ganja, May 14–16, 2004Google Scholar
  34. Rechinger KH (1982) Flora Iranica, Akademische Druck-U. Verlagsanstalt, GrazGoogle Scholar
  35. Sammataro D, Avitabile A (1998) The Beekeper’s handbook. Comstock Publishing Associates, Ithaca, p 8Google Scholar
  36. Schols P, Dessein S, D’Hondt C, Huysmans S, Smets E (2002) Carnoy: a new digital measurement tool for palynology. Grana 41:124–126CrossRefGoogle Scholar
  37. Shishkin B (1976) Flora of the Ussr, Izdatel’stvo Akademii Nauk Ussr, Moskva-Leningrad (1954) Translated from Russian Israel program for scientific translations. Jerusalem 21:427–449Google Scholar
  38. Ubera JL (1982) Estudio palinologico del genero Nepeta L. en la Peninsula Iberica. Macaroriesica 8–9:215–230Google Scholar
  39. Wagstaff S (1992) A phylogenetic interpretation of pollen morphology in Tribe Mentheae (Labiatae). In: Harley RM, Reynolds T (eds) Advances in Labiatae science. Royal Botanic Gardens, Kew, London, pp 113–124Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sevcan Celenk
    • 1
    Email author
  • Tuncay Dirmenci
    • 2
  • Hulusi Malyer
    • 1
  • Adem Bicakci
    • 1
  1. 1.Art and Science Faculty, Biology DepartmentUludag UniversityGorukle, BursaTurkey
  2. 2.Necatibey Education Faculty, Department of Biology EducationBalikesir UniversityBalikesirTurkey

Personalised recommendations