Single nucleotide polymorphisms of Gcyc1 (Cycloidea) in Conandron ramondioides (Gesneriaceae) from Southeast China
- 76 Downloads
Conandron ramondioides with actinomorphic flower in Gesneriaceae is an endemic species distributed in Taiwan, Southeast of China and Japan. Populations are usually small and isolated in typically fragmented habitat. Based on SNPs of Gcyc1 (Cycloidea), a TCP gene known in patterning the floral dorsoventral asymmetry, we have explored the molecular evolution and genetic differentiation of Gcyc1 at population level, and the population history of C. ramondioides populations distributed in SE China. Eighteen SNPs are detected in 774-bp of the gene, of which eleven are non-synonymous. However, morphological observation of flowers shows that there is no visible differentiation in shape and size across the dorsoventral axis within each whorl. None of the eighteen SNPs is by all shared the eleven populations. Population differentiation is significant. These results reveal that evolution of Gcyc1 at population level is well in accord with the neutral theory. Our study indicates that the SNPs of developmental genes are also useful molecular markers for exploring the genetic differentiation and population history in non-model organisms.
KeywordsConandron ramondioides (Gesneriaceae) Gcyc1 gene Habitat fragmentation Non-model organism Population history SNP
Unable to display preview. Download preview PDF.
- Barrett SCH, Kohn JR (1991) Genetic and evolutionary consequences of small population size in plants: implication for convervation. In: Falk D. A. Genetic, conservation of rare plants. Blackwell Science (ed.), Oxford University Press, New York, Oxford, pp 3–30Google Scholar
- Doyle JJ and Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15 Google Scholar
- Fore AF and Guttman SI (1992). Genetic structure after forest fragmentation: a landscape ecology perspective of Acer saccharum. Canad J Bot 70: 1659–1668 Google Scholar
- Hamrick JK and Godt MJW (1990). Allozyme diversity in plant species. In: Brown, AHD, Clegg, MT and Kahler, AL et al. (eds) Plant population genetics, breeding, and genetic resources, pp 43–63. Mass. Sinauer, Sunderland Google Scholar
- Hanski I (1991). Metapopulation dynamics: brief history and conceptual domain. Biol J Linn Soc 42: 3–16 Google Scholar
- Kosugi S and Ohashi Y (1997). PCF1 and PCF2 specially bind to cis elements in the rice proliferating cell nuclear antigen gene. Pl Cell 9: 1607–1619 Google Scholar
- Möller NJ (1999). Integrating molecular phylogenies and developmental genetics: a Gesneriaceae case study. Chapter 17: 395–402 Google Scholar
- Nasu S, Suzuki J, Ohta R, Hasegawa K, Yui R, Kitazawa N and Minobe Y (2002). Search for and analysis of single nucleotide polymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNP markers. DNA Research Int J Rapid Publ Reports Genes Genomes 9: 163–171 Google Scholar