Plant Systematics and Evolution

, Volume 269, Issue 1–2, pp 15–28 | Cite as

Variability and structure of natural populations of Hordelymus europaeus (L.) Jess. ex Harz and Leymus arenarius (L.) Hochst. as revealed by morphology and DNA markers

  • M. Mizianty
  • L. Frey
  • W. Bieniek
  • P. Boroń
  • M. Szklarczyk
Article

Abstract

Seven populations of Hordelymus europaeus and four populations of Leymus arenarius from Poland were subjected to examination of 36 morphological characters. This study showed that both species are relatively uniform and that morphological variation of their populations represents a continuum. Of those, three populations of either species were selected for analysis with molecular markers – RAPDs and AFLPs. These populations differed with respect of geographical location as well as syntaxa and habitat. RAPD-PCR was performed for individual plants and clearly grouped them according to the population origin. For either H. europaeus or L. arenarius - the studied populations differed in degree of their intrinsic variation while none of them as a whole was significantly different from the remaining ones. In AFLP analysis the studied populations were represented by DNA pools of several individual plants. Also this approach allowed discrimination among the population samples of both H. europaeus and L. arenarius. Both RAPDs and AFLPs were accordant in indication that H. europaeus exceeds L. arenarius with respect to variation accumulated at the DNA level.

Keywords

Wild Triticeae Hordelymus europaeus Leymus arenarius morphology AFLP RAPD Poland 

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Copyright information

© Springer 2007

Authors and Affiliations

  • M. Mizianty
    • 1
  • L. Frey
    • 1
  • W. Bieniek
    • 2
  • P. Boroń
    • 3
  • M. Szklarczyk
    • 3
  1. 1.W. Szafer Institute of BotanyPolish Academy of SciencesKrakówPoland
  2. 2.International Doctoral Studies in Natural Sciences at the Polish Academy of Sciences in Kraków, attached to W. Szafer Institute of BotanyPolish Academy of SciencesKrakówPoland
  3. 3.Department of Genetics, Plant Breeding and Seed ScienceAgricultural University of KrakówKrakówPoland

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