Plant Systematics and Evolution

, Volume 264, Issue 1–2, pp 117–134 | Cite as

Genome size variation from a phylogenetic perspective in the genus Cheirolophus Cass. (Asteraceae): biogeographic implications

  • T. GarnatjeEmail author
  • S. Garcia
  • M. Á. Canela


Nuclear DNA amount has been assessed in 21 populations of 19 species of the genus Cheirolophus, and phylogenetic analyses have been performed in order to find the relationships between the species of the Mediterranean Basin and those of Macaronesia. A combined analysis of nuclear ribosomal DNA ITS and ETS has been performed based on Bayesian inference, and nuclear DNA amount has been assessed by flow cytometry. The monophyly of the Macaronesian group and its relationship with Ch. sempervirens indicate a unique colonization event of Macaronesia from the continent. This single event has not been proved in the case of the Canarian archipelago. DNA amount is significantly lower in the insular species than in the continental ones, and a connection appears between the evolution of the genus Cheirolophus (colonization of islands) and the loss of DNA, probably due to the selection pressure existing on the oceanic islands.


Asteraceae biogeography Cheirolophus C-value DNA amount genome size islands molecular phylogeny 


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  1. Albach, D. C., Greilhuber, J. 2004Genome size variation and evolution in Veronica Ann. Bot.94897911PubMedCrossRefGoogle Scholar
  2. Baldwin, B. G., Crawford, D. J., Francisco-Ortega, J., Kim, S. C., Sang, T., Stuessy, T. F. 1998Molecular phylogenetic insights on the origin and evolution of oceanic island plantsSoltis, D. E.Soltis, P. S.Doyle, J. J. eds. Molecular systematics of plants II: DNA sequencingKluwerMassachussets, USA410441Google Scholar
  3. Bancheva, S., Greilhuber, J. 2006Genome size in Bulgarian Centaurea s.l. (Asteraceae)Pl. Syst. Evol.25795117CrossRefGoogle Scholar
  4. Barber, J. C., Francisco-Ortega, J., Santos-Guerra, A., Marrero, A., Jansen, R. K. 2000Evolution of endemic Sideritis (Lamiaceae) in Macaronesia: Insights from a chloroplast DNA restriction site analysisSyst. Bot.25633647CrossRefGoogle Scholar
  5. Barber, J. C., Francisco-Ortega, J., Santos-Guerra, A., Turner, K. G., Jansen, R. K. 2002Origin of Macaronesian Sideritis L. (Lamioideae: Lamiaceae) inferred from nuclear and chloroplast sequence datasetsMolec. Phylogenet. Evol.23293306PubMedCrossRefGoogle Scholar
  6. Barret, S. C. H. 1996The reproductive biology and genetics of island plantsPhilos. Trans., Ser. B351725733CrossRefGoogle Scholar
  7. Bennett, M. D. 1998Plant genome values: How much do we know?Proc. Natl. Acad. U.S.A.9520112016CrossRefGoogle Scholar
  8. Bennett, M. D., Leitch, I. 2005Genome size evolution in plantsGregory, T. R. eds. The evolution of the genomeElsevier Academic PressUSA90151Google Scholar
  9. Bennetzen, J. L., Kellogg, E. A. 1997Do plants have a one way ticket to genomic obesity?Pl. Cell915091514CrossRefGoogle Scholar
  10. Bharathan, G. 1996Reproductive development and nuclear DNA content in angiospermsAmer. J. Bot.83440451CrossRefGoogle Scholar
  11. Böhle, U. R., Hilger, H. H., Martin, W. F. 1996Island colonization and evolution of the insular woody habit in Echium L. (Boraginaceae)Proc. Natl. Acad. U.S.A.931174011745CrossRefGoogle Scholar
  12. Boscaiu M., Riera J., Estrelles E., Güemes J. (1998) Números cromosomáticos de plantas occidentales. 786–808. Anales Jard. Bot. Madrid 56: 119–120.Google Scholar
  13. Bramwell, D. 1972Endemism in the flora of the Canary IslandsValentine, D. H. eds. Taxonomy, phytogeography, and evolutionAcademic PressLondon, UK141159Google Scholar
  14. Bramwell, D. 1976VI. The endemic flora of the Canary Islands; distribution, relationships, and phytogeographyKunkel, G. eds. Biogeography and ecology in the Canary IslandsDr. W. Junk b.v. PublishersThe Hague, The Netherlands207240Google Scholar
  15. Bramwell, D. 1986Contribución a la Biogeografía de las Islas CanariasBot. Macaronés., IV Ci.14334Google Scholar
  16. Bramwell D., Bramwell Z. I. (2001) Wild flowers of the Canary Islands. Rueda, Madrid, Spain.Google Scholar
  17. Bremer, K. 1994Asteraceae. Cladistics and classificationTimberPortland, USAGoogle Scholar
  18. Carlquist, S. 1974Island BiologyColumbia University PressNew York, USAGoogle Scholar
  19. Carine, M. A., Russell, S. J., Santos-Guerra, A., Francisco-Ortega, J. 2004Relationships of the Macaronesian and Mediterranean Floras: Molecular evidence for multiple colonizations into Macaronesia and back-colonization of the continent in Convolvulus (Convolvulaceae)Amer. J. Bot.9110701085Google Scholar
  20. Carracedo, J. C. 1994The Canary Islands: an example of structural control on the growth of large oceanic-island volcanoesJ. Volc. Geot. Res.60225241CrossRefGoogle Scholar
  21. Cassini H. (1817) Cheirolophus. In: Cuvier (ed.) Dictionnaire de Sciences Naturelles 1, Paris, France, pp. 250.Google Scholar
  22. Cerbah, M., Coulaud, J., Brown, S. C., Siljak-Yakovlev, S. 1999Evolutionary DNA variation in the genus Hypochaeris Heredity82261266PubMedCrossRefGoogle Scholar
  23. Crawford, D. J., Whitkus, R., Stuessy, T. F. 1987Plant evolution and speciation on oceanic islandsUrbanska, K. M. eds. Differentiation patterns in higher plantsAcademic PressLondon, UK183189Google Scholar
  24. Dolezel, J., Bartos, J., Voglmayr, H., Greilhuber, J. 2003Nuclear DNA content and genome size of trout and humanCytometry51A127128CrossRefGoogle Scholar
  25. Dostál, J.,  et al. 1976CheirolophusTutin, T. G.Heywood, V. H.Burges, N. A.Moore, D. M.Valentine, D. H.Walters, S. M. eds. Flora EuropaeaCambridge University PressCambridge, UK249250Google Scholar
  26. Fernandopullé, D. 1976Climatic characteristics of the Canary IslandsKunkel, G. eds. Biogeography and ecology in the Canary IslandsDr. W. Junk b.v. PublishersThe Hague, The Netherlands18251826Google Scholar
  27. Francisco-Ortega, J., Jansen, R. K., Santos-Guerra, A. 1996Chloroplast DNA evidence of colonization, adaptive radiation, and hybridization in the evolution of the Macaronesian floraProc. Natl. Acad. U.S.A.9340854090CrossRefGoogle Scholar
  28. Francisco-Ortega, J., Santos-Guerra, A., Hines, A., Jansen, R. K. 1997Molecular evidence for a Mediterranean origin of the Macaronesian endemic genus Argyranthemum (Asteraceae)Amer. J. Bot.8415951613CrossRefGoogle Scholar
  29. Francisco-Ortega, J., Goertzen, L. R., Santos-Guerra, A., Benabid, A., Jansen, R. K. 1999Molecular systematics of the Asteriscus alliance (Asteraceae: Inuleae) I: Evidence from the internal transcribed spacers of nuclear ribosomal DNASyst. Bot.24249266CrossRefGoogle Scholar
  30. Francisco-Ortega, J., Fuertes-Aguilar, J., Kim, S. C., Santos-Guerra, A., Crawford, D. J., Jansen, R. K. 2002Phylogeny of the Macaronesian endemic Crambe section Dendrocrambe (Brassicaceae) based on internal transcribed spacer sequences of nuclear ribosomal DNAAmer. J. Bot.8919841990Google Scholar
  31. Fuertes-Aguilar, J., Ray, M. F., Francisco-Ortega, J., Santos-Guerra, A., Jansen, R. K. 2002Molecular evidence from chloroplast and nuclear markers for multiple colonizations of Lavatera (Malvaceae) in the Canary IslandsSyst. Bot.277483Google Scholar
  32. Galbraith, D. W., Harkins, K. R., Maddox, J. M., Ayres, N. M., Sharma, D. P., Firoozabadi, E. 1983Rapid flow cytometric analysis of the cell cycle in intact plant tissuesScience22010491051CrossRefGoogle Scholar
  33. Garcia, S., Sanz, M., Garnatje, T., Kreitschitz, A., McArthur, E. D., Vallès, J. 2004Variation of DNA amount of 47 populations of the Subtribe Artemis iinae and related taxa (Asteraceae, Anthemideae): Karyological, ecological and systematic implicationsGenome4710041014PubMedCrossRefGoogle Scholar
  34. Garcia-Jacas, N., Susanna, A., Garnatje, T., Susanna, S. 2001Generic delimitation and phylogeny of the subtribe Centaureinae (Asteraceae): A combined nuclear and chloroplast DNA analysisAnn. Bot.87503515CrossRefGoogle Scholar
  35. García-Talavera F. (1999) La Macaronesia. Consideraciones geológicas, biogeográficas y paleoecológicas. In: Fernández-Palacios J. M., Bacallado J. J., Belmonte J. A. (eds.) Ecología y cultura en Canarias. Museo de la Ciencia, Cabildo Insular de Tenerife, Santa Cruz de Tenerife, Canary Islands, Spain, pp. 39–63.Google Scholar
  36. Garnatje T. (1995) Relació entre el polimorfisme enzimàtic i alguns aspectes de l'especiació i de l'evolució en el gènere Cheirolophus Cass. Doctoral Dissertation, Universitat Autònoma de Barcelona.Google Scholar
  37. Garnatje, T., Susanna, A., Messeguer, R. 1998Isozyme studies in the genus Cheirolophus Cass. (Asteraceae: Cardueae-Centaureinae) in the Iberian PeninsulaPl. Syst. Evol.2135770CrossRefGoogle Scholar
  38. Garnatje, T., Vallès, J., Garcia, S., Hidalgo, O., Sanz, M., Canela, M. A., Siljak-Yakovlev, S. 2004Genome size in Echinops L. and related genera (Asteraceae, Cardueae): karyological, ecological and phylogenetic implicationsBiol. Cell96117124PubMedCrossRefGoogle Scholar
  39. Garnatje, T., Garcia, S., Vilatersana, R., Vallès, J. 2006Genome size variation in the genus Carthamus L. (Asteraceae, Cardueae): systematic implications and additive changes during allopolyploidization in hybrid taxaAnn. Bot.97461467PubMedCrossRefGoogle Scholar
  40. Gaut, B. S. 2002Evolutionary dynamics of grass genomesNew Phytol.1541528CrossRefGoogle Scholar
  41. Givnish, T. J. 1998Adaptive plant evolution on islands: classical patterns, molecular data, new islandsGrant, P. R. eds. Evolution on IslandsOxford University PressOxford, UK281304Google Scholar
  42. González M. N. (1992) Historia de la investigación botánica en Canarias. In: Kunkel G. (ed.) Flora y vegetación del archipiélago canario. Tratado florístico 1a parte. Edirca, Las Palmas de Gran Canaria, España.Google Scholar
  43. Govindaraju, D. F., Cullis, C. A. 1991Modulation of genome size in plants: the influence of breeding systems and neighbourhood sizeEvol. Trends Pl.54351Google Scholar
  44. Gregory, T. R. 2001Coincidence, coevolution or causation? DNA content, cell size, and the C-value enigmaBiol. Rev.7665101PubMedCrossRefGoogle Scholar
  45. Gregory, T. R. 2005The C-value enigma in plants and animals: a review of parallels and appeal for partnershipAnn. Bot.95133146PubMedCrossRefGoogle Scholar
  46. Greilhuber, J, Dolezel, J, Lysák, M.A., Bennett, M.D. 2005The origin, evolution and proposed stabilization of the terms ``genome size'' and ``C-value'' to describe nuclear DNA contentsAnn. Bot.95255260PubMedCrossRefGoogle Scholar
  47. Helfgott, D. M., Francisco-Ortega, J., Santos-Guerra, A., Jansen, R. K., Simpson, B. B. 2000Biogeography and breeding system evolution of the woody Bencomia alliance (Rosaceae) in Macaronesia based on ITS sequence dataSyst. Bot.258297CrossRefGoogle Scholar
  48. Hellwig, F. H. 2004Centaureinae (Asteraceae) in the Mediterranean - history of ecogeographical radiationPl. Syst. Evol.246137162Google Scholar
  49. Hidalgo, O., Garcia-Jacas, N., Garnatje, T., Susanna, A. 2006Phylogeny of Rhaponticum (Asteraceae, Cardueae-Centaureinae) and related genera inferred from nuclear and chloroplast DNA sequence data: taxonomic and biogeographic implicationsAnn. Bot.97705714PubMedCrossRefGoogle Scholar
  50. Huelsenbeck, J. P., Ronquist, F. 2001MrBayes: Bayesian inference of phylogenetic treesBioinformatics17754755PubMedCrossRefGoogle Scholar
  51. Juan, C., Emerson, B. C., Oromí, P., Hewitt, G. M. 2000Colonization and diversification: towards a phylogeographic synthesis for the Canary IslandsTrends Ecol. Evol.15104109PubMedCrossRefGoogle Scholar
  52. Kim, S. C., Crawford, D. J., Francisco-Ortega, J., Santos-Guerra, A. 1996A common origin for woody Sonchus and 5 related genera in the Macaronesian islands-molecular evidence for extensive radiationProc. Natl. Acad. U.S.A.9377437748CrossRefGoogle Scholar
  53. Kellogg, E. A. 1998Relationships of cereal crops and other grassesProc. Natl. Acad. U.S.A.9520052010CrossRefGoogle Scholar
  54. Leitch, I. J., Chase, M. W., Bennett, M. D. 1998Phylogenetic analysis of DNA C-values provides evidence for a small ancestral genome size in flowering plantsAnn. Bot.828594CrossRefGoogle Scholar
  55. Linder, C. R., Goertzen, L. R., Heuvel, B. V., Francisco-Ortega, J., Jansen, R. K. 2000The complete external transcribed spacer of 18S–26S rDNA: Amplification and phylogenetic utility at low taxonomic levels in Asteraceae and closely allied familiesMolec. Phylogenet. Evol.14285303PubMedCrossRefGoogle Scholar
  56. Lysák, M. A., Dolezelová, M., Holly, J. P., Swennen, R., Dolezel, J. 1999Flow cytometric analysis of nuclear DNA content in Musa Theor. Appl. Genet.9813441350CrossRefGoogle Scholar
  57. Mes, T. H. M., Hart, H. T. 1996The evolution of growth-forms in the Macaronesian genus Aeonium (Crassulaceae) inferred from chloroplast DNA RFLPs and morphologyMolec. Ecol.5351363CrossRefGoogle Scholar
  58. Mort, M. E., Soltis, D. E., Soltis, P. S., Francisco-Ortega, J., Santos-Guerra, A. 2002Phylogenetics and evolution of the Macaronesian clade of Crassulaceae inferred from nuclear and chloroplast sequence dataSyst. Bot.27271288Google Scholar
  59. Mort, M. E., Crawford, D. J., Santos-Guerra, A., Francisco-Ortega, J., Esselman, E. J., Wolfe, A. D. 2003Relationships among the Macaronesian members of Tolpis (Asteraceae: Lactuceae) based upon analyses of inter simple sequence repeat (ISSR) markersTaxon52511518CrossRefGoogle Scholar
  60. Muñoz, J., Felicísimo, A. M., Cabezas, F., Bergaz, A. R., Martínez, I. 2004Wind a long-distance dispersal vehicle in the southern hemisphereScience30411441147PubMedCrossRefGoogle Scholar
  61. Nylander J. A. (2004) MrModeltest v. 2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.Google Scholar
  62. Olivares, A., Peris, J. B., Stübing, G., Martín, J. 1995 Cheirolophus lagunae, sp. nov. (Asteraceae), endemismo iberolevantinoAnales Jard. Bot. Madrid53262265Google Scholar
  63. Ohri, D. 1998Genome size variation and plant systematicsAnn. Bot. Suppl. A827583CrossRefGoogle Scholar
  64. Pagel, M., Johnstone, R. A. 1992Variation across species in the size of the nuclear genome supports the junk-DNA explanation for tha C-value paradoxProc. Roy. Soc. London, Ser. B. Biol. Sci.249119124CrossRefGoogle Scholar
  65. Panero, J. L., Francisco-Ortega, J., Jansen, R. K., Santos-Guerra, A. 1999Molecular evidence for multiple origins of woodiness and a New World biogeographic connection of the Macaronesian Island endemic Pericallis (Asteraceae: Senecioneae)Proc. Natl. Acad. U.S.A.961388613891CrossRefGoogle Scholar
  66. Percy, D. M., Cronk, Q. C. B. 2002Different fates of island brooms: contrasting evolution in Adenocarpus, Genista, and Teline (Genisteae, Fabaceae) in the Canary Islands and MadeiraAmer. J. Bot.89854864Google Scholar
  67. Posada, D., Buckley, T. R. 2004Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio testsSyst. Biol.53793808PubMedCrossRefGoogle Scholar
  68. Price, H. J., Dillon, S. L., Hodnett, G., Rooney, W., Ross, L., Johnston, J. S. 2005Genome evolution in the genus Sorghum (Poaceae)Ann. Bot.95219227PubMedCrossRefGoogle Scholar
  69. R Project. (2005) The R Project for Statistical Computing. Scholar
  70. Sang, T., Crawford, D. J., Kim, S. C., Stuessy, T. F. 1994Radiation of the endemic genus Dendroseris (Asteraceae) on the Juan Fernández Islands: evidence from sequences of the ITS regions of nuclear ribosomal DNAAmer. J. Bot.8114941501CrossRefGoogle Scholar
  71. Sang, T., Crawford, D. J., Stuessy, T. F., Silva, M. 1995ITS sequences and phylogeny of the genus Robinsonia (Asteraceae)Syst. Bot.205564CrossRefGoogle Scholar
  72. Silvertown, J. 2004The ghost of competition past in the phylogeny of island endemic plantsJ. Ecol.92168173CrossRefGoogle Scholar
  73. Soltis, D. E., Soltis, P. S., Bennett, D., Leitch, I. J. 2003Evolution of genome size in the AngiospermsAmer. J. Bot.9015961603Google Scholar
  74. Stebbins, G. L. 1971Chromosomal evolution in higher plantsEdward Arnold (Publishers) Ltd.LondonGoogle Scholar
  75. Stübing, G., Peris, J. B., Olivares, A., Martín, J. 1997 Cheirolophus mansanetianus Stübing, Peris, Olivares & Martín, sp. nov. and Ch. grandifolius (Font Quer) comb. & stat. nov. (Asteraceae), two endemics from SpainAnales Jard. Bot. Madrid55170173Google Scholar
  76. Suda, J., Kyncl, T., Freiová, R. 2003Nuclear DNA amounts in Macaronesian AngiospermsAnn. Bot.92153164PubMedCrossRefGoogle Scholar
  77. Suda, J., Kyncl, T., Jarolimová, V. 2005Genome size variation in Macaronesian angiosperms: forty percent of the Canarian endemic flora completedPl. Syst. Evol.252215238CrossRefGoogle Scholar
  78. Susanna, A., Garcia-Jacas, N., Soltis, D. E., Soltis, P. S. 1995Phylogenetic relationships in tribe Cardueae (Asteraceae) based on ITS sequencesAmer. J. Bot.8210561068CrossRefGoogle Scholar
  79. Susanna, A., Garnatje, T., Garcia-Jacas, N. 1999Molecular phylogeny of Cheirolophus (Asteraceae: Cardueae-Centaureinae) based on ITS sequences of nuclear ribosomal DNAPl. Syst. Evol.214147160CrossRefGoogle Scholar
  80. Valdés-Bermejo, E., Agudo, M. P. 1983Estudios cariológicos en especies ibéricas del género Centaurea L. (Compositae)Ann. Jard. Bot. Madrid40119142Google Scholar
  81. Vargas, P., Morton, C. M., Jury, S. L. 1999Biogeographic patterns in Mediterranean and Macaronesian species of Saxifraga (Saxifragaceae) inferred from phylogenetic analyses of ITS sequencesAmer. J. Bot.86724734CrossRefGoogle Scholar
  82. Wagenitz G., Hellwig F. H. (1996) Evolution of characters and phylogeny of the Centaureinae. In: Hind D. J. N., Beentje H. G. (eds.). Compositae: Systematics. Proceedings of the International Compositae Conference, Kew, 1994. Royal Botanic Gardens, Kew, pp. 491–510.Google Scholar
  83. Wendel, J. F., Cronn, R. C., Johnston, J. S., Price, H. J. 2002Feast and famine in plant genomesGenetica1153747PubMedCrossRefGoogle Scholar
  84. Yang Z. (2004) PAML User's Guide. Scholar
  85. Zonneveld, B. J. M., Iren, F. V. 2001Genome size and pollen viability as taxonomic criteria: application to the genus Hosta Pl. Biol.50176185CrossRefGoogle Scholar

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© Springer-Verlag Wien 2006

Authors and Affiliations

  1. 1.Institut Botànic de Barcelona (CSIC-Ajuntament de Barcelona)BarcelonaSpain
  2. 2.Laboratori de Botànica, Facultat de FarmàciaUniversitat de BarcelonaBarcelonaSpain
  3. 3.Departament de Matemàtica Aplicada i Anàlisi, Facultat de MatemàtiquesUniversitat de BarcelonaBarcelonaSpain

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