Plant Systematics and Evolution

, Volume 258, Issue 1–2, pp 85–95 | Cite as

Karyotype relationships among four South American species of Urvillea (Sapindaceae: Paullinieae)

  • J. D. Urdampilleta
  • M. S. Ferrucci
  • J. M. D. Torezan
  • A. L. L. Vanzela


A cytogenetic study was conducted on four species of the genus Urvillea (Sapindaceae, Paullinieae): U. chacoensis Hunz., U. filipes Radlk. and U. ulmacea Kunth of the Urvillea section and U. laevis Radlk. of the Stenelytron section. The chromosome numbers in U. chacoensis (2n = 22) and U. laevis (2n = 24) were confirmed, and new chromosome numbers are reported for U. filipes with 2n = 22 and U. ulmacea with 2n = 88. Additionally, data on interphase nuclear structure, chromosome banding patterns (C-Giemsa and C-CMA3/DAPI) and FISH with rDNA probes are also presented. The distribution of AT- and GC-rich regions and the physical mapping of ribosomal genes (45S and 5S rDNA sites) were established for the first time in these Urvillea species. Sections of Urvillea are cytogenetically differentiated according to basic chromosome number, where x = 11 in the section Urvillea and x = 12 in the section Stenelytron. This first section displayed an important karyotypic feature, the occurrence of large AT- and GC-rich bands at terminal chromosomal regions. The Urvillea section showed polyploidy and its species were differentiated by their banding patterns. Urvillea chacoensis showed several terminal AT-rich bands, while terminal AT- and GC-rich bands were both found in U. ulmacea. However, the section Stenelytron did not exhibit this banding pattern. The 45S rDNA sites appeared always associated with GC-rich regions and they were numerically variable among species, being located or not the same chromosome 5S rDNA sites. Variation in the repetitive DNA distribution and their role in karyotype differentiation among these Urvillea species are discussed.


Banding pattern FISH heterochromatin Paullinieae rDNA Urvillea 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Acevedo, Rodríguez P. 1993Systematics of Serjania (Sapindaceae). Part I: A revision of Serjania Sect. PlatycoccusMem. New York Bot. Gard67193Google Scholar
  2. Barkley, F. A. 1957Sapindaceae of southern South AmericaLilloa28111179Google Scholar
  3. Cuadrado, A., Jouve, N. 1994Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding in 6x-TriticaleChrom. Res2231338Google Scholar
  4. Ferrucci, M. S. 1981Recuentos cromosómicos en SapindáceasBonplandia57381Google Scholar
  5. Ferrucci M. S. (1997a) Urvillea peruviana (Sapindaceae) una nueva especie andina. Bonplandia 9: 237–240.Google Scholar
  6. Ferrucci M. S. (1997b) El número cromosómico de Urvillea laevis (Sapindaceae). Bonplandia 9: 305–306.Google Scholar
  7. Ferrucci M. S. (2000a) Cytotaxonomy of Sapindaceae with special reference to the tribe Paullinieae. Genet. Mol. Biol. 23: 941–946.Google Scholar
  8. Ferrucci M. S. (2000b) Revisión de los géneros de Cardiospermum y Urvillea para el neotrópico (Sapindaceae). Tesis Doctoral. Universidad Nacional de Córdoba, Córdoba, Argentina.Google Scholar
  9. Ferrucci, M. S., Solís, Neffa V. G. 1997Citotaxonomía de Sapindaceae sudamericanasBol. Soc. Argent. Bot337783Google Scholar
  10. Gerlach, W. L., Bedbrook, J. R. 1979Cloning and characterization of ribosomal RNA genes from wheat and barleyNucleic Acids Res718691885PubMedGoogle Scholar
  11. Gerlach, W. L., Dyer, T. A. 1980Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genesNucleic Acids Res848514865PubMedGoogle Scholar
  12. Guerra, M. S. 1983O uso do Giemsa em Citogenética Vegetal: comparação entre a coloração simples e o bandamentoCiência e Cultura35190193Google Scholar
  13. Guerra, M. S. 2000Patterns of heterochromatin distribution in plant chromosomesGenet. Mol. Biol2310291041Google Scholar
  14. Heslop-Harrison, J. S., Schwarzacher, T., Anamthewat-Jonsson, K., Leitch, A. R., Shi, M., Leitch, I. J. 1991In situ hybridization with automated chromosome denaturationTechnique3109116Google Scholar
  15. Hemmer, W., Morawetz, W. 1990Karyological differentiation in Sapindaceae with special reference to Serjania and CardiospermumBot. Acta103372383Google Scholar
  16. Lombello, A. R., Forni-Martins, E. R. 1998Chromosomal studies and evolution in SapindaceaeCaryologia518193Google Scholar
  17. Morawetz, W. 1986Remarks on karyological differentiation patterns in tropical woody plantsPl. Syst. Evol15249100CrossRefGoogle Scholar
  18. Nogueira, Zampieri C., Ruas, P. M., Ruas, C. F., Ferrucci, M. S. 1995Karyotypic study of some species of Serjania and Urvillea (Sapindaceae; Tribe Paullinieae)Amer. J. Bot82646654Google Scholar
  19. Radlkofer, L. 1932SapindaceaeEngler, A. eds. pflanzenr 98 (IV, 165)Wilhelm EngelmannLeipzig352370Google Scholar
  20. Schwarzacher, T. P., Ambros, P., Schweizer, D. 1980Application of Giemsa banding to orchid karyotype analysisPl. Syst. Evol134293297CrossRefGoogle Scholar
  21. Schweizer, D., Loidl, J. 1987A model for heterochromatin dispersion and the evolution of C-band patternsChrom. Today96174Google Scholar
  22. Solís, Neffa V. G., Ferrucci, M. S. 2001Karyotype analyses of some Paullinieae species (Sapindaceae)Caryologia54371376Google Scholar
  23. Vanzela, A. L. L., Ruas, C. F., Oliveira, M. F., Ruas, P. M. 2002Characterization of diploid, tetraploid and hexaploid Helianthus species by chromosome banding and FISH with 45S rDNA probeGenetica114105111CrossRefPubMedGoogle Scholar
  24. Weckerle, C. S., Rutishauser, R. 2005Gynoecium, fruit and seed structure of Paullinieae (Sapindaceae)Bot. J. Linn. Soc147159189CrossRefGoogle Scholar
  25. Wendel, J. F. 2000Genome evolution in polyploidsPlant Molec. Biol42225249Google Scholar

Copyright information

© Springer-Verlag Wien 2006

Authors and Affiliations

  • J. D. Urdampilleta
    • 1
  • M. S. Ferrucci
    • 2
  • J. M. D. Torezan
    • 1
  • A. L. L. Vanzela
    • 1
  1. 1.Laboratório de Biodiversidade e Restauração de Ecossistemas, Departamento de Biologia Geral, CCBUniversidade Estadual de LondrinaParanáBrazil
  2. 2.Instituto de Botánica del Nordeste, Facultad de Ciencias AgrariasUniversidad Nacional del NordesteCorrientesArgentina

Personalised recommendations