Plant Systematics and Evolution

, Volume 257, Issue 3–4, pp 205–222 | Cite as

The rare, endemic zinc violets of Central Europe originate from Viola lutea Huds

  • U. Hildebrandt
  • K. Hoef-Emden
  • S. Backhausen
  • H. Bothe
  • M. Bożek
  • A. Siuta
  • E. Kuta
Article

Abstract

Two endemic zinc violets of the section Melanium Ging. occur on heavy metal soils of Central Europe. The form with yellow flowers is restricted to the area between Aachen, Germany, and Liège, Belgium, whereas the blue zinc violet exclusively thrives on a very small location at Blankenrode, Germany. Both violets are currently treated as separate species. Sequences of altogether 674 bp of the ITS1-5.8S rDNA-ITS2 regions of 61 different specimens of six taxa indicated that both violets are closely related to each other and also to Viola lutea Huds. Therefore these two zinc violets are, at best, subspecies or even only varieties of V. lutea. Thus they are termed V. lutea ssp. westfalica and V. lutea ssp. calaminaria in the present manuscript. Microsporogenesis, pollen morphology and viability of the zinc violets, particularly of the blue violet of Blankenrode, are often defective due to disturbed meiosis. The population of the blue violet might not yet be stabilized genetically but can cope with the adverse effects of the heavy metal elements.

Keywords

Heavy metal plants Zinc violets Phylogeny ITS1-5.8S rDNA-ITS2 regions Microsporogenesis Pollen analysis Viola lutea 

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References

  1. Ballard, H. E., Sytsma, K. J., Kowal, R. R. 1999Shrinking the violets: Phylogenetic relationships of infrageneric groups in Viola (Violaceae) based on internal transcribed spacer DNA sequencesSyst. Bot23439458Google Scholar
  2. Biskup, A., Izmaiłow, R. 2004Endosperm development in seeds of Echium vulgare L. (Boraginaceae) from polluted sitesActa Biol. Cracov. Ser. Bot463944Google Scholar
  3. Bennett, M. D. 2004Perspectives on polyploidy in plants – ancient and neoBiol. J. Linn. Soc82411423CrossRefGoogle Scholar
  4. Braun A. (1854) Über das Vorkommen von Zink im Pflanzenreich. Ber. Bekanntm. Verh. Königl. Preuss. Akad. Wiss. Berlin, Jan. 1854: 12–15.Google Scholar
  5. Clausen, J. 1931Cyto-genetic and taxonomic investigations on Melanium violetsHereditas15219308Google Scholar
  6. Czapik R. (ed). (2002) Embryological and cytological variability of plants in polluted environment. Pol. Bot. Stud. 15, pp. 72.Google Scholar
  7. Dajoz, I. 1999The distribution of pollen heteromorphism in Viola: Ecological and morphological correlatesEvol. Ecol. Res197107Google Scholar
  8. Doyle, J. J., Doyle, J. L. 1990Isolation of plant DNA from fresh tissueFocus121315Google Scholar
  9. Erben, M. 1996The significance of hybridization on the forming of species in the genus ViolaBocconea5113118Google Scholar
  10. Ernst, W. H. O. 1968Das Violetum calaminariae westfalicum, eine Schwermetallpflanzen-Gesellschaft bei Blankenrode in WestfalenMitteilungen der Floristisch-soziologischen Arbeitsgeme- inschaft13263268Google Scholar
  11. Ernst W. H. O. (1999) Evolution of plants on soils anthropogenically contaminated by heavy metals, pp. 13–27. In: Van Raamsdonk L. W. den Nijs J. C. M. (eds.) Plant evolution in man-made habitats. Proceedings VIIth Symposium IOPB, Amsterdam. Hugo de Vries laboratory, Amsterdam.Google Scholar
  12. Fothergill, P. G. 1938Studies in Viola, 1: The cytology of a naturally-occurring population of hybrids between Viola tricolor L. and Viola lutea HudsGenetica20159186CrossRefGoogle Scholar
  13. Gadella, T. W. J. 1963A cytotaxonomic study of Viola in the NetherlandsActa Bot. Neerl121739Google Scholar
  14. Galtier, N., Gouy, M., Gautier, C. 1996SEAVIEW and PHYLO_WIN: Two graphic tools for sequence alignment and molecular phylogenyCABIOS12543548PubMedGoogle Scholar
  15. Grant, V. 1981Plant speciationColumbia University PressNew YorkGoogle Scholar
  16. Griesinger, R. 1937Über hypo- und hyperdiploide Formen von Petunia, Hyoscyamus, Lamium und einige andere ChromosomenzählungenBer. Dt. Bot. Ges55556571Google Scholar
  17. Haeupler H., Schönfelder P. (1988) Atlas der Farnund Blütenpflanzen der Bundesrepublik Deutschland, Ulmer, Stuttgart.Google Scholar
  18. Hasegawa, M., Kishino, H., Yano, T. 1985Dating of the human-ape splitting by a molecular clock of mitochondrial DNAJ. Molec. Evol21160174Google Scholar
  19. Hegi G. (1965) Illustrierte Flora von Mittel-Europa. T.V. Carl Hauser. München.Google Scholar
  20. Heimans, J. 1936De herkomst van de zink-flora aan de GeulNederl. Kruidk. Arch46878893Google Scholar
  21. Hildebrandt, U., Kaldorf, M., Bothe, H. 1999The zinc violet and its colonization by arbuscular mycorrhizal fungiJ. Plant Physiol154709717Google Scholar
  22. Jedrzejczyk M., Rostański A. (2001) Zinc spoil heap – a habitat for introduced taxa Viola guestphalica Nauenb. and Viola calaminaria (Ging.) Lej. Nat. Silesiae Super. Suppl. 53–54.Google Scholar
  23. Kaldorf, M., Kuhn, A. J., Schröder, W. H., Hildebrandt, U., Bothe, H. 1999Selective element deposits in maize colonized by a heavy metal tolerance conferring arbuscular mycorrhizal fungusJ. Plant Physiol154718728Google Scholar
  24. Kakes, P. 1977Genecological investigations on zinc plants II. Introgression in a small population of the zinc violet Viola calaminaria ssp. westfalica (Lej.) ErnstActa Bot. Neerl26385400Google Scholar
  25. Kakes, P. 1979Genecological investigations on zinc plants III. Cytology of hybrids between Viola calaminaria (Lej.) Ernst and Viola arvensis MurrGenetica51135142Google Scholar
  26. Kakes, P., Everards, K. 1976Genecological investigations on zinc plants I. Genetics of flower colour in crosses between Viola calaminaria Lej. and subspecies westfalica (Lej.) ErnstActa Bot. Neerl253140Google Scholar
  27. Krahulcová, A., Krahulec, F., Kirschner, J. 1996Introgressive hybridization between a native and an introduced species: Viola lutea subsp. sudetica versus V. tricolorFolia Geobot. Phytotax31219244Google Scholar
  28. Landwehr, M., Hildebrandt, U., Wilde, P., Nawrath, K., Tóth, T., Biró, B., Bothe, H. 2002The arbuscular mycorrhizal fungus Glomus geosporum in European saline, sodic and gypsum soilsMycorrhiza12199211CrossRefPubMedGoogle Scholar
  29. Lindholm, P., Göransson, U., Johansson, S., Claeson, P., Gullbo, J., Larsson, R., Bohlin, L., Backlund, A. 2002Cyclotides: A novel type of cytotoxic agentsMol. Cancer Therap1365369Google Scholar
  30. Marcussen, T., Borgen, L. 2000Allozymic variation and relationships within Viola subsection Viola (Violaceae)Pl. Syst. Evol2232957CrossRefGoogle Scholar
  31. Nadot, S., Ballard, H. E.,Jr., Crech, J. B., Dajoz, I. 2000The evolution of pollen heteromorphism in Viola: A phylogenetic approachPl. Syst. Evol223155171CrossRefGoogle Scholar
  32. Nauenburg J. D. (1986) Untersuchungen zur Variabilität, Ökologie und Systematik der Violatricolor – Gruppe in Mitteleuropa. PhD dissertation, Göttingen, Germany, 126 p.Google Scholar
  33. Nauenburg J.D. (1987) Viola × preywischiana einzig bei Blankenrode (Westfalen) Floristische Rundbriefe Göttingen 21: 2–3Google Scholar
  34. Posada, D., Crandall, K. A. 1998Modeltest: testing the model of DNA substitutionBioinformatics14817818CrossRefPubMedGoogle Scholar
  35. Rodríguez, F.J., Oliver, J. L., Marín, A., Medina, J. R. 1990The general stochastic model of nucleotide substitutionJ. Theor. Biol142485501PubMedGoogle Scholar
  36. Ronquist, F., Huelsenbeck, J. P. 2003MrBayes 3: Bayesian phylogenetic inference under mixed modelsBioinformatics1915721574CrossRefPubMedGoogle Scholar
  37. Runge F. (1972) Die Flora Westfalens, 2nd ed. Verlag Westfälische Vereinsdruckerei Münster (Westf.).Google Scholar
  38. Schwickerath, M. 1931Das Violetum calaminariae der Zinkböden in der Umgebung AachensBeiträge Naturdenkmalpflege14463503Google Scholar
  39. Singh, R. J. 2003Plant Cytogenetics. 2nd edCRC PressBoca Raton London New York Washington, D.CGoogle Scholar
  40. Siuta, A., Bożek, M., Jedrzejczyk, M., Rostański, A., Kuta, E. 2005Is the blue zinc violet (Viola guestphalica Nauenb.) a taxon of hybrid origin? Evidence from embryologyActa Biol. Cracov. Ser. Bot47237245Google Scholar
  41. Svangard, E., Goransson, U., Hocaoglu, Z., Gullbo, J., Larsson, R., Claeson, P., Bohlin, L. 2004Cytotoxic cyclotides from Viola tricolorJ. Nat. Prod67144147CrossRefPubMedGoogle Scholar
  42. Swofford, D. L. 2002PAUP*: Phylogenetic analyses using parsimony (* and other methods). 4.0Beta for Linux/UnixSinauer AssociatesSunderland, MAGoogle Scholar
  43. Thompson, D. J., Gibson, J. T., Plewniak, F., Jeanmougin, F., Higgins, D. G. 1997The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Res2548764882CrossRefPubMedGoogle Scholar
  44. Tonin, C., Vandenkoornhuse, P., Joner, E. J., Stracek, J., Leyval, C. 2001Assessment of arbuscular mycorrhizal fungi diversity in the rhizosphere of Viola calaminaria and effect of these fungi on heavy metal uptake by cloverMycorrhiza10161168CrossRefGoogle Scholar
  45. Valentine, D. H. 1975

    Viola L

    Stace, C.A. eds. Hybridization and the flora of the British IslesThe Botanical Society of the British Isles & Academic PressLondon New York San Francisco
    Google Scholar
  46. White, T. J., Bruns, T., Lee, S., Taylor, J. 1990

    Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

    Innis, M. A.Gelfand, D. H.Sninsky, J. J.White, T. J. eds. PCR protocols: a guide to methods and applicationsAcademic PressNew York315322
    Google Scholar
  47. Wierzbicka, M., Rostański, A. 2002Microevolutionary changes in ecotypes of calamine heap vegetation near Olkusz, Poland: a reviewActa Biol. Cracov. Ser. Bot44719Google Scholar
  48. Wisskirchen, R., Haeupler, H. 1998Standardliste der Farn- und Blütenpflanzen DeutschlandsUlmerStuttgartGoogle Scholar
  49. Yockteng, R., Ballard, H. E.,Jr., Mansion, G., Dajoz, I., Nadot, S. 2003Relationships among pansies (Viola section Melanium) investigated using ITS an ISSR markersPl. Syst. Evol241153170CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2006

Authors and Affiliations

  • U. Hildebrandt
    • 1
  • K. Hoef-Emden
    • 1
  • S. Backhausen
    • 1
  • H. Bothe
    • 1
  • M. Bożek
    • 2
  • A. Siuta
    • 2
  • E. Kuta
    • 2
  1. 1.Institute of BotanyThe University of CologneCologneGermany
  2. 2.Department of Plant Cytology and EmbryologyJagiellonian UniversityCracowPoland

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