Plant Systematics and Evolution

, Volume 256, Issue 1–4, pp 159–170 | Cite as

Evolution of Dystaenia takesimana (Apiaceae), endemic to Ullung Island, Korea

  • M. PfosserEmail author
  • G. Jakubowsky
  • P. M. Schlüter
  • T. Fer
  • H. Kato
  • T. F. Stuessy
  • B.-Y. Sun


Dystaenia (Apiaceae) consists of two species, one distributed in Japan (D. ibukiensis), and the other endemic to Ullung Island, Korea (D. takesimana). In comparison with representative outgroup taxa in Ligusticum, Seseli, Angelica, and Osmorhiza, Dystaenia is shown to be monophyletic based on sequences from chloroplast trnL-F intron and spacer regions confirming previously published results using ITS sequences. Loss of one large part of trn L-F in D. takesimana strongly suggests that this species evolved from D. ibukiensis rather than the reverse. AFLP analysis within and among twelve populations (six from each species; total 126 individuals) using three primer combinations reveals 130 reliable fragments. Neighbour-joining analysis shows the two species to be distinct populational systems. Levels of overall genetic variation as measured by Shannon Diversity are significantly higher in D. takesimana. Geographic structuring of genetic variation occurs within D. ibukiensis but not within D. takesimana, suggesting that the Ullung species exists as a single population. It is hypothesised that after a founder-effect reduction of genetic variation, anagenetic speciation may have occurred in D. takesimana by gradual morphological divergence accompanied by accumulation of genetic variation through mutation, recombination and drift.


AFLP anagenesis Dystaenia evolution genetic diversity island biology Japan trnL-F Ullung Island 


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  1. Arano, H, Saito, H 1980Cytological studies in the family Umbelliferae II. Karyotypes of some genera of Bupleurum, Spuriopimpinella, and PimpinellaKromosoma2178185Google Scholar
  2. Barber J. C., Francisco-Ortega J., Santos-Guerra A., Marrero A., Jansen R. K. (2000) Evolution of endemic Sideritis (Lamiaceae) in Macaronesia: insights from a chloroplast DNA restriction site analysis. Syst. Bot. 25: 633.647.Google Scholar
  3. Barrett, S. C. H, Kohn, J. R 1991Genetic and evolutionary consequences of small population sizes in plants: implications for conservationFalk, D. AHolsinger, K. E eds. Genetics and conservation of rare plantsOxford University PressNew York330Google Scholar
  4. Carlquist, SBaldwin, B. GCarr, G. D eds. 2003Tarweeds & silverswords: evolution of the Madiinae (Asteraceae)Missouri Bot. Gard. PressSt. LouisGoogle Scholar
  5. Casgrain P., Legendre P. (2001) The R package for multivariate and spatial analyses, version 4.0 d6 – Users Manual. Département de Sciences Biologiques, Université de Montréal. Google Scholar
  6. Choi, H. -K, Kim, Y, Sun, B. -Y, Shin, H 1998Phylogeny of Dystaenia in subfamily Apioideae (Family Apiaceae) based on ITS sequencesKor. J. Plant Tax28139149Google Scholar
  7. Crawford , D. J, Sang, T, Stuessy, T. F, Kim, S. -C, Silva, O. M 1998Dendroseris (Asteraceae: Lactuceae) and Robinsonia (Asteraceae: Senecioneae) on the Juan Fernandez Islands: similarities and differences in biology and phylogenyStuessy, T. FOno, M eds. Evolution and speciation of island plantsCambridge University PressCambridge97120Google Scholar
  8. Cruden, R. W 1977Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plantsEvolution313246Google Scholar
  9. Cruden, R. W 2000Pollen grains: why so manyPl. Syst. Evol222143165CrossRefGoogle Scholar
  10. Doyle, J. J, Doyle, J. L 1987A rapid DNA isolation procedure for small amounts of fresh leaf tissuePhytochem. Bull191115Google Scholar
  11. Emerson, B. C 2002Evolution on oceanic islands: molecular phylogenetic approaches to understanding pattern and processMolec. Ecol11951966Google Scholar
  12. Felsenstein, J 1985Confidence limits on phylogenies: an approach using the bootstrapEvolution39783791Google Scholar
  13. Fitch, W. M 1971Toward defining the course of evolution: minimum change for a specific tree topologySyst. Zool20406416Google Scholar
  14. Genetics Computer Group (1994) Program manual for the Wisconsin package, Version 8. GCG, Madison, Wisconsin, USA. Google Scholar
  15. Grant P. R. (ed.) (1998) Evolution on islands. Oxford University Press, Oxford.Google Scholar
  16. Helfgott, D. M, Francisco-Ortega, J, Jansen, R. K, Simpson, B. B 2000Biogeography and breeding system evolution of the woody Bencomia alliance (Rosaceae) in Macaronesia based on ITS sequence dataSyst. Bot258297Google Scholar
  17. Ishida, T. A, Hattori, K, Sato, H, Kimura, M. T 2003Differentiation and hybridization between Quercus crispula and Q dentata(Fagaceae): insights from morphological traits, amplified fragment length polymorphism markers, and leaf miner compositionAmer. J. Bot90769776Google Scholar
  18. Ito, M, Soejima, A, Ono, M 1998Genetic diversity of the endemic plants of Bonin (Ogasawara) IslandsStuessy, T. FOno, M eds. Evolution and speciation of island plantsCambridge University PressCambridge141154Google Scholar
  19. Jensen, R. J, Schwoyer, M, Crawford, D. J, Stuessy, T. F, Anderson, G. J, Baeza, C. M, Silva, O. M, Ruiz, E 2002Patterns of morphological and genetic variation among populations of Myrceugenia fernandeziana (Myrtaceae) on Masatierra Island: implications for conservationSyst. Bot27534547Google Scholar
  20. Kim, Y. K 1985Petrology of Ulreung volcanic island, Korea 1. Geology. J. Jap. AssocMineral., Petrol. & Econ. Geol80128135Google Scholar
  21. Kim, J. -W 1988The phytosociology of forest vegetation on Ulreung-do, KoreaPhytocoenologia16259281Google Scholar
  22. Kitagawa, M 1937Miscellaneous notes on Apiaceae (Umbelliferae) of Japan and ManchuraiBot. Mag51805812(Tokyo)Google Scholar
  23. Lee W. T., Yang I. S. (1981) The flora of Ulreung and Dogo Island. In: Anon (eds.) A Report on the Scientific Survey of the Ulreung and Dogdo Islands. The Korean Association for Conservation of Nature, Seoul, pp. 61–95.Google Scholar
  24. Legendre, P, Legendre, L 1998Numerical ecologyElsevierAmsterdamGoogle Scholar
  25. Loveless, M. D, Hamrick, J. L 1984Ecological determinants of genetic structure in plant populationsAnnual Rev. Ecol. Syst156595CrossRefGoogle Scholar
  26. Maddison, W. P, Maddison, D. R 1992MacClade: Analysis of phylogeny and character evolution. Version 3.0. Sinauer AssociatesSunderlandMassachusettsGoogle Scholar
  27. Mort, M. E, Soltis, D. E, Soltis, P. S, Francisco-Ortega, J, Santos-Guerra, A 2002Phylogenetics and evolution of the Macaronesian clade of Crassulaceae inferred from nuclear and chloroplast sequence dataSyst. Bot27271288Google Scholar
  28. Nakai T. (1928) The vegetation of Dagelet Island: Its formation and floral relationship with Korea and Japan. Proc. 3rd Pan-Pacific Sci. Congr. 1: 911–914. Tokyo.Google Scholar
  29. Nei, M, Li, W. H 1979Mathematical model for studying genetic variation in terms of restriction endonucleasesProc. Natl. Acad. Sci. USA7652695273PubMedGoogle Scholar
  30. Nielsen, L. R, Cowan, R. S, Siegismund, H. R, Adsersen, H, Philipp, M, Fay, M. F 2003Morphometric, AFLP and plastid microsatellite variation in populations of Scalesia divisa and S. incisa (Asteraceae) from the Galapagos IslandsBot. J. Linn. Soc143243254CrossRefGoogle Scholar
  31. Pfosser, M. F, Guzy-Wróbelska, J, Sun, B. -Y, Stuessy, T. F, Sugawara, T, Fujii, N 2002The origin of species of Acer (Sapindaceae) endemic to Ullung Island, KoreaSyst. Bot27351367Google Scholar
  32. Pfosser M., Sun B. -Y., Stuessy T. F., Jang C. -G., Guo Y. -P., Kim T., Hwan K. C., Kato H., Sugawara T. (2005) Phylogeny of Hepatica (Ranunculaceae) and origin of Hepatica maxima Nakai endemic to Ullung Island, Korea. Biol. J. Linn. Soc. (in press).Google Scholar
  33. Schneider, S, Roessli, D, Excoffier, L 2000Arlequin ver. 2.000: A software for population genetics data analysis. Genetics and Biometry LaboratoryUniversity of GenevaSwitzerlandGoogle Scholar
  34. Schönswetter, P, Tribsch, A, Schneeweiss, G. M, Niklfeld, H 2003Disjunctions in relict alpine plants: phylogeography of Androsace brevis and A wulfeniana (Primulaceae)Bot. J. Linn. Soc141437446Google Scholar
  35. Schönswetter, P, Tribsch, A, Niklfeld, H 2004Glacial history of high alpine Ranunculus glacialis (Ranunculaceae) in the European Alps in a comparative phylogeographical contextBiol. J. Linn. Soc81183195Google Scholar
  36. Stuessy, T. F, Crawford, D. J, Marticorena, C 1990Patterns of phylogeny in the endemic vascular flora of the Juan Fernandez Islands, ChileSyst. Bot15338346Google Scholar
  37. Stuessy, T. F, Crawford, D. J, Marticorena, C, Silva, O. M 1998Isolating mechanisms and modes of speciation in endemic angiosperms of the Juan Fernandez IslandsStuessy, T. FOno, M eds. Evolution and speciation of island plantsCambridge University PressCambridge7998Google Scholar
  38. Stuessy T. F., Ono M. (eds.) (1998) Evolution and speciation of island plants. Cambridge University Press, Cambridge. Google Scholar
  39. Suk, K. -T, Nitta, A, Konoshima, M 1974Studies on origin of Japanese Chuanxiong III. DystaeniaYakugaku Zasshi9412511256PubMedGoogle Scholar
  40. Sun, B. -Y, Park, J. H, Kim, C. H, Kim, K. S 1997Morphological divergence of the genus Dystaenia (Apiaceae)Kor. J. Plant Tax27481490Google Scholar
  41. Sun, B. -Y, Stuessy, T. F 1998Preliminary observations on the evolution of endemic angiosperms of Ullung Island, KoreaStuessy, T. FOno, M eds. Evolution and speciation of island plantsCambridge University PressCambridge181202Google Scholar
  42. Swofford, D. L 2003PAUP*. Phylogenetic analyses using parsimony (* and other methods). Version 4. Sinauer AssociatesSunderlandMassachusettsGoogle Scholar
  43. Tremetsberger, K, Stuessy, T. F, Guo, Y. -P, Baeza, C. M, Weiss, H, Samuel, R. M 2003Amplified Fragment Length Polymorphism (AFLP) variation within and among populations of Hypochaeris acaulis (Asteraceae) of Andean southern South AmericaTaxon52237245Google Scholar
  44. Vos, P, Hogers, R, Bleeker, M, Reijans, M, Lee, T, Hornes, M, Frijtes, A, Pot, J, Peleman, J, Kuiper, M, Zabeau, M 1995AFLP: a new technique for DNA fingerprintingNucl. Acids Res2344074414PubMedGoogle Scholar
  45. Wagner W. L., Funk V. A. (eds.) (1995) Hawaiian biogeography: evolution on a hot spot archipelago. Smithsonian Institution, Washington, D.C. Google Scholar
  46. Weiss, H, Sun, B. -Y, Stuessy, T. F, Kim, C. H, Kato, H, Wakabayashi, M 2002Karyology of plant species endemic to Ullung Island (Korea) and selected relatives in peninsular Korea and JapanBot. J. Linn. Soc13893105CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2005

Authors and Affiliations

  • M. Pfosser
    • 1
    Email author
  • G. Jakubowsky
    • 2
  • P. M. Schlüter
    • 2
  • T. Fer
    • 3
  • H. Kato
    • 4
  • T. F. Stuessy
    • 2
  • B.-Y. Sun
    • 5
  1. 1.Center of BiologyLandesmuseumLinzAustria
  2. 2.Department of Higher Plant Systematics and Evolution, Institute of BotanyUniversity of ViennaViennaAustria
  3. 3.Department of Botany, Faculty of ScienceCharles UniversityPraha (Prague)Czech Republic
  4. 4.Makino HerbariumTokyo Metropolitan UniversityTokyoJapan
  5. 5.Faculty of Biological Sciences, College of Natural SciencesChonbuk National UniversityChonjuSouth Korea

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