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Plant Systematics and Evolution

, Volume 234, Issue 1–4, pp 1–13 | Cite as

Genome size and the nucleolar number as estimators of ploidy level in Dactylis glomeratain the Slovenian Alps

  • B. Vilhar
  • T. Vidic
  • N. Jogan
  • M. Dermastia

Abstract.

 We studied five natural populations of Dactylis glomerata L. (Poaceae) growing at different altitudes in the south-eastern fringe of the Alps in northern Slovenia to determine the subspecies. The stomatal length, the pollen diameter and chromosome counts were consistent with the tetraploid taxon D. glomerata subsp. glomerata (2n=4x =28). Genome size was measured in 55 individuals. The mean 2C value was 8.6 pg DNA. The mean 2C values of populations growing at different altitudes showed only 2.1% variation, and no correlation was observed between altitude and genome size. In D. nk;glomerata subsp. glomerata eight nucleoli were observed in late telophase, indicating that the nucleolus-organising regions inherited from both diploid parent species are functional. We demonstrate that both genome size and the number of nucleoli may be used to determine the ploidy level as an alternative to chromosome counting.

Key words: Dactylis glomerata, morphology, ploidy, genome size, DNA image cytometry, Ag-NOR staining, altitude, nucleolus. 

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Copyright information

© Springer-Verlag Wien 2002

Authors and Affiliations

  • B. Vilhar
    • 1
  • T. Vidic
    • 1
  • N. Jogan
    • 1
  • M. Dermastia
    • 1
  1. 1.University of Ljubljana, Biotechnical Faculty, Department of Biology, Ljubljana, SloveniaSI

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