Advertisement

Microchimica Acta

, Volume 137, Issue 3–4, pp 221–227 | Cite as

Determination of Inorganic Sb(V) and Methylantimony Species by HPLC with Hydride Generation-Atomic Fluorescence Spectrometric Detection

  • Peter J. Craig
  • Tatiania Sergeeva
  • Richard O. Jenkins

Abstract.

 An on-line method for the separation and analysis of Sb(V) and Me3Sb in the presence of Sb(III) in liquid samples is described. Inorganic and organic antimony species were separated using anion-exchange high-performance liquid chromatography (HPLC) coupled with hydride generation-atomic fluorescence detection (HG-AFS). Optimum conditions for the separation of antimony species by HPLC and the hydride generation conditions for the determination by HG-AFS were established. Matrix interference of the chromatographic determination was studied in relation to MgSO4 and NaCl. The method developed was applied to the separation and determination of antimony species in spiked and natural water samples. The suitability of the method for analysis in microbial growth media and physiological studies involving methylantimony species is discussed.

Key words: Antimony; methylantimony; analysis; hydride generation; HPLC; Atomic Fluorescence Spectrometry (AFS). 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Wien 2001

Authors and Affiliations

  • Peter J. Craig
    • 1
  • Tatiania Sergeeva
    • 3
  • Richard O. Jenkins
    • 2
  1. 1.Department of Chemistry, De Montfort University, The Gateway, Leicester LE1 9BH, UKGB
  2. 2.Department of Biological Sciences, De Montfort University, The Gateway, Leicester UK LE1 9BH, UKGB
  3. 3.Now at Department of Chemistry, Moscow State University, 119899 RO, Moscow, RussiaRU

Personalised recommendations