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Iminodiacetic acid (IDA)-generated mesoporous nanopolymer: a template to relate surface area, hydrophilicity, and glycopeptides enrichment

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Abstract

A three-step strategy is introduced to develop inherent iminodiacetic (IDA)-functionalized nanopolymer. SEM micrographs show homogenous spherical beads with a particle size of 500 nm. Further modification to COOH-functionalized 1,2-epoxy-5-hexene/DVB mesoporous nanopolymer enriches glycopeptides via hydrophilic interactions followed by their MS determination. Significantly high BET surface area 433.4336 m2 g−1 contributes to the improved surface hydrophilicity which is also shown by high concentration of ionizable carboxylic acids, 14.59 ± 0.25 mmol g−1. Measured surface area is the highest among DVB-based polymers and in general much higher in comparison to the previously reported BET surface areas of co-polymers, terpolymers, MOFs, and graphene-based composites. Thirty-one, 19, and 16 N-glycopeptides are enriched/identified by nanopolymer beads from tryptic digests of immunoglobulin G, horseradish peroxidase, and chicken avidin, respectively, without additional desalting steps. Material exhibits high selectivity (1:400 IgG:BSA), sensitivity (down to 0.1 fmol), regeneration ability up to three cycles, and batch-to-batch reproducibility (RSD > 1%). Furthermore, from 1 μL of digested human serum, 343 N-glycopeptide characteristics of 134 glycoproteins including 30 FDA-approved serum biomarkers are identified via nano-LC–MS/MS. The developed strategy to self-generate IDA on polymeric surface with improved surface area, porosity, and ordered morphology is insignia of its potential as chromatographic tool contributing to future developments in large-scale biomedical glycoproteomics studies.

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Data is available via the ProteomeXchange with identifier PXD027783.

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Funding

This work is supported by the Higher Education Commission (HEC) of Pakistan.

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Authors and Affiliations

  1. Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Muhammad Salman Sajid, Fahmida Jabeen, Muhammad Naeem Ashiq & Muhammad Najam-ul-Haq

  2. Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide, South Australia, 5005, Australia

    Muhammad Salman Sajid & Tara Louise Pukala

  3. Department of Chemistry, The Women University, Kutchery Campus, L.M.Q. Road, Multan, 66000, Pakistan

    Shafaq Saleem & Fahmida Jabeen

  4. Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Batool Fatima

  5. Department of Chemical Engineering, COMSATS University Islamabad, Lahore Campus, Lahore, 54000, Pakistan

    M. Zulfikar

  6. Department of Oncology, Genomics and Epigenomics Shared Resource, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, 20057, USA

    Habtom W. Ressom

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  1. Muhammad Salman Sajid
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  2. Shafaq Saleem
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  9. Muhammad Najam-ul-Haq
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Correspondence to Muhammad Najam-ul-Haq.

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Sajid, M.S., Saleem, S., Jabeen, F. et al. Iminodiacetic acid (IDA)-generated mesoporous nanopolymer: a template to relate surface area, hydrophilicity, and glycopeptides enrichment. Microchim Acta 188, 417 (2021). https://doi.org/10.1007/s00604-021-05074-8

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  • DOI: https://doi.org/10.1007/s00604-021-05074-8

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